Click here to switch to the map view.

The map label for this gene is mdtO [H]

Identifier: 218551507

GI number: 218551507

Start: 4393591

End: 4395597

Strand: Direct

Name: mdtO [H]

Synonym: EFER_4290

Alternate gene names: 218551507

Gene position: 4393591-4395597 (Clockwise)

Preceding gene: 218551506

Following gene: 218551508

Centisome position: 95.75

GC content: 51.77

Gene sequence:

>2007_bases
ATGAGCGCGCTCAACTACCTTCCGCTACCAATGGTTCGGCTGTTGGCTTTTCTTCATGAGGAGTTAAACGAACAGCGACC
GGGGCGTATGGCACAAACCTTCCAGCTATGGGCTGGCTGTCTGCTGGTTGTGCTGATCTCCATGACGTTTGAGATCCCGT
TTATGGCGATCTCACTGGCTGTTCTGTTTTATGGTGTGCAGACAAATGCCTTCTATACCAAATTTGTGGCGATCCTGTTT
GTGGTTGCTACGGTGCTGGAAATGGGTAGCCTGTTTTTGATCTATAAATGGTCTTACGGCTACCCGCTGATGCGTCTGGT
GATTGCCGGGCCGATATTGATGGGCTGCATGTTTATGATGCGTACCCATCGGCTGGGGTTGGTCTTTTTTGCTGTGGCAA
TTGTCGCTATTTACGGGCAAACCTTCCCGGCGATGATCGATGTGCCGGAAATTGTTGTACGTCTGACGCTGTGGTGCATC
GTGGTTGGGCTTTACCCGACCTTGCTGATGACGTTAATCGGTGTGTTGTGGTTTCCCAGCCGGGCAGTAAATCAGATGCA
TAAAGCGCTAAGCGAGCGTCTGGATGATGCGTTACGTCATCTCACCAACGAGGTTTCACCGTTAGCAGAAAAATATATTG
AACGGGAAGCGCTGGCGCTGCAAAAGCTGAACGTCTTCTGTCTAGCCGATGACGCGGAGTGGCGTAAGAAAAGCGCCTGG
TGGCAGAACTGTGTTGCTACGGTCACTTATCTCTATACCAGCCTGAATCGTTACGATGTCCGTGCCTTCCCGGAATCCCC
GGAAATGACTGTTTTACGTCAGAAGCTGATCAGTGAAGTGCAGTCGCTGCAACAAGCCATTTGCGCAGGTGAAACCTGGC
AAAGCAACTGGCAGCTTCGGGAAGATGAACTTACGCTTGCTCATCAATGTGGTTTGTTCAGCCATTGTCAGATGTTGCTG
CAACTGGGGCATATGGACCCAGCGACACCCCCCACACCAGCAGCAAAACCGCCATCAATGGTCGCGGATGCATTTACCAA
TCCTGATTATTTGCGTTATGCCCTGAAAACATTGCTGGCATGTTTGATTTGCTACACCTTTTATTCCGGTGTCGATTGGG
AAGGTATTCACACCTGTATGCTGACCTGCGTGATTGTCGCTAACCCCAATATTGGCTCGTCATATCAGAAAATGGCATTA
CGTTTTGGTGGCGCATTCTGTGGGGCGATTCTGGCGCTGGTGGTCACTATTTTTGTGATGCCGTGGCTGGACAATATTGT
CGAGTTACTGTGCGTGCTGGCCCCCATTTTCTGGATTGGCGCAAGGCTGGCAACCAGTTCAGAGCGTTCGTCTTATATCG
GCACCCAGATGGTCGTCACTTTTGCCCTCGCCACGCTGGAAAACGTCTTTGGCCCGGCGTATGACCTCGTGGAGATCCGC
GATCGTGCTATCGGTATTTTGATCGGAACGGCAGTTTCAGCAGTCATTTACACTTTTGTCTGGCCGGAAAGTGAAGCACG
CGCTTTACCACAAAAACTGGCCGGAGCAATGGCGATGCTCAGCAAACTGCTGCGTATTCCACGCCAGCCGGAAGCCGCCA
TGGAACGAACTTATCTGCAATTGCGGATTGGCTTACATGCAGCGTTCAACGCCAGTGAAGAGATGTATGAACGCGTCGCG
CTTGAGCGTCAGCTTGCAGGAGCAGAACGGGAAGCTCTCCTGCAGCGCGCGAAGAGTGTCATTACCCTTGGTCGGGAAAT
TATTCACACCTGGGATAATACCGGGATTAACTCGCTTACCCCTGAGCGCGTGAATCAACTTGCCGGAGCGCTGGAATTTT
ACGCCGCAGGATTGCCACAACATACCAGCCTTTCCCCCACGCTTGACATGAGTGAATTAAATCAACAGGAGCAGCGCGTC
ATGCAGTTAATCGCCAGTCTACCTGACTGGACTTCCCCAACGCTTACGCCTGCCACGCAACACGTGCAAGGAGCTACACC
ATCATGA

Upstream 100 bases:

>100_bases
GTGTTGCCCAGCGTTTCCCGGTAAAAATCATGGTAGATAACCCTGACCCTGAGATGTTCCGTATAGGTGCTTCGGCGGTT
GCTAACCTTGAGCCACAATA

Downstream 100 bases:

>100_bases
GCCGTCATCTTTCCCGCTTAATTCTCTGTGGTCTGTTAGGCGGCGCGACGCTGCTTTCTGGCTGTGCCCTGGTACGCAAA
GATTCTGCGCCGCATCAGCA

Product: multidrug efflux system protein MdtO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MSALNYLPLPMVRLLAFLHEELNEQRPGRMAQTFQLWAGCLLVVLISMTFEIPFMAISLAVLFYGVQTNAFYTKFVAILF
VVATVLEMGSLFLIYKWSYGYPLMRLVIAGPILMGCMFMMRTHRLGLVFFAVAIVAIYGQTFPAMIDVPEIVVRLTLWCI
VVGLYPTLLMTLIGVLWFPSRAVNQMHKALSERLDDALRHLTNEVSPLAEKYIEREALALQKLNVFCLADDAEWRKKSAW
WQNCVATVTYLYTSLNRYDVRAFPESPEMTVLRQKLISEVQSLQQAICAGETWQSNWQLREDELTLAHQCGLFSHCQMLL
QLGHMDPATPPTPAAKPPSMVADAFTNPDYLRYALKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNIGSSYQKMAL
RFGGAFCGAILALVVTIFVMPWLDNIVELLCVLAPIFWIGARLATSSERSSYIGTQMVVTFALATLENVFGPAYDLVEIR
DRAIGILIGTAVSAVIYTFVWPESEARALPQKLAGAMAMLSKLLRIPRQPEAAMERTYLQLRIGLHAAFNASEEMYERVA
LERQLAGAEREALLQRAKSVITLGREIIHTWDNTGINSLTPERVNQLAGALEFYAAGLPQHTSLSPTLDMSELNQQEQRV
MQLIASLPDWTSPTLTPATQHVQGATPS

Sequences:

>Translated_668_residues
MSALNYLPLPMVRLLAFLHEELNEQRPGRMAQTFQLWAGCLLVVLISMTFEIPFMAISLAVLFYGVQTNAFYTKFVAILF
VVATVLEMGSLFLIYKWSYGYPLMRLVIAGPILMGCMFMMRTHRLGLVFFAVAIVAIYGQTFPAMIDVPEIVVRLTLWCI
VVGLYPTLLMTLIGVLWFPSRAVNQMHKALSERLDDALRHLTNEVSPLAEKYIEREALALQKLNVFCLADDAEWRKKSAW
WQNCVATVTYLYTSLNRYDVRAFPESPEMTVLRQKLISEVQSLQQAICAGETWQSNWQLREDELTLAHQCGLFSHCQMLL
QLGHMDPATPPTPAAKPPSMVADAFTNPDYLRYALKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNIGSSYQKMAL
RFGGAFCGAILALVVTIFVMPWLDNIVELLCVLAPIFWIGARLATSSERSSYIGTQMVVTFALATLENVFGPAYDLVEIR
DRAIGILIGTAVSAVIYTFVWPESEARALPQKLAGAMAMLSKLLRIPRQPEAAMERTYLQLRIGLHAAFNASEEMYERVA
LERQLAGAEREALLQRAKSVITLGREIIHTWDNTGINSLTPERVNQLAGALEFYAAGLPQHTSLSPTLDMSELNQQEQRV
MQLIASLPDWTSPTLTPATQHVQGATPS
>Mature_667_residues
SALNYLPLPMVRLLAFLHEELNEQRPGRMAQTFQLWAGCLLVVLISMTFEIPFMAISLAVLFYGVQTNAFYTKFVAILFV
VATVLEMGSLFLIYKWSYGYPLMRLVIAGPILMGCMFMMRTHRLGLVFFAVAIVAIYGQTFPAMIDVPEIVVRLTLWCIV
VGLYPTLLMTLIGVLWFPSRAVNQMHKALSERLDDALRHLTNEVSPLAEKYIEREALALQKLNVFCLADDAEWRKKSAWW
QNCVATVTYLYTSLNRYDVRAFPESPEMTVLRQKLISEVQSLQQAICAGETWQSNWQLREDELTLAHQCGLFSHCQMLLQ
LGHMDPATPPTPAAKPPSMVADAFTNPDYLRYALKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNIGSSYQKMALR
FGGAFCGAILALVVTIFVMPWLDNIVELLCVLAPIFWIGARLATSSERSSYIGTQMVVTFALATLENVFGPAYDLVEIRD
RAIGILIGTAVSAVIYTFVWPESEARALPQKLAGAMAMLSKLLRIPRQPEAAMERTYLQLRIGLHAAFNASEEMYERVAL
ERQLAGAEREALLQRAKSVITLGREIIHTWDNTGINSLTPERVNQLAGALEFYAAGLPQHTSLSPTLDMSELNQQEQRVM
QLIASLPDWTSPTLTPATQHVQGATPS

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1289

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MdtO family [H]

Homologues:

Organism=Escherichia coli, GI87082367, Length=682, Percent_Identity=73.7536656891496, Blast_Score=1015, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006726 [H]

Pfam domain/function: PF04632 FUSC [H]

EC number: NA

Molecular weight: Translated: 74765; Mature: 74633

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALNYLPLPMVRLLAFLHEELNEQRPGRMAQTFQLWAGCLLVVLISMTFEIPFMAISLA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFYGVQTNAFYTKFVAILFVVATVLEMGSLFLIYKWSYGYPLMRLVIAGPILMGCMFMM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RTHRLGLVFFAVAIVAIYGQTFPAMIDVPEIVVRLTLWCIVVGLYPTLLMTLIGVLWFPS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RAVNQMHKALSERLDDALRHLTNEVSPLAEKYIEREALALQKLNVFCLADDAEWRKKSAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
WQNCVATVTYLYTSLNRYDVRAFPESPEMTVLRQKLISEVQSLQQAICAGETWQSNWQLR
HHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EDELTLAHQCGLFSHCQMLLQLGHMDPATPPTPAAKPPSMVADAFTNPDYLRYALKTLLA
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
CLICYTFYSGVDWEGIHTCMLTCVIVANPNIGSSYQKMALRFGGAFCGAILALVVTIFVM
HHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWLDNIVELLCVLAPIFWIGARLATSSERSSYIGTQMVVTFALATLENVFGPAYDLVEIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DRAIGILIGTAVSAVIYTFVWPESEARALPQKLAGAMAMLSKLLRIPRQPEAAMERTYLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LRIGLHAAFNASEEMYERVALERQLAGAEREALLQRAKSVITLGREIIHTWDNTGINSLT
HHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PERVNQLAGALEFYAAGLPQHTSLSPTLDMSELNQQEQRVMQLIASLPDWTSPTLTPATQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
HVQGATPS
HCCCCCCC
>Mature Secondary Structure 
SALNYLPLPMVRLLAFLHEELNEQRPGRMAQTFQLWAGCLLVVLISMTFEIPFMAISLA
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFYGVQTNAFYTKFVAILFVVATVLEMGSLFLIYKWSYGYPLMRLVIAGPILMGCMFMM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RTHRLGLVFFAVAIVAIYGQTFPAMIDVPEIVVRLTLWCIVVGLYPTLLMTLIGVLWFPS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RAVNQMHKALSERLDDALRHLTNEVSPLAEKYIEREALALQKLNVFCLADDAEWRKKSAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
WQNCVATVTYLYTSLNRYDVRAFPESPEMTVLRQKLISEVQSLQQAICAGETWQSNWQLR
HHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EDELTLAHQCGLFSHCQMLLQLGHMDPATPPTPAAKPPSMVADAFTNPDYLRYALKTLLA
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
CLICYTFYSGVDWEGIHTCMLTCVIVANPNIGSSYQKMALRFGGAFCGAILALVVTIFVM
HHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWLDNIVELLCVLAPIFWIGARLATSSERSSYIGTQMVVTFALATLENVFGPAYDLVEIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DRAIGILIGTAVSAVIYTFVWPESEARALPQKLAGAMAMLSKLLRIPRQPEAAMERTYLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LRIGLHAAFNASEEMYERVALERQLAGAEREALLQRAKSVITLGREIIHTWDNTGINSLT
HHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PERVNQLAGALEFYAAGLPQHTSLSPTLDMSELNQQEQRVMQLIASLPDWTSPTLTPATQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
HVQGATPS
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]