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The map label for this gene is pcaK [H]

Identifier: 218550840

GI number: 218550840

Start: 3638759

End: 3640159

Strand: Direct

Name: pcaK [H]

Synonym: EFER_3556

Alternate gene names: 218550840

Gene position: 3638759-3640159 (Clockwise)

Preceding gene: 218550838

Following gene: 218550841

Centisome position: 79.3

GC content: 51.25

Gene sequence:

>1401_bases
TTGCCCGCCTTGCTTTGCAGGAATAATCGCATGACAAACAGAACAACTGATATTCAGGCATTTATTAATCAGCATCCTTT
TTCCCGCTACCAGTGGATGATCCTGGTACTGTGCTTTGTGACCGTCGCTATGGACGGTTTTGATACTGCAATTATCGGTT
TTATTGCTTCCGACCTGGTGCAGGAATGGGGCGTAGAGAAGTCAGCATTAGGCCCGGTAATGAGTGCTGCACTGGTCGGG
TTGGCTGTTGGCGCACTCACCGCAGGCCCGATGGCGGATCGTATTGGACGCAAGAAAGTACTGATTATGTCGATCCTGGT
ATTTGGTGGTTTTAGCTTATTAACCGCCTTTGCCAGTACGTTAAATCAACTTACTGCCCTACGCTTCTTAACCGGGCTGG
GGCTGGGTGCGGCAATGCCTAACGCGGCAACCTTAATGAGCGAGTATGCCCCGGAGCGTCGTCGCGCCTTACTGGTTAAC
CTGATGTTTGTAGGTTTTCCGATTGGCTCTTCGATGGGCGGATTTATTTCAGCCTGGCTTATCCCCCATTATGGCTGGCA
GAGCGTACTGATCCTCGGCGGTATAATGCCGTTGATTCTGGCTGTCGTTCTGATTTTCCTGTTACCGGAATCCGCACGTT
ACCTGGTGGTGAAAAATAAATCTCAGCAGCAAATCGGTACTATTTTACGCCGCATCGCCCCTGTTCCTGAGCAAACGTAT
TTTGTGTTGCGTGAGGCTGGGCTGGTAAAAGAGAAATCGGCGCTGGGTGTTATTTTCTCCCCACGTTACGCCGTGGGAAC
GGTGATGCTATGCCTGACCTATTTTATGGGCCTGTTGATTTTTTACCTGCTGACCAGCTGGCTGCCATTACTAATTCGTG
AAACAGGCGCGACCTTAAGTCAGGCGTCTATTATCACCGCGCTATTCCCTCTTGGTGGTGGGATCGGTGTCCTGCTGCTT
GGCGCGCTGATGGATAAACTGAACCCTAATAAAGTAGTCGCCGTTGGCTGGCTACTGACAGGCATCTTTGTTTGCCTGGT
AGGTTTCTCCACCAGTAACCTGGTATTAATGGGAGTGATGGTATTTATCGCCGGAACCATTATGAATGGCGCACAATCAT
CAATGCCTGCTCTGGCTGCTGGTTTTTACCCTACCCAGGGCCGTGCGACAGGCGTTGCCTGGATGTTAGGCCTGGGCCGC
TTTGGTGGGATCCTCGGCGCATTCAGTGGCGCGTTTTTAATGCAGGCCCAGCTGTCTTTCGAAACCATATTTACCTTCCT
GGCAATTCCGGCGCTGGTATCAGCACTGGCGCTCATGGTGAAATATTGGGTTGGTAAGCGCCAGTTACCTCTGACGCAAT
CCGCTACGGAAGATCTGAGTAGCGCAACGCAAAAAGCCTGA

Upstream 100 bases:

>100_bases
GAGTTTCAATGTTTTGATTAAATGAACGATTAACGGATAAAGGTTCGGTAATCGCACATTTGAATCACCAATGCGGTCGT
GAAGATAAGAACACCCTCCG

Downstream 100 bases:

>100_bases
TTATGTCGCCTGGCGAGAGTTGTTTCGCCAGGCGATTAAGATTTGCGCAAGTTTACATTGGTTATGCAGAAAAAATAAAT
TTGCGTATATCAATTTGTTA

Product: aromatic acid transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPALLCRNNRMTNRTTDIQAFINQHPFSRYQWMILVLCFVTVAMDGFDTAIIGFIASDLVQEWGVEKSALGPVMSAALVG
LAVGALTAGPMADRIGRKKVLIMSILVFGGFSLLTAFASTLNQLTALRFLTGLGLGAAMPNAATLMSEYAPERRRALLVN
LMFVGFPIGSSMGGFISAWLIPHYGWQSVLILGGIMPLILAVVLIFLLPESARYLVVKNKSQQQIGTILRRIAPVPEQTY
FVLREAGLVKEKSALGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGATLSQASIITALFPLGGGIGVLLL
GALMDKLNPNKVVAVGWLLTGIFVCLVGFSTSNLVLMGVMVFIAGTIMNGAQSSMPALAAGFYPTQGRATGVAWMLGLGR
FGGILGAFSGAFLMQAQLSFETIFTFLAIPALVSALALMVKYWVGKRQLPLTQSATEDLSSATQKA

Sequences:

>Translated_466_residues
MPALLCRNNRMTNRTTDIQAFINQHPFSRYQWMILVLCFVTVAMDGFDTAIIGFIASDLVQEWGVEKSALGPVMSAALVG
LAVGALTAGPMADRIGRKKVLIMSILVFGGFSLLTAFASTLNQLTALRFLTGLGLGAAMPNAATLMSEYAPERRRALLVN
LMFVGFPIGSSMGGFISAWLIPHYGWQSVLILGGIMPLILAVVLIFLLPESARYLVVKNKSQQQIGTILRRIAPVPEQTY
FVLREAGLVKEKSALGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGATLSQASIITALFPLGGGIGVLLL
GALMDKLNPNKVVAVGWLLTGIFVCLVGFSTSNLVLMGVMVFIAGTIMNGAQSSMPALAAGFYPTQGRATGVAWMLGLGR
FGGILGAFSGAFLMQAQLSFETIFTFLAIPALVSALALMVKYWVGKRQLPLTQSATEDLSSATQKA
>Mature_465_residues
PALLCRNNRMTNRTTDIQAFINQHPFSRYQWMILVLCFVTVAMDGFDTAIIGFIASDLVQEWGVEKSALGPVMSAALVGL
AVGALTAGPMADRIGRKKVLIMSILVFGGFSLLTAFASTLNQLTALRFLTGLGLGAAMPNAATLMSEYAPERRRALLVNL
MFVGFPIGSSMGGFISAWLIPHYGWQSVLILGGIMPLILAVVLIFLLPESARYLVVKNKSQQQIGTILRRIAPVPEQTYF
VLREAGLVKEKSALGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGATLSQASIITALFPLGGGIGVLLLG
ALMDKLNPNKVVAVGWLLTGIFVCLVGFSTSNLVLMGVMVFIAGTIMNGAQSSMPALAAGFYPTQGRATGVAWMLGLGRF
GGILGAFSGAFLMQAQLSFETIFTFLAIPALVSALALMVKYWVGKRQLPLTQSATEDLSSATQKA

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=440, Percent_Identity=26.3636363636364, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI7662270, Length=347, Percent_Identity=22.1902017291066, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI216548223, Length=350, Percent_Identity=22.2857142857143, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI262399383, Length=262, Percent_Identity=23.6641221374046, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI213021148, Length=465, Percent_Identity=22.5806451612903, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI73695465, Length=236, Percent_Identity=24.1525423728814, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI154350196, Length=424, Percent_Identity=24.0566037735849, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI166064021, Length=340, Percent_Identity=25.8823529411765, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI87081723, Length=390, Percent_Identity=37.6923076923077, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI87082231, Length=450, Percent_Identity=23.3333333333333, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082404, Length=367, Percent_Identity=23.1607629427793, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI87082159, Length=391, Percent_Identity=24.2966751918159, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786229, Length=205, Percent_Identity=26.3414634146341, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788074, Length=388, Percent_Identity=23.4536082474227, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789152, Length=455, Percent_Identity=20.6593406593407, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082320, Length=315, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789207, Length=265, Percent_Identity=25.6603773584906, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788068, Length=466, Percent_Identity=22.7467811158798, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789312, Length=236, Percent_Identity=27.5423728813559, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI48994889, Length=186, Percent_Identity=25.8064516129032, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=453, Percent_Identity=21.8543046357616, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17565978, Length=233, Percent_Identity=28.3261802575107, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24640198, Length=452, Percent_Identity=23.4513274336283, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640196, Length=452, Percent_Identity=23.4513274336283, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640200, Length=452, Percent_Identity=23.4513274336283, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922874, Length=417, Percent_Identity=23.2613908872902, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24646916, Length=366, Percent_Identity=26.5027322404372, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221330907, Length=373, Percent_Identity=25.201072386059, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49988; Mature: 49856

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALLCRNNRMTNRTTDIQAFINQHPFSRYQWMILVLCFVTVAMDGFDTAIIGFIASDLV
CCCEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QEWGVEKSALGPVMSAALVGLAVGALTAGPMADRIGRKKVLIMSILVFGGFSLLTAFAST
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LNQLTALRFLTGLGLGAAMPNAATLMSEYAPERRRALLVNLMFVGFPIGSSMGGFISAWL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IPHYGWQSVLILGGIMPLILAVVLIFLLPESARYLVVKNKSQQQIGTILRRIAPVPEQTY
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHH
FVLREAGLVKEKSALGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGATLS
HHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QASIITALFPLGGGIGVLLLGALMDKLNPNKVVAVGWLLTGIFVCLVGFSTSNLVLMGVM
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHH
VFIAGTIMNGAQSSMPALAAGFYPTQGRATGVAWMLGLGRFGGILGAFSGAFLMQAQLSF
HHHHHHHHCCCHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIFTFLAIPALVSALALMVKYWVGKRQLPLTQSATEDLSSATQKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PALLCRNNRMTNRTTDIQAFINQHPFSRYQWMILVLCFVTVAMDGFDTAIIGFIASDLV
CCEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QEWGVEKSALGPVMSAALVGLAVGALTAGPMADRIGRKKVLIMSILVFGGFSLLTAFAST
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LNQLTALRFLTGLGLGAAMPNAATLMSEYAPERRRALLVNLMFVGFPIGSSMGGFISAWL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IPHYGWQSVLILGGIMPLILAVVLIFLLPESARYLVVKNKSQQQIGTILRRIAPVPEQTY
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHH
FVLREAGLVKEKSALGVIFSPRYAVGTVMLCLTYFMGLLIFYLLTSWLPLLIRETGATLS
HHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QASIITALFPLGGGIGVLLLGALMDKLNPNKVVAVGWLLTGIFVCLVGFSTSNLVLMGVM
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHH
VFIAGTIMNGAQSSMPALAAGFYPTQGRATGVAWMLGLGRFGGILGAFSGAFLMQAQLSF
HHHHHHHHCCCHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIFTFLAIPALVSALALMVKYWVGKRQLPLTQSATEDLSSATQKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]