The gene/protein map for NC_011740 is currently unavailable.
Definition Escherichia fergusonii ATCC 35469 chromosome, complete genome.
Accession NC_011740
Length 4,588,711

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The map label for this gene is tiaE

Identifier: 218550838

GI number: 218550838

Start: 3636574

End: 3637548

Strand: Direct

Name: tiaE

Synonym: EFER_3554

Alternate gene names: 218550838

Gene position: 3636574-3637548 (Clockwise)

Preceding gene: 218550837

Following gene: 218550840

Centisome position: 79.25

GC content: 49.64

Gene sequence:

>975_bases
ATGAAGCCGTCCATTATTCTTTATAAAAAGTTACCTGACGTTTTACTGCAACGCCTTGAGGAACACTTTACTGTAACGCA
GGTAAGCAACCTGAGCCCACAAACGGTAGAGCAACACGCAGAAGCGTTTGCGAATGCGGAAGGTCTGTTGGGTTCAAGCG
AAAAAGTGGATGATGCATTACTGGAAAAAATGCCAAAGCTGCGGGCAACGTCAACGATCTCTGTAGGTTATGACAATTTT
GATGTTGATGCCCTTAACGCCCGTAAAGTATTGCTGATGCATACTCCTACAGTGCTGACTGAAACTGTCGCGGATACGTT
GATGGCGCTTATTCTCGCCACTTCTCGTCGGGTGGTGGAAGTCGCAGAACGCGTAAAAGCCGGTGAATGGACTGCCAGCA
TTGGGCCTGACTGGTTTGGTAGTGATGTACATCACAAGACCTTAGGGATTGTTGGCATGGGGCGCATTGGTATGGCACTG
GCCCAGCGTGCACACTTTGGTTTTAACATGCCGATTCTCTATAACGCTCGTCGCCAACACCCGCAGGCAGAAGAGCGCTT
TAACGCCCGCTATTGCGATCTCGATACGTTGTTGCAGGAAGCTGATTTCGTTTGTCTGATTCTGCCGTTAACCGAAGAAA
CGCATCATCTCTTCGGTGCTGAACAGTTTGCCAAAATGAAATCCTCCGCCATTTTCATTAACGCTGGTCGTGGCCCAGTG
GTTGATGAAAAAGCCCTGATAAGCGCATTACAGAAGGGAGAAATTCACGCTGCCGGACTGGATGTGTTTGAGCGGGAACC
ACTGCCAGTAGATTCCCCTTTGCTTGCTATGCCAAACGTGGTGGCACTGCCACATATCGGTTCTGCCACTCATGAAACAC
GTTATAACATGGCGGCTTGTGCGGTGGACAACCTGATCGCAGCGCTACAGGGCAATGTCGATAAGAACTGCGTGAATCCG
CAAGTCGCGAAGTAA

Upstream 100 bases:

>100_bases
TTTCGTTTCTGTATGGCTAACATTGATTGTTAAACCAGCAGCCAATCAACCGCTACCCGTTGGCGCGCGCCACGCTTAAA
TATTAATCACGGAGATTCGC

Downstream 100 bases:

>100_bases
AAAAATACCGGAGGGGTATATCCACTCCGGTATCAGATTGTTGAATAAATAGACTTAATTAATGCCCGAAGCATTATCGG
GCATTAAAATTATTATTCAG

Product: 2-oxo-carboxylic acid reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKPSIILYKKLPDVLLQRLEEHFTVTQVSNLSPQTVEQHAEAFANAEGLLGSSEKVDDALLEKMPKLRATSTISVGYDNF
DVDALNARKVLLMHTPTVLTETVADTLMALILATSRRVVEVAERVKAGEWTASIGPDWFGSDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRQHPQAEERFNARYCDLDTLLQEADFVCLILPLTEETHHLFGAEQFAKMKSSAIFINAGRGPV
VDEKALISALQKGEIHAAGLDVFEREPLPVDSPLLAMPNVVALPHIGSATHETRYNMAACAVDNLIAALQGNVDKNCVNP
QVAK

Sequences:

>Translated_324_residues
MKPSIILYKKLPDVLLQRLEEHFTVTQVSNLSPQTVEQHAEAFANAEGLLGSSEKVDDALLEKMPKLRATSTISVGYDNF
DVDALNARKVLLMHTPTVLTETVADTLMALILATSRRVVEVAERVKAGEWTASIGPDWFGSDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRQHPQAEERFNARYCDLDTLLQEADFVCLILPLTEETHHLFGAEQFAKMKSSAIFINAGRGPV
VDEKALISALQKGEIHAAGLDVFEREPLPVDSPLLAMPNVVALPHIGSATHETRYNMAACAVDNLIAALQGNVDKNCVNP
QVAK
>Mature_324_residues
MKPSIILYKKLPDVLLQRLEEHFTVTQVSNLSPQTVEQHAEAFANAEGLLGSSEKVDDALLEKMPKLRATSTISVGYDNF
DVDALNARKVLLMHTPTVLTETVADTLMALILATSRRVVEVAERVKAGEWTASIGPDWFGSDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRQHPQAEERFNARYCDLDTLLQEADFVCLILPLTEETHHLFGAEQFAKMKSSAIFINAGRGPV
VDEKALISALQKGEIHAAGLDVFEREPLPVDSPLLAMPNVVALPHIGSATHETRYNMAACAVDNLIAALQGNVDKNCVNP
QVAK

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily

Homologues:

Organism=Homo sapiens, GI6912396, Length=291, Percent_Identity=41.2371134020619, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI23308577, Length=298, Percent_Identity=29.5302013422819, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI145580578, Length=270, Percent_Identity=32.5925925925926, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4557499, Length=270, Percent_Identity=32.5925925925926, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI145580575, Length=272, Percent_Identity=32.7205882352941, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI61743967, Length=268, Percent_Identity=32.089552238806, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI4557497, Length=268, Percent_Identity=32.089552238806, Blast_Score=124, Evalue=9e-29,
Organism=Escherichia coli, GI87082289, Length=323, Percent_Identity=88.2352941176471, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1789279, Length=293, Percent_Identity=29.0102389078498, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787645, Length=210, Percent_Identity=30, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081824, Length=285, Percent_Identity=27.0175438596491, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=249, Percent_Identity=33.7349397590361, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25147481, Length=300, Percent_Identity=28.3333333333333, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=277, Percent_Identity=34.2960288808664, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6320925, Length=288, Percent_Identity=29.8611111111111, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=260, Percent_Identity=28.8461538461538, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6325144, Length=178, Percent_Identity=35.9550561797753, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324964, Length=241, Percent_Identity=27.8008298755187, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321253, Length=278, Percent_Identity=25.1798561151079, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324980, Length=175, Percent_Identity=27.4285714285714, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45551003, Length=312, Percent_Identity=38.7820512820513, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24585514, Length=312, Percent_Identity=38.7820512820513, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574282, Length=312, Percent_Identity=38.7820512820513, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574284, Length=312, Percent_Identity=38.7820512820513, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI45552429, Length=312, Percent_Identity=38.7820512820513, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574286, Length=311, Percent_Identity=37.620578778135, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI28571528, Length=312, Percent_Identity=39.7435897435897, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24585516, Length=246, Percent_Identity=35.7723577235772, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24646446, Length=272, Percent_Identity=34.9264705882353, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24646448, Length=272, Percent_Identity=34.9264705882353, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24646452, Length=272, Percent_Identity=34.9264705882353, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24646450, Length=272, Percent_Identity=34.9264705882353, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI62472511, Length=272, Percent_Identity=34.9264705882353, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI19921140, Length=301, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GHRB_ESCF3 (B7LTG7)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002384629.1
- EnsemblBacteria:   EBESCT00000122804
- GeneID:   7120287
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_3554
- GeneTree:   EBGT00050000009079
- HOGENOM:   HBG731446
- ProtClustDB:   PRK15409
- BioCyc:   EFER585054:EFER_3554-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01667
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.79; =1.1.1.81

Molecular weight: Translated: 35509; Mature: 35509

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 237-237 ACT_SITE 266-266 ACT_SITE 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKPSIILYKKLPDVLLQRLEEHFTVTQVSNLSPQTVEQHAEAFANAEGLLGSSEKVDDAL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHCCCCCCCHHHHHH
LEKMPKLRATSTISVGYDNFDVDALNARKVLLMHTPTVLTETVADTLMALILATSRRVVE
HHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHH
VAERVKAGEWTASIGPDWFGSDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRQH
HHHHHHCCCCCCCCCCCCCCCCCCHHHHCEEECCHHHHHHHHHHHCCCCCCEEECCHHCC
PQAEERFNARYCDLDTLLQEADFVCLILPLTEETHHLFGAEQFAKMKSSAIFINAGRGPV
CCHHHHHCCCCCCHHHHHHHCCEEEEEEECCCCHHHHHCHHHHHHHCCCEEEEECCCCCC
VDEKALISALQKGEIHAAGLDVFEREPLPVDSPLLAMPNVVALPHIGSATHETRYNMAAC
CCHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCHHHCCCEEECCCCCCCCHHHHHHHHHH
AVDNLIAALQGNVDKNCVNPQVAK
HHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPSIILYKKLPDVLLQRLEEHFTVTQVSNLSPQTVEQHAEAFANAEGLLGSSEKVDDAL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHCCCCCCCHHHHHH
LEKMPKLRATSTISVGYDNFDVDALNARKVLLMHTPTVLTETVADTLMALILATSRRVVE
HHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHH
VAERVKAGEWTASIGPDWFGSDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRQH
HHHHHHCCCCCCCCCCCCCCCCCCHHHHCEEECCHHHHHHHHHHHCCCCCCEEECCHHCC
PQAEERFNARYCDLDTLLQEADFVCLILPLTEETHHLFGAEQFAKMKSSAIFINAGRGPV
CCHHHHHCCCCCCHHHHHHHCCEEEEEEECCCCHHHHHCHHHHHHHCCCEEEEECCCCCC
VDEKALISALQKGEIHAAGLDVFEREPLPVDSPLLAMPNVVALPHIGSATHETRYNMAAC
CCHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCHHHCCCEEECCCCCCCCHHHHHHHHHH
AVDNLIAALQGNVDKNCVNPQVAK
HHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA