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The map label for this gene is 218550409

Identifier: 218550409

GI number: 218550409

Start: 3191441

End: 3193015

Strand: Reverse

Name: 218550409

Synonym: EFER_3101

Alternate gene names: NA

Gene position: 3193015-3191441 (Counterclockwise)

Preceding gene: 218550410

Following gene: 218550408

Centisome position: 69.58

GC content: 43.49

Gene sequence:

>1575_bases
ATGATGGCAACTATTTTTAAAAGAACACCCGAACAATTGTCTGAAAGGCGGATTGATCCTTGGCACTATCAACCGCGATT
TGAAAGACAAATTAATAAGATAAAAGACAACCTAGGTGCTTTGCCCCTGGAAGCGGTAATTGATTCAAAGCGCGGGGTGG
CTAGTGGCGCAACACCTTTAGGAGCAAATTATTTAGAAAAAGGCCGTGTTAAGTTTTATCGTACTTCTGAAGTTGAAGAA
ATGTTCTTGCAAGCTGAAGAAGCTGTTTTTATTAACGATGAAGATGATGAAAAATTAAAGCGCAGCCGATTAGTGGATGG
CGACGTTTTACTTACTATTACTGGTGCAAAATTTGGTAAATCGGCTGTTGTGTCAGCTAAACATCTACCGGCCAACATTA
GTCAGCACTCAGTCAGATTCAAGCCCGATCCCAAAAAAGTTGACGCCTATTTCTTGGTGGCATATTTGAATAGCAAAACC
GGACAAGTAGCGATCTGGAAGGAGGCATATGGCGCAACTCGCCCTGCAATTGACTTCCCAAGTGTCCGCTCGCTAGCAGT
TCCTAAAGTTTTACCTCTAGCCCAAAAATACATCGGGGATAAAGTTCGCCAAGCCGAGCAACTGCGCGTTTGGGCGAAGA
GGCTCAATTCGGTTTTACAGTCACAAATTCACTCTGTATTTAAAGGTGATCCTAAGCCAGAAAAAAGAATTGGTAAGGTT
ATATCAATTCAACAACTATCCTCCCTGAGGCTGGAGGCTGAATACTATGGTGATTTAGAGCTTTGGGCGGAACTCGAAAT
TAAGAACTCTCCTTTTCCGAATAAACCACTCGGGGAGTTATCTTCAAGAATTAAAGATGGTCCTGGCGGATGGGCGGTGT
CTACAAGCGACTATAGACCATCAGGCATTCCAGTTATAAGAAGTGTTAATTTAATCGACGGTCGCTGCGAATTAGAGGAC
TGCGTTTTCATCTCTAAGGAAAAGCATAATGATCTGAGATCGCATCAAGTAAAGCCTGGTGGTCTTTTGCTTTCTGTTCG
AGGAACAATCGGCCGCGCTGCTGTATTTGACAGCGAGAAATACAGCACTGCTAGCCTGAATGCGGCGGTTGTAACTATTG
ACTGCAAACCAACTATAAACCCGTACTATTTAGCAGCATTTTTAAACTCAGAAGTTGGAAGGATTCAATCGAACCGAATT
GCAAACGGCGCAGTTCAATTAAATATGAATTTAAAAGAAACAGCATCAAACTTAATTGTTATACCGCCTATTAATTTACA
AGAAACTATCGCGGCAACTTTCCTATCCAAAAACCGAGCTATTATCTTAGCTAATTTATTAATTCAGTCCGCCAAAACCC
TGGTCGAAGCCCTCATCGAAGGCCAACTGACTGAGCAACAATTAATCCAGGCCCAACAGGCCCTGGAAGATGGCGACAAC
AGCTTAGATCAGGCGATTTTAAGTAAACTGAGCGCTGAGGGGTATGCCATAGAAGGCGCCACACCGCTATTTGGCGATGT
GGATGAGTTATACAGTTTGCTGGAAGAGGCGGGGCAAGCGGAGGCTGAAGAGTAA

Upstream 100 bases:

>100_bases
CTGAAACCTTGGGCTACGTGGTGAAAAACAATATCGAAGACTATAACGCCGGTGTCGCTAACGATCTGGATAAGATTGTG
TCGGCTTATAAGCGGGGGGA

Downstream 100 bases:

>100_bases
AGATGGAGACAAGGGGCATGGACAGCATTACAACCAGGCAAAATAACGACCCCGTAAATAGCGAAGTGGCACTTGACCTC
TGCCTGCAGCTTTGGCGGCA

Product: restriction modification system DNA specificity domain

Products: NA

Alternate protein names: Restriction System DNA Specificity Domain Protein; DNA Methylase-Type I Restriction- System; Specificity Determinant HsdS; Type I Restriction System N-6 DNA Methylase; Type I Restriction- System; Type I Restriction- S Subunit EcoA Family Protein; Type I Restriction StySPI Specificity Protein; Type I Restriction DNA Specificity Domain Protein; N-6 DNA Methylase; Restriction And System Specificity Protein; Restriction System DNA Specificity Domain

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MMATIFKRTPEQLSERRIDPWHYQPRFERQINKIKDNLGALPLEAVIDSKRGVASGATPLGANYLEKGRVKFYRTSEVEE
MFLQAEEAVFINDEDDEKLKRSRLVDGDVLLTITGAKFGKSAVVSAKHLPANISQHSVRFKPDPKKVDAYFLVAYLNSKT
GQVAIWKEAYGATRPAIDFPSVRSLAVPKVLPLAQKYIGDKVRQAEQLRVWAKRLNSVLQSQIHSVFKGDPKPEKRIGKV
ISIQQLSSLRLEAEYYGDLELWAELEIKNSPFPNKPLGELSSRIKDGPGGWAVSTSDYRPSGIPVIRSVNLIDGRCELED
CVFISKEKHNDLRSHQVKPGGLLLSVRGTIGRAAVFDSEKYSTASLNAAVVTIDCKPTINPYYLAAFLNSEVGRIQSNRI
ANGAVQLNMNLKETASNLIVIPPINLQETIAATFLSKNRAIILANLLIQSAKTLVEALIEGQLTEQQLIQAQQALEDGDN
SLDQAILSKLSAEGYAIEGATPLFGDVDELYSLLEEAGQAEAEE

Sequences:

>Translated_524_residues
MMATIFKRTPEQLSERRIDPWHYQPRFERQINKIKDNLGALPLEAVIDSKRGVASGATPLGANYLEKGRVKFYRTSEVEE
MFLQAEEAVFINDEDDEKLKRSRLVDGDVLLTITGAKFGKSAVVSAKHLPANISQHSVRFKPDPKKVDAYFLVAYLNSKT
GQVAIWKEAYGATRPAIDFPSVRSLAVPKVLPLAQKYIGDKVRQAEQLRVWAKRLNSVLQSQIHSVFKGDPKPEKRIGKV
ISIQQLSSLRLEAEYYGDLELWAELEIKNSPFPNKPLGELSSRIKDGPGGWAVSTSDYRPSGIPVIRSVNLIDGRCELED
CVFISKEKHNDLRSHQVKPGGLLLSVRGTIGRAAVFDSEKYSTASLNAAVVTIDCKPTINPYYLAAFLNSEVGRIQSNRI
ANGAVQLNMNLKETASNLIVIPPINLQETIAATFLSKNRAIILANLLIQSAKTLVEALIEGQLTEQQLIQAQQALEDGDN
SLDQAILSKLSAEGYAIEGATPLFGDVDELYSLLEEAGQAEAEE
>Mature_524_residues
MMATIFKRTPEQLSERRIDPWHYQPRFERQINKIKDNLGALPLEAVIDSKRGVASGATPLGANYLEKGRVKFYRTSEVEE
MFLQAEEAVFINDEDDEKLKRSRLVDGDVLLTITGAKFGKSAVVSAKHLPANISQHSVRFKPDPKKVDAYFLVAYLNSKT
GQVAIWKEAYGATRPAIDFPSVRSLAVPKVLPLAQKYIGDKVRQAEQLRVWAKRLNSVLQSQIHSVFKGDPKPEKRIGKV
ISIQQLSSLRLEAEYYGDLELWAELEIKNSPFPNKPLGELSSRIKDGPGGWAVSTSDYRPSGIPVIRSVNLIDGRCELED
CVFISKEKHNDLRSHQVKPGGLLLSVRGTIGRAAVFDSEKYSTASLNAAVVTIDCKPTINPYYLAAFLNSEVGRIQSNRI
ANGAVQLNMNLKETASNLIVIPPINLQETIAATFLSKNRAIILANLLIQSAKTLVEALIEGQLTEQQLIQAQQALEDGDN
SLDQAILSKLSAEGYAIEGATPLFGDVDELYSLLEEAGQAEAEE

Specific function: Unknown

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 57925; Mature: 57925

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMATIFKRTPEQLSERRIDPWHYQPRFERQINKIKDNLGALPLEAVIDSKRGVASGATPL
CCCHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCC
GANYLEKGRVKFYRTSEVEEMFLQAEEAVFINDEDDEKLKRSRLVDGDVLLTITGAKFGK
CCHHHHCCCEEEEECHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCC
SAVVSAKHLPANISQHSVRFKPDPKKVDAYFLVAYLNSKTGQVAIWKEAYGATRPAIDFP
HHEEEHHCCCCCCCCCCEEECCCCCHHCCEEEEEEECCCCCEEEEEHHHCCCCCCCCCCC
SVRSLAVPKVLPLAQKYIGDKVRQAEQLRVWAKRLNSVLQSQIHSVFKGDPKPEKRIGKV
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ISIQQLSSLRLEAEYYGDLELWAELEIKNSPFPNKPLGELSSRIKDGPGGWAVSTSDYRP
HHHHHHHHHEEEHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCC
SGIPVIRSVNLIDGRCELEDCVFISKEKHNDLRSHQVKPGGLLLSVRGTIGRAAVFDSEK
CCCCEEEEEEEECCCCCCCHHEEEECHHCCCHHHCCCCCCCEEEEECCCCCEEEEECCCC
YSTASLNAAVVTIDCKPTINPYYLAAFLNSEVGRIQSNRIANGAVQLNMNLKETASNLIV
CCEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHCCEEE
IPPINLQETIAATFLSKNRAIILANLLIQSAKTLVEALIEGQLTEQQLIQAQQALEDGDN
ECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
SLDQAILSKLSAEGYAIEGATPLFGDVDELYSLLEEAGQAEAEE
HHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMATIFKRTPEQLSERRIDPWHYQPRFERQINKIKDNLGALPLEAVIDSKRGVASGATPL
CCCHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCC
GANYLEKGRVKFYRTSEVEEMFLQAEEAVFINDEDDEKLKRSRLVDGDVLLTITGAKFGK
CCHHHHCCCEEEEECHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCC
SAVVSAKHLPANISQHSVRFKPDPKKVDAYFLVAYLNSKTGQVAIWKEAYGATRPAIDFP
HHEEEHHCCCCCCCCCCEEECCCCCHHCCEEEEEEECCCCCEEEEEHHHCCCCCCCCCCC
SVRSLAVPKVLPLAQKYIGDKVRQAEQLRVWAKRLNSVLQSQIHSVFKGDPKPEKRIGKV
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ISIQQLSSLRLEAEYYGDLELWAELEIKNSPFPNKPLGELSSRIKDGPGGWAVSTSDYRP
HHHHHHHHHEEEHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCC
SGIPVIRSVNLIDGRCELEDCVFISKEKHNDLRSHQVKPGGLLLSVRGTIGRAAVFDSEK
CCCCEEEEEEEECCCCCCCHHEEEECHHCCCHHHCCCCCCCEEEEECCCCCEEEEECCCC
YSTASLNAAVVTIDCKPTINPYYLAAFLNSEVGRIQSNRIANGAVQLNMNLKETASNLIV
CCEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHCCEEE
IPPINLQETIAATFLSKNRAIILANLLIQSAKTLVEALIEGQLTEQQLIQAQQALEDGDN
ECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
SLDQAILSKLSAEGYAIEGATPLFGDVDELYSLLEEAGQAEAEE
HHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA