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The map label for this gene is gldA [H]

Identifier: 218551063

GI number: 218551063

Start: 3911399

End: 3912502

Strand: Direct

Name: gldA [H]

Synonym: EFER_3817

Alternate gene names: 218551063

Gene position: 3911399-3912502 (Clockwise)

Preceding gene: 218551062

Following gene: 218551064

Centisome position: 85.24

GC content: 53.44

Gene sequence:

>1104_bases
ATGGACCGTATTATTCAATCTCCAGGTAAATACATCCAGGGTGCTGATGTCATTAATCGTCTGGGCGAATACCTGAAACC
ACTGGCAGAACGCTGGTTAGTTGTTGGTGATAAATTTGTATTAGGATTTGCTCAGGCTACCGTAGAGCAAAGCTTTAAAG
ATGCCGGGCTGGTTGTTGAAATCGCACCGTTTGGCGGCGAATGTTCACAAAATGAGATCGACCGTCTGCGCGGCATCGCG
GAGACTGCCCAGTGTGGCGCAATTCTCGGTATCGGTGGTGGTAAAACCCTCGATACCGCTAAAGCACTGGCTCACTACAT
GAGTGTACCGGTTGCAATCGCACCGACCATCGCCTCTACCGACGCACCGTGCAGTGCGTTGTCTGTTATCTACACCGACG
ACGGCGAGTTTGACCGCTACCTGTTGCTGCCAAACAACCCGGAACGCGTTATTGTTGATACCAAAATTGTTGCTGGCGCG
CCTGCACGTCTGTTAGCAGCGGGTATCGGTGATGCGCTGGCAACCTGGTTTGAAGCACGCGCCTGTTCCCGTAGCGGCGC
TACCACTATGGCGGGCGGTAAGTGTACCCAGGCAGCTCTTGCGCTGGCTGAACTGTGCTACAACACTCTGCTGGAAGAAG
GCGAAAAAGCGATGCTTGCCGCCGAACAGCATGTAGTAACCCCTGCGCTGGAACGCGTGATTGAAGCGAACACCTATTTA
AGCGGTGTAGGCTTTGAAAGTGGAGGTCTGGCTGCGGCGCACGCAGTACATAACGGCCTGACCGCTATTCCAGATGCGCA
TCACTATTATCACGGTGAAAAAGTGGCATTTGGTACACTGACGCAGCTGGTACTGGAAAATGCTCCGCTGGAAGAGATCG
AAACTGTTGCCGCCCTGTGCCATTCCGTTGGCCTGCCGATCACGCTGGCGCAACTGGATATTAAAGAAGATATCCCGGCG
AAAATGCGCATTGTTGCGGAAGCATCTTGTGCAGAAGGTGAAACCATCCACAACATGCCTGGCGGCGCAACACCAGATGA
AGTATATGCCGCGCTGCTGGTAGCCGACCAGTACGGTCAGCGTTTCCTGCAAGAGTGGGAATAA

Upstream 100 bases:

>100_bases
CAACAAATGCTCAATACGCCTGCGGTAGAGTCAGCTATAGAGAAGTTTGAACACGACTGGAAAGCTACATTTGGCAATAT
CAATCTCTAAGGGAACTATT

Downstream 100 bases:

>100_bases
TCCACACCAAACTCCCGCGTCAGCGGGAGTTTTTTATCCGCTTCCGGACATTTCCCTCACAGAACATTCAAACGCTCTGA
AGGTCGAATCTGTGAATATT

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQATVEQSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHYMSVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLLLPNNPERVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPLEEIETVAALCHSVGLPITLAQLDIKEDIPA
KMRIVAEASCAEGETIHNMPGGATPDEVYAALLVADQYGQRFLQEWE

Sequences:

>Translated_367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQATVEQSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHYMSVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLLLPNNPERVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPLEEIETVAALCHSVGLPITLAQLDIKEDIPA
KMRIVAEASCAEGETIHNMPGGATPDEVYAALLVADQYGQRFLQEWE
>Mature_367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQATVEQSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHYMSVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLLLPNNPERVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPLEEIETVAALCHSVGLPITLAQLDIKEDIPA
KMRIVAEASCAEGETIHNMPGGATPDEVYAALLVADQYGQRFLQEWE

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=367, Percent_Identity=96.4577656675749, Blast_Score=725, Evalue=0.0,
Organism=Escherichia coli, GI1786815, Length=359, Percent_Identity=31.1977715877437, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 38846; Mature: 38846

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQATVEQSFKDAGLVVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHYMSVPVAIAPTIAST
EECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCC
DAPCSALSVIYTDDGEFDRYLLLPNNPERVIVDTKIVAGAPARLLAAGIGDALATWFEAR
CCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCE
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPLEEIETVAALC
ECCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHH
HSVGLPITLAQLDIKEDIPAKMRIVAEASCAEGETIHNMPGGATPDEVYAALLVADQYGQ
HHCCCCEEEEEECCCCCCCHHEEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
RFLQEWE
HHHHHCC
>Mature Secondary Structure
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQATVEQSFKDAGLVVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHYMSVPVAIAPTIAST
EECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCC
DAPCSALSVIYTDDGEFDRYLLLPNNPERVIVDTKIVAGAPARLLAAGIGDALATWFEAR
CCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCE
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPLEEIETVAALC
ECCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHH
HSVGLPITLAQLDIKEDIPAKMRIVAEASCAEGETIHNMPGGATPDEVYAALLVADQYGQ
HHCCCCEEEEEECCCCCCCHHEEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
RFLQEWE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]