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The map label for this gene is ispB [H]

Identifier: 218550470

GI number: 218550470

Start: 3253140

End: 3254111

Strand: Direct

Name: ispB [H]

Synonym: EFER_3164

Alternate gene names: 218550470

Gene position: 3253140-3254111 (Clockwise)

Preceding gene: 218550465

Following gene: 218550471

Centisome position: 70.89

GC content: 49.28

Gene sequence:

>972_bases
ATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTCAATGCGGCAATCCTTGAGCAGCTTAATTC
CGACGTCCAACTGATCAATCAGTTAGGTTATTACATCGTTAGTGGTGGCGGAAAACGTATTCGTCCGATGATCGCCGTAC
TCGCTGCGCGGGCCGTTGGTTATCAGGGCAATGCACATGTGACAATTGCGGCCTTAATCGAGTTTATCCACACTGCCACT
CTGCTACACGATGATGTTGTGGATGAATCAGATATGCGCCGTGGGAAAGCCACAGCAAACGCTGCATTTGGTAATGCTGC
CAGCGTACTGGTTGGTGATTTTATTTATACCCGCGCTTTCCAGATGATGACCAGCCTTGGTTCACTCAAAGTGCTGGAAG
TGATGTCTGAGGCTGTTAACGTCATTGCTGAAGGTGAAGTTCTGCAATTAATGAACGTTAATGATCCGAACATTACTGAA
GAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGG
CTGTACGCCGGAGGAGGAGCAAGGCTTGCAGGATTATGGGCGTTATCTCGGCACCGCTTTCCAGTTAATCGACGACCTGT
TGGATTATAGTGCTGACGGTGAACAATTAGGCAAAAACGTGGGCGATGATCTTAATGAGGGTAAACCCACCTTGCCATTA
CTCCACGCGATGCGTCACGGTACACCGGAGCAAGCGCAGATGATTCGTAATGCGATTGAGCACGGTAATGGCCGACATCT
TCTGGAACCTGTTCTGGAAGCAATGAACGCTTGTGGTTCACTGGAATGGACTCGTCAGCGTGCCGAAGAAGAAGCGGATA
AAGCCATTAAAGCTCTGCAGGTTCTTCCTGACACACCATGGCGTGAAGCCTTGATCGGTCTGGCACATATCGCGGTACAA
CGCGATCATTAA

Upstream 100 bases:

>100_bases
GTCGTTTTTTCAGTACAATCAGTCTATATTTCTAACCCTAAACCCTAAGTTACCTTCACTCAATCTTGAGGTAATCGGGA
CAAAAAGCCCGGCTTTTGCG

Downstream 100 bases:

>100_bases
TCCCTGCAGTTATATTTCAATTAATTCTATCCCGCCTTGTGCGGGATAATTCCAGTAAGTTCTTTAAACATTACAATCTT
ACGTTTGTTCTTTCCCAATA

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYQGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPNITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEQGLQDYGRYLGTAFQLIDDLLDYSADGEQLGKNVGDDLNEGKPTLPL
LHAMRHGTPEQAQMIRNAIEHGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIKALQVLPDTPWREALIGLAHIAVQ
RDH

Sequences:

>Translated_323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYQGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPNITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEQGLQDYGRYLGTAFQLIDDLLDYSADGEQLGKNVGDDLNEGKPTLPL
LHAMRHGTPEQAQMIRNAIEHGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIKALQVLPDTPWREALIGLAHIAVQ
RDH
>Mature_323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYQGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPNITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEQGLQDYGRYLGTAFQLIDDLLDYSADGEQLGKNVGDDLNEGKPTLPL
LHAMRHGTPEQAQMIRNAIEHGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIKALQVLPDTPWREALIGLAHIAVQ
RDH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=31.055900621118, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI4758430, Length=285, Percent_Identity=24.9122807017544, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI83700220, Length=285, Percent_Identity=24.9122807017544, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=97.5155279503106, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1786623, Length=229, Percent_Identity=29.6943231441048, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=343, Percent_Identity=33.5276967930029, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=298, Percent_Identity=33.8926174496644, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6325188, Length=227, Percent_Identity=25.1101321585903, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=31.8791946308725, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI281365769, Length=280, Percent_Identity=22.1428571428571, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24660002, Length=280, Percent_Identity=22.1428571428571, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35268; Mature: 35268

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
YQGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPNITEENYMRVIYSKTARLFEAAAQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
CSGILAGCTPEEEQGLQDYGRYLGTAFQLIDDLLDYSADGEQLGKNVGDDLNEGKPTLPL
HCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMRHGTPEQAQMIRNAIEHGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIKALQ
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VLPDTPWREALIGLAHIAVQRDH
HCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
YQGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPNITEENYMRVIYSKTARLFEAAAQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
CSGILAGCTPEEEQGLQDYGRYLGTAFQLIDDLLDYSADGEQLGKNVGDDLNEGKPTLPL
HCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMRHGTPEQAQMIRNAIEHGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIKALQ
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VLPDTPWREALIGLAHIAVQRDH
HCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]