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The map label for this gene is ecoRIIM [H]

Identifier: 218551294

GI number: 218551294

Start: 4173017

End: 4174474

Strand: Direct

Name: ecoRIIM [H]

Synonym: EFER_4070

Alternate gene names: 218551294

Gene position: 4173017-4174474 (Clockwise)

Preceding gene: 218551293

Following gene: 218551296

Centisome position: 90.94

GC content: 44.1

Gene sequence:

>1458_bases
GTGATATATTTTCACCCACTAATTCCGGGAGAAAATATGACTGAATTCGATCTGTTAGCTCAGGAGCTGCTGGAGAAAGC
AGCCAATGATGAAAAAAACCAACAGGAGCGCGATAAACAACTTATCGATCGGGTTCTGGAAATCTACGATCAGAAATACG
TTGCTGAATTACTTAGAAAAATTGGTAAAAATGAGTGGAGTCGGGAAACACTTAACCGCTGGGTAAATGGCAAAAGTAAA
CCAAAAACACTCACTCAGGCGGAAGTTGAGCTATTAAGAAAAATGCTTCCAGAGCCACCAGCCCACCATCCAAATTATGC
GTTTCGGTTTATTGATCTATTTGCCGGAATTGGTGGTATCCGTAAGGGGTTTGAGGCAATTGGAGGTCAATGTGTATTTA
CCAGTGAGTGGAATAAGGAGGCTGTTCGCACTTATAAGGCAAACTGGTATAACGACGAAAGTAGCCATACATTTAATCAT
GATATACGCGAGGTCACGTTAAGCGCCGATGATACGGTAAGTGAAAAACAAGCGTATGCACATATTCAGCGTCATATTCC
TGATCACGACGTATTACTGGCGGGATTTCCTTGCCAGCCATTCAGTCTGGCAGGGGTCAGTAAGAAAAATTCTCTCGGGC
GTGCGCACGGTTTTGAATGCGAAACACAAGGCACTCTATTTTTTGATGTTGCACGGATTATTAAAGCAAAGCGCCCGGCG
ATATTTGTACTGGAAAATGTCAAAAACTTAAAAAGCCATGATAAAGGTAAGACCTTTAAGGTCATTATGAATACCCTTGA
TGAGCTGGGCTATGAAGTGGCCAACGCTCATGAAACCGGGAAGGATGATCCTAAAATCATTGATGGCAAACATTTCCTCC
CGCAGCATCGTGAGCGGATTGTGCTGGTGGGTTTCCGTCGTGATCTCAATATTCATCAAGGTTTTACTTTACGGGATATT
GATCGTTTCTATCCGCAGCAAAGACCGCTCTTTGGTCAACTACTGGAATCCTCTGTTGATAGCAAATACATCCTCAGCCC
AAAGCTCTGGCAATATCTGTATAACTATGCCAAAAAACATGCGGCGAAAGGCAATGGTTTTGGATTTGGGTTGGTTGATC
CAAATAATGCAAATAGCGTCGCCAGGACATTATCTGCCCGTTACCATAAAGATGGCTCAGAAATATTGATCGACCGGGGC
TGGGACAAGGCGCTTGGGGAGATAGATTTTAACAATGCTGAAAATCAGGCGAACAGACCACGCAGATTAACGCCTCGCGA
GTGTGCTCGTCTGATGGGATTTGAATCCCCTGGCGGGAAACCGTTCCGTATACCGGTTTCAGATACACAGGCTTACCGTC
AATTTGGTAACTCAGTCGTCGTACCGGTATTTGCAGCAGTTGCGAAACTGCTTAAGCCCTATATTTTGCAGGCCACCAGT
AAATCGATAAAAAAATAA

Upstream 100 bases:

>100_bases
TGAAGGGCATGTGGAAGATGTTTATGCGTATCGGCGCAGGCAGCGATTTAGCGTGCGTTACCGGCCACAGGTGTAGTCAC
ATTTTATCTGGGTCAAATGA

Downstream 100 bases:

>100_bases
ATTGATCGTTATACCCGGCACGCTCCGGGTATAACGCATCACTTTTCTTACCGCATTCCACCGTATGCCAGCGTCACTTC
CTTCGCGGCTTTAATGACCA

Product: DNA cytosine methylase

Products: NA

Alternate protein names: M.EcoRII; Cytosine-specific methyltransferase EcoRII [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MIYFHPLIPGENMTEFDLLAQELLEKAANDEKNQQERDKQLIDRVLEIYDQKYVAELLRKIGKNEWSRETLNRWVNGKSK
PKTLTQAEVELLRKMLPEPPAHHPNYAFRFIDLFAGIGGIRKGFEAIGGQCVFTSEWNKEAVRTYKANWYNDESSHTFNH
DIREVTLSADDTVSEKQAYAHIQRHIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIKAKRPA
IFVLENVKNLKSHDKGKTFKVIMNTLDELGYEVANAHETGKDDPKIIDGKHFLPQHRERIVLVGFRRDLNIHQGFTLRDI
DRFYPQQRPLFGQLLESSVDSKYILSPKLWQYLYNYAKKHAAKGNGFGFGLVDPNNANSVARTLSARYHKDGSEILIDRG
WDKALGEIDFNNAENQANRPRRLTPRECARLMGFESPGGKPFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLKPYILQATS
KSIKK

Sequences:

>Translated_485_residues
MIYFHPLIPGENMTEFDLLAQELLEKAANDEKNQQERDKQLIDRVLEIYDQKYVAELLRKIGKNEWSRETLNRWVNGKSK
PKTLTQAEVELLRKMLPEPPAHHPNYAFRFIDLFAGIGGIRKGFEAIGGQCVFTSEWNKEAVRTYKANWYNDESSHTFNH
DIREVTLSADDTVSEKQAYAHIQRHIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIKAKRPA
IFVLENVKNLKSHDKGKTFKVIMNTLDELGYEVANAHETGKDDPKIIDGKHFLPQHRERIVLVGFRRDLNIHQGFTLRDI
DRFYPQQRPLFGQLLESSVDSKYILSPKLWQYLYNYAKKHAAKGNGFGFGLVDPNNANSVARTLSARYHKDGSEILIDRG
WDKALGEIDFNNAENQANRPRRLTPRECARLMGFESPGGKPFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLKPYILQATS
KSIKK
>Mature_485_residues
MIYFHPLIPGENMTEFDLLAQELLEKAANDEKNQQERDKQLIDRVLEIYDQKYVAELLRKIGKNEWSRETLNRWVNGKSK
PKTLTQAEVELLRKMLPEPPAHHPNYAFRFIDLFAGIGGIRKGFEAIGGQCVFTSEWNKEAVRTYKANWYNDESSHTFNH
DIREVTLSADDTVSEKQAYAHIQRHIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIKAKRPA
IFVLENVKNLKSHDKGKTFKVIMNTLDELGYEVANAHETGKDDPKIIDGKHFLPQHRERIVLVGFRRDLNIHQGFTLRDI
DRFYPQQRPLFGQLLESSVDSKYILSPKLWQYLYNYAKKHAAKGNGFGFGLVDPNNANSVARTLSARYHKDGSEILIDRG
WDKALGEIDFNNAENQANRPRRLTPRECARLMGFESPGGKPFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLKPYILQATS
KSIKK

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=442, Percent_Identity=69.9095022624434, Blast_Score=635, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 55385; Mature: 55385

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFHPLIPGENMTEFDLLAQELLEKAANDEKNQQERDKQLIDRVLEIYDQKYVAELLRK
CEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGKNEWSRETLNRWVNGKSKPKTLTQAEVELLRKMLPEPPAHHPNYAFRFIDLFAGIGGI
HCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RKGFEAIGGQCVFTSEWNKEAVRTYKANWYNDESSHTFNHDIREVTLSADDTVSEKQAYA
HHHHHHHCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCHHEEEECCCCCHHHHHHHH
HIQRHIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIKAKRPA
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
IFVLENVKNLKSHDKGKTFKVIMNTLDELGYEVANAHETGKDDPKIIDGKHFLPQHRERI
EEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCEE
VLVGFRRDLNIHQGFTLRDIDRFYPQQRPLFGQLLESSVDSKYILSPKLWQYLYNYAKKH
EEEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCEECCHHHHHHHHHHHHHH
AAKGNGFGFGLVDPNNANSVARTLSARYHKDGSEILIDRGWDKALGEIDFNNAENQANRP
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCC
RRLTPRECARLMGFESPGGKPFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLKPYILQATS
CCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KSIKK
HHHCC
>Mature Secondary Structure
MIYFHPLIPGENMTEFDLLAQELLEKAANDEKNQQERDKQLIDRVLEIYDQKYVAELLRK
CEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGKNEWSRETLNRWVNGKSKPKTLTQAEVELLRKMLPEPPAHHPNYAFRFIDLFAGIGGI
HCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RKGFEAIGGQCVFTSEWNKEAVRTYKANWYNDESSHTFNHDIREVTLSADDTVSEKQAYA
HHHHHHHCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCHHEEEECCCCCHHHHHHHH
HIQRHIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECETQGTLFFDVARIIKAKRPA
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
IFVLENVKNLKSHDKGKTFKVIMNTLDELGYEVANAHETGKDDPKIIDGKHFLPQHRERI
EEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCEE
VLVGFRRDLNIHQGFTLRDIDRFYPQQRPLFGQLLESSVDSKYILSPKLWQYLYNYAKKH
EEEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCEECCHHHHHHHHHHHHHH
AAKGNGFGFGLVDPNNANSVARTLSARYHKDGSEILIDRGWDKALGEIDFNNAENQANRP
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCC
RRLTPRECARLMGFESPGGKPFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLKPYILQATS
CCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KSIKK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029675 [H]