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The map label for this gene is yjfC [H]

Identifier: 218551456

GI number: 218551456

Start: 4346389

End: 4347552

Strand: Direct

Name: yjfC [H]

Synonym: EFER_4239

Alternate gene names: 218551456

Gene position: 4346389-4347552 (Clockwise)

Preceding gene: 218551455

Following gene: 218551457

Centisome position: 94.72

GC content: 46.74

Gene sequence:

>1164_bases
ATGTTAAGACATGATATTTCTGTACGTCGCGATCTGGATCGAATTGCCGCCGATCATGGCTTCGACTTTCATATTATCGA
CGATGAAATCTATTGGGATGAGAGCAGAGCATACCGTTTCACCCTCAGGCAAATTGAAGAACAGATTGAGAAACCGACCG
CAGAGCTGCATCAGATGTGTCTTGACGTTGTGGATCGTGCTGTTAAAGATGAGCAGATTATGCAGCAGCTGGCGATCCCA
CCACTATATTGGGATGTGATCGCTGAAAGTTGGCGTGCCAGAGATCCTTCGCTGTATGGTCGGATGGATTTTGTCTGGTG
CGGTAATGCGCCTGTAAAATTGCTCGAATACAACGCTGACACACCCACCTCATTGTATGAAGCATCTTACTTTCAGTGGT
TGTGGCTGGAAGATGCTCGACGTAGTGGTGTAATTCCCCGTGATGCCGATCAGTACAACGCAATTCAGGAGCGTCTGATC
TCACGCTTTGCTGAGCTTTATAGTCGCGAGCCTTTTTATTTCTGCTGTTGCCAGGATACCGACGAAGACCGTACCACTGT
TCTGTACTTGCAGGATTGCGCACAACAGGCAGGCCAGGAATCCCGGTTTATTTATATCGAAGATCTTGGACTTGGTGTTG
GTGGGGTACTGACAGATCTGGATGATAATGTGATTAAACGTGCATTCAAGCTTTATCCGCTGGAGTGGATGATGCGTGAT
GATAATGGCCCACTATTGCGTTCTCGTCGTGAACAGTGGGTGGAACCGCTATGGAAAAGTATCCTCAGTAACAAAGGGCT
GCTACCGCTGCTATGGCGCTTTTTCCCCCATCATCCCAACTTGTTACCTGCCTGGTTTGAAGGTGAAAAATCACAAGTCA
ATGCAGGTGAAAACTATGTGCGAAAACCTATATTTTCTCGTGAGGGTGGTAATGTCACTATCTTTGACGGTAATAATGCC
GTTATAGATCATGCTGAGGGTGATTACGCCGATGAGCCAATGATTTATCAGGCATTTCAGCCTCTGCCGCGTTTTGGTGA
TAGCTACACACTGATCGGCAGCTGGATTGTTGATGATGAAGCATGTGGCATGGGTATCCGCGAAGATAACACCTTGATCA
CAAAAGATACTTCACGTTTTGTTCCTCATTACATTTCTGGCTAG

Upstream 100 bases:

>100_bases
CAGCCAGCACCAGTAGTTTTTCAACGAAAAAGGCGTCGACTGTCTCCCGTGGCGGTTATGGGCGTTCGTCCAGTGCGCGT
GGGCATTGGGGAGGCTGATC

Downstream 100 bases:

>100_bases
GAATGTGTTAGCGATTCCTCTCTGTCATGAATGAATGGCAGTGACCATACTTAAGGTTACTGCCATTGATGGAGGGAGAC
ATAGTGCACTGGCAAACTCA

Product: synthetase/amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLRHDISVRRDLDRIAADHGFDFHIIDDEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLDVVDRAVKDEQIMQQLAIP
PLYWDVIAESWRARDPSLYGRMDFVWCGNAPVKLLEYNADTPTSLYEASYFQWLWLEDARRSGVIPRDADQYNAIQERLI
SRFAELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIKRAFKLYPLEWMMRD
DNGPLLRSRREQWVEPLWKSILSNKGLLPLLWRFFPHHPNLLPAWFEGEKSQVNAGENYVRKPIFSREGGNVTIFDGNNA
VIDHAEGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYISG

Sequences:

>Translated_387_residues
MLRHDISVRRDLDRIAADHGFDFHIIDDEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLDVVDRAVKDEQIMQQLAIP
PLYWDVIAESWRARDPSLYGRMDFVWCGNAPVKLLEYNADTPTSLYEASYFQWLWLEDARRSGVIPRDADQYNAIQERLI
SRFAELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIKRAFKLYPLEWMMRD
DNGPLLRSRREQWVEPLWKSILSNKGLLPLLWRFFPHHPNLLPAWFEGEKSQVNAGENYVRKPIFSREGGNVTIFDGNNA
VIDHAEGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYISG
>Mature_387_residues
MLRHDISVRRDLDRIAADHGFDFHIIDDEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLDVVDRAVKDEQIMQQLAIP
PLYWDVIAESWRARDPSLYGRMDFVWCGNAPVKLLEYNADTPTSLYEASYFQWLWLEDARRSGVIPRDADQYNAIQERLI
SRFAELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIKRAFKLYPLEWMMRD
DNGPLLRSRREQWVEPLWKSILSNKGLLPLLWRFFPHHPNLLPAWFEGEKSQVNAGENYVRKPIFSREGGNVTIFDGNNA
VIDHAEGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYISG

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli ygiC [H]

Homologues:

Organism=Escherichia coli, GI1790629, Length=387, Percent_Identity=91.4728682170543, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1789416, Length=387, Percent_Identity=52.1963824289406, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1789361, Length=374, Percent_Identity=28.3422459893048, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: NA

Molecular weight: Translated: 45181; Mature: 45181

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRHDISVRRDLDRIAADHGFDFHIIDDEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC
CCCCCHHHHHHHHHHHHCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHH
LDVVDRAVKDEQIMQQLAIPPLYWDVIAESWRARDPSLYGRMDFVWCGNAPVKLLEYNAD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCEEEEEECCCCEEEEEECCC
TPTSLYEASYFQWLWLEDARRSGVIPRDADQYNAIQERLISRFAELYSREPFYFCCCQDT
CCHHHHHCCCEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIKRAFKLYPLEWMMRD
CCCCEEEEEHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHHCCEEEEEEC
DNGPLLRSRREQWVEPLWKSILSNKGLLPLLWRFFPHHPNLLPAWFEGEKSQVNAGENYV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHH
RKPIFSREGGNVTIFDGNNAVIDHAEGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE
HCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCH
ACGMGIREDNTLITKDTSRFVPHYISG
HHCCCCCCCCEEEECCCHHHCCHHCCC
>Mature Secondary Structure
MLRHDISVRRDLDRIAADHGFDFHIIDDEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC
CCCCCHHHHHHHHHHHHCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHH
LDVVDRAVKDEQIMQQLAIPPLYWDVIAESWRARDPSLYGRMDFVWCGNAPVKLLEYNAD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCEEEEEECCCCEEEEEECCC
TPTSLYEASYFQWLWLEDARRSGVIPRDADQYNAIQERLISRFAELYSREPFYFCCCQDT
CCHHHHHCCCEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIKRAFKLYPLEWMMRD
CCCCEEEEEHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHHCCEEEEEEC
DNGPLLRSRREQWVEPLWKSILSNKGLLPLLWRFFPHHPNLLPAWFEGEKSQVNAGENYV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHH
RKPIFSREGGNVTIFDGNNAVIDHAEGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE
HCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCH
ACGMGIREDNTLITKDTSRFVPHYISG
HHCCCCCCCCEEEECCCHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7961409 [H]