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The map label for this gene is aceK [H]

Identifier: 218551293

GI number: 218551293

Start: 4171259

End: 4172992

Strand: Direct

Name: aceK [H]

Synonym: EFER_4069

Alternate gene names: 218551293

Gene position: 4171259-4172992 (Clockwise)

Preceding gene: 218551292

Following gene: 218551294

Centisome position: 90.9

GC content: 52.54

Gene sequence:

>1734_bases
ATGCCGCGTGGCCTGGAATTACTGATTGCACAAACTATTTTGCAAGGTTTCGACGCTCAGTATGGTCGATTTCTTGAAGT
GACTTCCGGGGCGCAGCAGCGTTTCGAACAAGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAGCCGTATCCATCTTT
ACGATCATCACGTCGGTCTGGTAGTGGAGCAACTGCGCTGCATTACCAACGGCCAAAGCACGGACGCGGCATTTTTACTG
CGCGTCAAAGAGCATTACACCCGGCTGTTGCCGGACTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTA
CTGTCGGTTATTTGATCACCGTTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGTTCCCAGCCAGAGCGCCGCTTTCGTA
CTATTCCGCGCCCGCTGGCAAAAGACTTTTATCCCGATCACGGCTGGGAATCTCTGCTAATGCGCGTTATCAGCGATCTG
CCGCTGCGTCTGCGCTGGCAAAATAAAAGCCGTGACATTCATTACATCATTCGCCATCTGACAGAAATGTTGGGGACAGA
CAACCTCGCGGAAAGCCATTTACAGGTGGCGAACGAACTGTTTTATCGCAATAAAGCCGCCTGGCTGGTGGGCAAACTGA
ACACGCCTTTCGGCACATTGCCATTTTTGCTGCCGATCCATCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTG
ACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCT
GGTCGAGTGGCTACGGGAAATTCTGCCGGGCAAAACGACCGCAGAATTGTATATGGCTATCGGTTGCCAGAAACACGCCA
AAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGC
ATGGTGATGTTGGTGTTTACGCTGCCGGGTTTTGATCGGGTATTCAAAGTCATCAAAGACAAGTTCGCGCCGCAGAAAGA
GATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTTAAAGAGCACGATCGCGTGGGTCGAATGGCGGACACCCAGG
AGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGACATTAATGGAATTACTGCTTCAGGAAGCAGCAGAAAAA
ATCACCGATCTCGGCGAACAGATAGTGATTCGTCATCTTTATATTGAGCGACGGATGGTGCCGCTCAATATCTGGCTGGA
ACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTT
TCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTGTTTTATGATTACGATGAAATTTGC
TACATGACGGAAGTGAACTTCCGCGACATCCCGCCGTCGCGCTACCCGGAGGACGAACTTGCCAGCGAACCGTGGTACAG
CGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGTGCCGACCCGCGTATTGGTCCGCTGTTTGAAG
AGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTT
TATGCGTATCGGCGCAGGCAGCGATTTAGCGTGCGTTACCGGCCACAGGTGTAG

Upstream 100 bases:

>100_bases
CACGTCGTCGGTTACCGCGCTGACCGGCTCCACCGAAGAATCGCAGTTCTAATAACCAACGCCCCTTTCCGTAAGGCGAG
GGGCGAATTAAGGAGAGGCG

Downstream 100 bases:

>100_bases
TCACATTTTATCTGGGTCAAATGAGTGATATATTTTCACCCACTAATTCCGGGAGAAAATATGACTGAATTCGATCTGTT
AGCTCAGGAGCTGCTGGAGA

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGLVVEQLRCITNGQSTDAAFLL
RVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPDHGWESLLMRVISDL
PLRLRWQNKSRDIHYIIRHLTEMLGTDNLAESHLQVANELFYRNKAAWLVGKLNTPFGTLPFLLPIHQTDDGELFIDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRG
MVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPTLMELLLQEAAEK
ITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPSRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDV
YAYRRRQRFSVRYRPQV

Sequences:

>Translated_577_residues
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGLVVEQLRCITNGQSTDAAFLL
RVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPDHGWESLLMRVISDL
PLRLRWQNKSRDIHYIIRHLTEMLGTDNLAESHLQVANELFYRNKAAWLVGKLNTPFGTLPFLLPIHQTDDGELFIDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRG
MVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPTLMELLLQEAAEK
ITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPSRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDV
YAYRRRQRFSVRYRPQV
>Mature_576_residues
PRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLR
VKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPDHGWESLLMRVISDLP
LRLRWQNKSRDIHYIIRHLTEMLGTDNLAESHLQVANELFYRNKAAWLVGKLNTPFGTLPFLLPIHQTDDGELFIDTCLT
TTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGM
VMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPTLMELLLQEAAEKI
TDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEICY
MTEVNFRDIPPSRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVY
AYRRRQRFSVRYRPQV

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family [H]

Homologues:

Organism=Escherichia coli, GI1790446, Length=573, Percent_Identity=98.6038394415358, Blast_Score=1172, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010452
- ProDom:   PD043552 [H]

Pfam domain/function: PF06315 AceK [H]

EC number: =2.7.11.5 [H]

Molecular weight: Translated: 67684; Mature: 67553

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGL
CCCCHHHHHHHHHHHHCCHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LFIFSSQPERRFRTIPRPLAKDFYPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHL
EEEECCCCHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
TEMLGTDNLAESHLQVANELFYRNKAAWLVGKLNTPFGTLPFLLPIHQTDDGELFIDTCL
HHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEHHHH
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHH
YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLV
HHHHHHCCCHHHHCCCCCCCEEEHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEFENFVLEKRHISPTLMELLLQEAAEKITDLGEQIVIRHLYIERRMV
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPSRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
RADYWRALQNRIREGHVEDVYAYRRRQRFSVRYRPQV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGL
CCCHHHHHHHHHHHHCCHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LFIFSSQPERRFRTIPRPLAKDFYPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHL
EEEECCCCHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
TEMLGTDNLAESHLQVANELFYRNKAAWLVGKLNTPFGTLPFLLPIHQTDDGELFIDTCL
HHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEHHHH
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHH
YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLV
HHHHHHCCCHHHHCCCCCCCEEEHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEFENFVLEKRHISPTLMELLLQEAAEKITDLGEQIVIRHLYIERRMV
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPSRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
RADYWRALQNRIREGHVEDVYAYRRRQRFSVRYRPQV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]