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The map label for this gene is yiaJ [H]

Identifier: 218550856

GI number: 218550856

Start: 3657003

End: 3657875

Strand: Reverse

Name: yiaJ [H]

Synonym: EFER_3572

Alternate gene names: 218550856

Gene position: 3657875-3657003 (Counterclockwise)

Preceding gene: 218550866

Following gene: 218550855

Centisome position: 79.71

GC content: 49.37

Gene sequence:

>873_bases
GTGAACTGCGACACACTTTGCGCTACCATCAGGGCGCGACAAAATGGGAAGCCGAATATGGGGAAAAAAGAGGGCGATAT
GACGCAAGAAAAAGAAAGGCCAGCCGGAAGTCAGAGCCTGTTTCGCGGTTTGATGTTGATTGAGATCCTCAGTAACTATC
CCAACGGATGTCCACTGGCGCTTCTCTCTGAACTGGCAGGGCTGAACAAAAGTACCGTCCACCGTTTATTGCAGGGGCTA
CAGTCCTGCGGTTATGTGACAACAGCTCCGGCAGCGGGTAGCTACCGCCTGACCACCAAATTTATCGCCGTTGGGCAAAA
AGCATTGTCATCGCTGAATATCATTCATATTGCCGCTCCGCATCTTGAGGCGTTGAATATTGCTACCGGTAAAACGATTA
ATTTCTCCAGCCGCGAAGATGATCACGCGATTTTGATTTATAAACTGGAACCCACCACCGGGATGCTGCGAACCCGCGCT
TATATTGGTCAGCATATGCCGCTCTACTGTTCGGCGATGGGCAAAATCTACATGGCGTTTGGTCAGCCGGAATATGTGAA
ATCTTACTGGGAAAGCCATCAGGAGGAGATCCAGCCATTGACGCGCAATACCATCACTGAATTACCTGAGATGTATAAAG
AGTTAGCGCAAATTCGTGAAAGCAGCATGGCGATGGACCGGGAAGAGAATGAAATAGGTGTTTCCTGTATTGCTGTACCT
GTATTTGATATTCACGGGCGGGTGCCGTACGCAGTTTCTATCTCTCTTTCGACGTCACGTCTTAAACAAATCGGCGAAAG
CAATCTGCTTAAGCCGCTACAGGAAACCGCTCAGGCAATTTCCCGCGAGCTTGGCTTTACGGTACGTGATTGA

Upstream 100 bases:

>100_bases
TTTGATTTCAAATATATCGATCGCTATTTAACAAAGCAATCTGATTCATGATTTTAAAAAATAAACAAAATCAATATGTT
GTAAATTATCTGTCGAGATC

Downstream 100 bases:

>100_bases
GCGAACGATCAAAACTTGATGAAATTCTGACAAATTTTTCTCGTACCCCTTTTTTGATCAAGGCGAGAATGATATTTCGT
CTGGCACTCTTAGCGTTTTA

Product: DNA-binding transcriptional repressor

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNCDTLCATIRARQNGKPNMGKKEGDMTQEKERPAGSQSLFRGLMLIEILSNYPNGCPLALLSELAGLNKSTVHRLLQGL
QSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGKTINFSSREDDHAILIYKLEPTTGMLRTRA
YIGQHMPLYCSAMGKIYMAFGQPEYVKSYWESHQEEIQPLTRNTITELPEMYKELAQIRESSMAMDREENEIGVSCIAVP
VFDIHGRVPYAVSISLSTSRLKQIGESNLLKPLQETAQAISRELGFTVRD

Sequences:

>Translated_290_residues
MNCDTLCATIRARQNGKPNMGKKEGDMTQEKERPAGSQSLFRGLMLIEILSNYPNGCPLALLSELAGLNKSTVHRLLQGL
QSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGKTINFSSREDDHAILIYKLEPTTGMLRTRA
YIGQHMPLYCSAMGKIYMAFGQPEYVKSYWESHQEEIQPLTRNTITELPEMYKELAQIRESSMAMDREENEIGVSCIAVP
VFDIHGRVPYAVSISLSTSRLKQIGESNLLKPLQETAQAISRELGFTVRD
>Mature_290_residues
MNCDTLCATIRARQNGKPNMGKKEGDMTQEKERPAGSQSLFRGLMLIEILSNYPNGCPLALLSELAGLNKSTVHRLLQGL
QSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGKTINFSSREDDHAILIYKLEPTTGMLRTRA
YIGQHMPLYCSAMGKIYMAFGQPEYVKSYWESHQEEIQPLTRNTITELPEMYKELAQIRESSMAMDREENEIGVSCIAVP
VFDIHGRVPYAVSISLSTSRLKQIGESNLLKPLQETAQAISRELGFTVRD

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=275, Percent_Identity=92.3636363636364, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=33.8645418326693, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788131, Length=225, Percent_Identity=33.7777777777778, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786715, Length=254, Percent_Identity=29.9212598425197, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI87082359, Length=227, Percent_Identity=33.0396475770925, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790752, Length=217, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788581, Length=236, Percent_Identity=26.6949152542373, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693096, Length=143, Percent_Identity=32.8671328671329, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32126; Mature: 32126

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCDTLCATIRARQNGKPNMGKKEGDMTQEKERPAGSQSLFRGLMLIEILSNYPNGCPLA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHH
LLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAP
HHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECC
HLEALNIATGKTINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAF
CHHHEECCCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHEEEEC
GQPEYVKSYWESHQEEIQPLTRNTITELPEMYKELAQIRESSMAMDREENEIGVSCIAVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
VFDIHGRVPYAVSISLSTSRLKQIGESNLLKPLQETAQAISRELGFTVRD
EEECCCCCCEEEEEECCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure
MNCDTLCATIRARQNGKPNMGKKEGDMTQEKERPAGSQSLFRGLMLIEILSNYPNGCPLA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHH
LLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAP
HHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECC
HLEALNIATGKTINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAF
CHHHEECCCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHEEEEC
GQPEYVKSYWESHQEEIQPLTRNTITELPEMYKELAQIRESSMAMDREENEIGVSCIAVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
VFDIHGRVPYAVSISLSTSRLKQIGESNLLKPLQETAQAISRELGFTVRD
EEECCCCCCEEEEEECCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]