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The map label for this gene is selB [H]

Identifier: 218550868

GI number: 218550868

Start: 3669210

End: 3671057

Strand: Reverse

Name: selB [H]

Synonym: EFER_3584

Alternate gene names: 218550868

Gene position: 3671057-3669210 (Counterclockwise)

Preceding gene: 218550869

Following gene: 218550867

Centisome position: 80.0

GC content: 53.35

Gene sequence:

>1848_bases
ATGATTATTGCTACCGCCGGACACGTAGACCACGGTAAAACCACGCTATTACAGGCAATTACGGGAGTTAACGCCGACCG
CCTGCCAGAAGAAAAGAAGCGTGGCATGACCATCGATCTCGGTTATGCCTACTGGCCGCAGCCAGATGGTCGCGTGTTAG
GTTTTATTGATGTTCCTGGTCACGAAAAATTTCTCTCCAACATGCTGGCTGGTGTGGGCGGTATTGATCATGCGTTGCTG
GTGGTGGCGTGTGATGATGGTGTAATGGCACAAACCCGTGAGCACCTGGCTATTTTGCAGTTAACAGGAAATCCGACACT
GACGGTGGCACTCACTAAAGCGGATCGCGTAGATGAAGCGCGTGTTGGTGAGGTTGAACGCCAGGTAAAGGAGGTGCTGC
GGGAATATGGTTTTGCTGATGCAAAACTGTTTGTCACCGCAGCAACCGAAGGGCGGGGAATTGATGCCCTGCGTGAACAT
TTGCTCCAGTTGCCGGAACGCGAACATGCCAGTCAGCAAAGTTTCCGTCTGGCCATCGACCGTGCTTTTACGGTTAAAGG
GGCAGGACTGGTGGTGACGGGCACGGCACTGAGCGGTGAAGTGAAAGTCGGCGACACGTTATGGCTGACTGGAGTGAATA
AACCGATGCGTGTGCGTGCGCTTCATGCACAAAATCAACCTACTGAATTCGCTCATGCCGGGCAGCGTATTGCCCTGAAT
ATTGCTGGTGATGCAGAAAAAGATCAGATTAGCCGTGGTGACTGGTTACTTACCGATGCACCGCCAGAACCATTCACCCG
GATTATTGTTGAGCTACGCACTCACGAGCCGCTGACCCAGTGGCAGCCACTGCATATTCATCATGCTGCCAGCCACGTTA
CCGGGCGCGTGTCACTGCTGGAAGATAACCTTGCAGAACTGGTCTTCGACACCCCGTTATGGCTGGCGGATAACGACCGC
CTGGTATTGCGCGATATCTCTGCCCGCAACACGCTGGCCGGAGCGCGCGTCGTGATGCTTAACCCGCCGCGTCGTGGGAA
GCGTAAACCAGAGTATCTGCAATGGCTGGCTTCCCTCGCCGGGGCACAGAATGATAGCGAAGCGTTAAGCGTTCACCTGC
AACGCGGCGCGGTTAATCTTCCTGATTTTACCTGGGCGCGGCAGCTGAGTGGTGAAGGATTGCGTCACCTGATTCAGCAG
CCGGGCTTTATTCAGGCCGGTTATAGCTTGTTAAACGCTCCTGTTGCTGCACGCTGGCAACGGAAAATTCTCGATACGCT
GGCAACGTACCATGAACAACATCGTGATGAGCCAGGCCCTGGACGCGAGCGTCTGCGTCGCATGGCACTGCCGATGGAAG
ATGAAGCTCTGGTGCTGTTGCTGATTGAAAAGATGCGCGAAAGTGGGGATATCCATAGCCACCACGGTTGGCTGCATCTG
CCCAGTCACAAAGCCGGGTTTAGCGATGAACAGCAGGTGATCTGGCAAAAAGCCGCACCGTTGTTCGCCGATGAGCCATG
GTGGGTACGCGATCTGGCAAAAGAAACCGGAACAGAAGAGCAGGCAATGCGTCTGACGCTGCGTCAGGCCGCGCAGCAAG
GGATGATTACTGCGATCGTTAAAGATCGTTACTACCGCAATGATCGGATTGTTGAATTCGCCAATATGATCCGCGATCTC
GATCAGCAAACCGGATCAACCTGTGCGGCAGATTTCCGTGATCGCTTAGGTGTTGGACGAAAACTGGCGATTCAGATTCT
GGAATATTTTGACCGTATTGGATTTACCCGTCGTCGAGGAAATGATCATTTATTACGCGATGCGTTATTATTTCCTGAAA
AAGAATGA

Upstream 100 bases:

>100_bases
AATTGCCCGTGCCGGTTATCGGACGGATTTACGATGGCCGCCTGTGGTTGGATTTGCGCTGCCTCGAAGATGAGACCCGG
TTTTTGGAGATGTTGCTGAA

Downstream 100 bases:

>100_bases
AACAATTAATTCAAAATTAAAATGAATTAATACAAATATCTTATTGATATGAATAAAGCACAATAGATTTTTTAATCTAA
TGTGCTTTTTTATTCTTCTC

Product: selenocysteinyl-tRNA-specific translation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPTLTVALTKADRVDEARVGEVERQVKEVLREYGFADAKLFVTAATEGRGIDALREH
LLQLPEREHASQQSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRALHAQNQPTEFAHAGQRIALN
IAGDAEKDQISRGDWLLTDAPPEPFTRIIVELRTHEPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLAGAQNDSEALSVHLQRGAVNLPDFTWARQLSGEGLRHLIQQ
PGFIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDIHSHHGWLHL
PSHKAGFSDEQQVIWQKAAPLFADEPWWVRDLAKETGTEEQAMRLTLRQAAQQGMITAIVKDRYYRNDRIVEFANMIRDL
DQQTGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEKE

Sequences:

>Translated_615_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPTLTVALTKADRVDEARVGEVERQVKEVLREYGFADAKLFVTAATEGRGIDALREH
LLQLPEREHASQQSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRALHAQNQPTEFAHAGQRIALN
IAGDAEKDQISRGDWLLTDAPPEPFTRIIVELRTHEPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLAGAQNDSEALSVHLQRGAVNLPDFTWARQLSGEGLRHLIQQ
PGFIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDIHSHHGWLHL
PSHKAGFSDEQQVIWQKAAPLFADEPWWVRDLAKETGTEEQAMRLTLRQAAQQGMITAIVKDRYYRNDRIVEFANMIRDL
DQQTGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEKE
>Mature_615_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPTLTVALTKADRVDEARVGEVERQVKEVLREYGFADAKLFVTAATEGRGIDALREH
LLQLPEREHASQQSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRALHAQNQPTEFAHAGQRIALN
IAGDAEKDQISRGDWLLTDAPPEPFTRIIVELRTHEPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLAGAQNDSEALSVHLQRGAVNLPDFTWARQLSGEGLRHLIQQ
PGFIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDIHSHHGWLHL
PSHKAGFSDEQQVIWQKAAPLFADEPWWVRDLAKETGTEEQAMRLTLRQAAQQGMITAIVKDRYYRNDRIVEFANMIRDL
DQQTGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEKE

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=371, Percent_Identity=28.8409703504043, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI54607086, Length=284, Percent_Identity=25.3521126760563, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI223555963, Length=378, Percent_Identity=24.6031746031746, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI5729864, Length=378, Percent_Identity=24.6031746031746, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI53729339, Length=286, Percent_Identity=29.7202797202797, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI53729337, Length=286, Percent_Identity=29.7202797202797, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI82546879, Length=298, Percent_Identity=25.8389261744966, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI45593140, Length=323, Percent_Identity=24.7678018575851, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI46094014, Length=383, Percent_Identity=22.7154046997389, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI2367247, Length=614, Percent_Identity=93.8110749185668, Blast_Score=1182, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=386, Percent_Identity=30.0518134715026, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1789737, Length=377, Percent_Identity=30.7692307692308, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1789108, Length=292, Percent_Identity=27.0547945205479, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789559, Length=251, Percent_Identity=29.0836653386454, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=293, Percent_Identity=32.4232081911263, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556456, Length=291, Percent_Identity=32.3024054982818, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=263, Percent_Identity=26.9961977186312, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI115532067, Length=304, Percent_Identity=27.6315789473684, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115532065, Length=304, Percent_Identity=27.6315789473684, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552884, Length=406, Percent_Identity=24.1379310344828, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17569207, Length=406, Percent_Identity=24.1379310344828, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17565438, Length=343, Percent_Identity=23.9067055393586, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=234, Percent_Identity=29.9145299145299, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566629, Length=299, Percent_Identity=25.4180602006689, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=303, Percent_Identity=32.6732673267327, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6325337, Length=402, Percent_Identity=22.8855721393035, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319594, Length=402, Percent_Identity=22.8855721393035, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324550, Length=175, Percent_Identity=28, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363316, Length=281, Percent_Identity=34.8754448398576, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17864358, Length=281, Percent_Identity=34.8754448398576, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19921738, Length=246, Percent_Identity=35.3658536585366, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922690, Length=329, Percent_Identity=27.9635258358663, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45550900, Length=375, Percent_Identity=23.7333333333333, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17137380, Length=350, Percent_Identity=25.1428571428571, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28572034, Length=161, Percent_Identity=33.5403726708075, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45553807, Length=379, Percent_Identity=24.2744063324538, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45553816, Length=379, Percent_Identity=24.2744063324538, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24651721, Length=379, Percent_Identity=24.2744063324538, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17864154, Length=379, Percent_Identity=24.2744063324538, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24652838, Length=405, Percent_Identity=21.4814814814815, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17137572, Length=405, Percent_Identity=21.4814814814815, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 68916; Mature: 68916

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEECEEECCCCCCCEEEEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLTGNPTLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCEEEEEEECCCCCCHH
RVGEVERQVKEVLREYGFADAKLFVTAATEGRGIDALREHLLQLPEREHASQQSFRLAID
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHCCHHHHEEEEE
RAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRALHAQNQPTEFAHAGQRIALN
EEEEEECCCEEEEECEECCCEEECCEEEEECCCCCEEEEEEECCCCCHHHHHCCCEEEEE
IAGDAEKDQISRGDWLLTDAPPEPFTRIIVELRTHEPLTQWQPLHIHHAASHVTGRVSLL
ECCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHCCHHHH
EDNLAELVFDTPLWLADNDRLVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLA
HHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
GAQNDSEALSVHLQRGAVNLPDFTWARQLSGEGLRHLIQQPGFIQAGYSLLNAPVAARWQ
CCCCCCHHEEEEEECCCCCCCCHHHHHHHCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHH
RKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDIHSHHGWLHL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
PSHKAGFSDEQQVIWQKAAPLFADEPWWVRDLAKETGTEEQAMRLTLRQAAQQGMITAIV
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEH
KDRYYRNDRIVEFANMIRDLDQQTGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCC
NDHLLRDALLFPEKE
CCHHHHHHHCCCCCC
>Mature Secondary Structure
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEECEEECCCCCCCEEEEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLTGNPTLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCEEEEEEECCCCCCHH
RVGEVERQVKEVLREYGFADAKLFVTAATEGRGIDALREHLLQLPEREHASQQSFRLAID
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHCCHHHHEEEEE
RAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRALHAQNQPTEFAHAGQRIALN
EEEEEECCCEEEEECEECCCEEECCEEEEECCCCCEEEEEEECCCCCHHHHHCCCEEEEE
IAGDAEKDQISRGDWLLTDAPPEPFTRIIVELRTHEPLTQWQPLHIHHAASHVTGRVSLL
ECCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHCCHHHH
EDNLAELVFDTPLWLADNDRLVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLA
HHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
GAQNDSEALSVHLQRGAVNLPDFTWARQLSGEGLRHLIQQPGFIQAGYSLLNAPVAARWQ
CCCCCCHHEEEEEECCCCCCCCHHHHHHHCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHH
RKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDIHSHHGWLHL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
PSHKAGFSDEQQVIWQKAAPLFADEPWWVRDLAKETGTEEQAMRLTLRQAAQQGMITAIV
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEH
KDRYYRNDRIVEFANMIRDLDQQTGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCC
NDHLLRDALLFPEKE
CCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]