Click here to switch to the map view.

The map label for this gene is yrbD [H]

Identifier: 218550476

GI number: 218550476

Start: 3257329

End: 3257895

Strand: Reverse

Name: yrbD [H]

Synonym: EFER_3170

Alternate gene names: 218550476

Gene position: 3257895-3257329 (Counterclockwise)

Preceding gene: 218550477

Following gene: 218550475

Centisome position: 71.0

GC content: 47.97

Gene sequence:

>567_bases
TTGGGAATTGAGTTCATGCAAACGAAAAAAAATGAAATTTGGGTCGGTTTTTTTCTGCTGGTGGCGTTACTGGCAGCGCT
ATTTGTCTGCCTGAAAGCAGCCAATGTCACCTCTATACGTACTGAGCCGACTTATACCCTTTATGCGACTTTCGATAACA
TCGGTGGCCTGAAAGTACGCTCGCCAGTACGTATTGGTGGTGTTGTAGTGGGGCGCGTGTCTGATATCACTCTCGATCCG
CAAACCTATTTGCCGCGTGTGACCCTGGACATTGAACAGCGCTATAACCATATACCAGATACCAGTTCTCTTAGTATCCG
TACGTCCGGCTTACTTGGTGAGCAATATCTGGCTTTAAACGTCGGTTTTGAAGATCCAGAGTTGGGTACGTCTATCCTGA
AAGATGGTGGCACCATTCAGGACACCAAGTCTGCGATGGTACTGGAAGACCTTATTGGTCAGTTCCTGTATAGCGGCAAG
GGCGAAGAGAATAAGAATTCTGGCGATGCGCCAGCGCCAGATGAAGGCAATAATCAAGTCAATGAGCCTGCGGGCGCAAC
GAAATAA

Upstream 100 bases:

>100_bases
ATTCCGACTTCTGCCGGGATCAGTCGGGCGACTACACGTACTGTTGTTCACGCATCTCTGGCAGTCCTGGGGCTGGATTT
TGTGCTGACTGCACTGATGT

Downstream 100 bases:

>100_bases
TCTCAGGAGAATCGACACATGTTCAAACGCTTAATGATGATTGCTCTGCTGGTGATTGCTCCGCTGTCTGCGGCAACCGC
AGCGGATCAAACAAATCCAT

Product: toluene ABC transporter membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MGIEFMQTKKNEIWVGFFLLVALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKVRSPVRIGGVVVGRVSDITLDP
QTYLPRVTLDIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTSILKDGGTIQDTKSAMVLEDLIGQFLYSGK
GEENKNSGDAPAPDEGNNQVNEPAGATK

Sequences:

>Translated_188_residues
MGIEFMQTKKNEIWVGFFLLVALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKVRSPVRIGGVVVGRVSDITLDP
QTYLPRVTLDIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTSILKDGGTIQDTKSAMVLEDLIGQFLYSGK
GEENKNSGDAPAPDEGNNQVNEPAGATK
>Mature_187_residues
GIEFMQTKKNEIWVGFFLLVALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKVRSPVRIGGVVVGRVSDITLDPQ
TYLPRVTLDIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTSILKDGGTIQDTKSAMVLEDLIGQFLYSGKG
EENKNSGDAPAPDEGNNQVNEPAGATK

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family [H]

Homologues:

Organism=Escherichia coli, GI1789584, Length=183, Percent_Identity=88.5245901639344, Blast_Score=316, Evalue=7e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003399 [H]

Pfam domain/function: PF02470 MCE [H]

EC number: NA

Molecular weight: Translated: 20355; Mature: 20224

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIEFMQTKKNEIWVGFFLLVALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKVR
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCEEEC
SPVRIGGVVVGRVSDITLDPQTYLPRVTLDIEQRYNHIPDTSSLSIRTSGLLGEQYLALN
CCEEECCEEEEEEEEEEECCHHHCCEEEEEHHHHCCCCCCCCCEEEEECCCCCCEEEEEE
VGFEDPELGTSILKDGGTIQDTKSAMVLEDLIGQFLYSGKGEENKNSGDAPAPDEGNNQV
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
NEPAGATK
CCCCCCCC
>Mature Secondary Structure 
GIEFMQTKKNEIWVGFFLLVALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKVR
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCEEEC
SPVRIGGVVVGRVSDITLDPQTYLPRVTLDIEQRYNHIPDTSSLSIRTSGLLGEQYLALN
CCEEECCEEEEEEEEEEECCHHHCCEEEEEHHHHCCCCCCCCCEEEEECCCCCCEEEEEE
VGFEDPELGTSILKDGGTIQDTKSAMVLEDLIGQFLYSGKGEENKNSGDAPAPDEGNNQV
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
NEPAGATK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]