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The map label for this gene is yrbE [H]

Identifier: 218550477

GI number: 218550477

Start: 3257885

End: 3258667

Strand: Reverse

Name: yrbE [H]

Synonym: EFER_3171

Alternate gene names: 218550477

Gene position: 3258667-3257885 (Counterclockwise)

Preceding gene: 218550478

Following gene: 218550476

Centisome position: 71.01

GC content: 52.36

Gene sequence:

>783_bases
ATGCTGTTAAATGCACTGGCTGCGTTCGGGCGTAAGGGGATAACAACCCTCAGAACGTTTGGACGCGCCGGGTTAATGTT
ATTCAATGCGATTGTCGGTAAGCCGGAGTTTCGTAAACATACTCCGCTACTGATACGGCAAATTTATAATGTCGGCGTGC
TTTCGATGCTGATCATTATTGTTTCAGGCGTCTTCATCGGTATGGTGCTGGGGCTGCAGGGCTACCTTGTCCTCACCACT
TACAGTGCCGAAACCAGCCTCGGCATGTTGGTGGCGCTGTCTCTGCTTCGCGAGCTGGGGCCTGTTGTTGCAGCACTGCT
TTTTGCTGGTCGTGCCGGTTCGGCACTTACCGCTGAAATTGGCTTGATGCGTGCCACTGAACAGCTTTCCAGTATGGAAA
TGATGGCAGTCGATCCGCTCCGGCGGGTGATTTCACCGCGTTTTTGGGCTGGAGTAATCTCCTTGCCATTGCTGACCATT
ATTTTTGTCGCCGTGGGGATCTGGGGCGGTTCACTGGTTGGCGTCAGCTGGAAGGGGATCGATGCAGGTTTCTTCTGGTC
GGCGATGCAAAACGCCGTTGACTGGCGTCTGGATTTAGTTAACTGCCTGATTAAAAGCGTTGTTTTTGCCATCACTGTAA
CGTGGATCGCGTTATTCAATGGCTATGATGCGATTCCGACTTCTGCCGGGATCAGTCGGGCGACTACACGTACTGTTGTT
CACGCATCTCTGGCAGTCCTGGGGCTGGATTTTGTGCTGACTGCACTGATGTTTGGGAATTGA

Upstream 100 bases:

>100_bases
CAGTTCCTGGATGGTATTGCTGACGGTCCTGTTCCGTTCCGTTATCCTGCGGGCGATTATCATTCTGATTTACTACCAGT
GACAGGGAGTTGAACCGCTC

Downstream 100 bases:

>100_bases
GTTCATGCAAACGAAAAAAAATGAAATTTGGGTCGGTTTTTTTCTGCTGGTGGCGTTACTGGCAGCGCTATTTGTCTGCC
TGAAAGCAGCCAATGTCACC

Product: toluene ABC transporter membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLLNALAAFGRKGITTLRTFGRAGLMLFNAIVGKPEFRKHTPLLIRQIYNVGVLSMLIIIVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTI
IFVAVGIWGGSLVGVSWKGIDAGFFWSAMQNAVDWRLDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
HASLAVLGLDFVLTALMFGN

Sequences:

>Translated_260_residues
MLLNALAAFGRKGITTLRTFGRAGLMLFNAIVGKPEFRKHTPLLIRQIYNVGVLSMLIIIVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTI
IFVAVGIWGGSLVGVSWKGIDAGFFWSAMQNAVDWRLDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
HASLAVLGLDFVLTALMFGN
>Mature_260_residues
MLLNALAAFGRKGITTLRTFGRAGLMLFNAIVGKPEFRKHTPLLIRQIYNVGVLSMLIIIVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTI
IFVAVGIWGGSLVGVSWKGIDAGFFWSAMQNAVDWRLDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
HASLAVLGLDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=260, Percent_Identity=94.6153846153846, Blast_Score=493, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27880; Mature: 27880

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNALAAFGRKGITTLRTFGRAGLMLFNAIVGKPEFRKHTPLLIRQIYNVGVLSMLIII
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEI
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTIIFVAVGIWGGSLVGVSWKGI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DAGFFWSAMQNAVDWRLDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
HASLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLNALAAFGRKGITTLRTFGRAGLMLFNAIVGKPEFRKHTPLLIRQIYNVGVLSMLIII
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEI
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTIIFVAVGIWGGSLVGVSWKGI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DAGFFWSAMQNAVDWRLDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
HASLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]