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The map label for this gene is mamIM [H]

Identifier: 218550410

GI number: 218550410

Start: 3193012

End: 3195159

Strand: Reverse

Name: mamIM [H]

Synonym: EFER_3102

Alternate gene names: 218550410

Gene position: 3195159-3193012 (Counterclockwise)

Preceding gene: 218550411

Following gene: 218550409

Centisome position: 69.63

GC content: 47.63

Gene sequence:

>2148_bases
ATGGCAAAAGAAGCAAAAAAACAAGACCCGCAACAGCAGCTAGAAGCACTGTTCAATAAACTGGTCAAACAGCACTCCGA
GTTTACTCAGCCATTGGTCGAATTACCCGAGCATGAATTCGCCAAGGCTGGTCTCATTCAAGGCCTGGTTGAAGGCAACT
TAACGCCACCAGTCATGTTGATTGCGCTGACAGAAGGCCATTGGGAATACGCCGCTGACACTGCAAAGTTTGATGATTTA
GCCTATCAGCTGTTAAGTGATATCGAAGGTGACTGGCCAGTATATGCCGTTGTGGACGATGGCTTGAACCAACGTATTTT
GGCCTTGTTCGGTGCCGATGGATCTGACGGTACCCACGGTGCAGATACCCTGCCTGGTCTGGAAGAATTACGTAGCTTTG
ATCGCATGGAGCGTGATCCAACCTTTCGCTGGTCGATGCGTGTTTATACACGCTTAATGCAGCGATTTGATGCCTTCCAT
GAAAATGTTTATCGCGTTACCAAAGACAAGGTAAACGACAAGAACGACATTATTGAGGAAGTGGCTAAGCTTCTGTTTTT
AGAAACTTTCCGCCTGCATCACGATGAAGACCTTACCTTTAAAGATGATGAGGGAAATACCCTGCGTTTTCGTGATGTCT
TTGATTGGCAATATGTGGAGAGCCACGGCGATAAAGCCGTTGCCCAAATCAAAGCCGCCTTTGAGGAATTTAAAAATCAC
GAAAACTACGTTGTGATCTCAGATGATGGCTCACGTAACCCCATCTTTAGTAAAGAAACACACCTGCGACTGTCTGTTGC
GAAAAACTATCAGGATTTGCTGGAAGCAATACAAAACCTGGGTCCGGTAAAAACCAACGACGGTAAAATCGCTAAAGAAC
ACGGCACGTTGGCTGATGTGTCAGGAGATTTACTCGGCAGAGTGTTTGATGTATTCCTACGCGCTAACTTTGAATCCAAA
GGTGGCCTTGGCGTTTATTTAACACCAAACCCAGTAAAACAGGCCATGCTTGAAATTGCTATGCACGACATCGACGATGA
CGATGAAATGCGATCGCGCTTAGCCAATGGCGATTTTCGTTTCTGCGACCCCACCTGTGGCTCCTTTGGTTTTGGCTCGG
TGGCGTTAAGCCAGATTGATAAATGGATCGATTTTAAGCTGGTGCTGGCCGACGATAAAAAAGAATCATTGAAACAAAAA
TTACGCGACTGTGCATTTACCGGTGCCGATGCTGCACCACGCATGGTAATGCTGGCCCGCGTGAATATGGCATTGCAAGG
TGCCCCCAAAGCGCAGATTTTTTATACCGACAACTCACTCACTACTAACGCTCTGAAGCCGAATAGCTTTGACCTGATTT
GCACCAACCCGCCGTTCGGTACGCCTAAATTTGATAAAGGCAAAAATGGTCAAAACTCAAAAGCCAATTACGAAGCCAAT
ATGGAGCAAGTGCTCGGTGGTTTTCGTCCGACCAAAAAAGTCGTTGATAGCTACAACGCCTGGTATGACCACGTAAAAAT
GAAGTGGGAGGATATCGGTGATTTAGAGCTGGATGAAAACGGCGAGCCTAAATGGGCGGGCTATCGTACCGATTTGCGCA
GAACGGGCGGGACTGATAAAAAGCCGTTTTACAGCCTGCAGCCTACAACCGCAGGGCTCGCGCTGGGCAGCAAGCCCGAT
AGCAAGGGCAACTGGCAACCGGTAGGTGCCACTATTGACCCAGCAGTACTTTTTATCGACCGCTGCCTGCAACTGCTTAA
ACCCGGAGGCCGTCTGTTAATAGTGCTGCCCGATGGTATTTTATGTAACTCCGGCGACCGTTATGTCCGCGAGTACATTA
TGGGGAAAAAAGACGAAAAAACCGGCGAGTTTGTTGGCGGTAAAGCCATTGTAAAAGCAGTAATTTCATTGCCGAGCGAC
TGCTTTAAGCTCTCAGGCACAGGCGCCAAAACCTCGATTTTGTATTTGCAAAAACGCCATGCTAACCCCAAACAGCCAGA
GCAATTTTTGCCCGAGCCACAAACTGACGTGTTTATGGCAGTAGCTGAAACCTTGGGCTACGTGGTGAAAAACAATATCG
AAGACTATAACGCCGGTGTCGCTAACGATCTGGATAAGATTGTGTCGGCTTATAAGCGGGGGGAATGA

Upstream 100 bases:

>100_bases
AGCACGCGCAGATAGAGATTAAAGGCCCCTTGCTGGTACTGATAAATTAGCCGGCATTTGTAGTAACCATTGGCTGTATT
AAAGGATTTTAGAAAACATC

Downstream 100 bases:

>100_bases
TGGCAACTATTTTTAAAAGAACACCCGAACAATTGTCTGAAAGGCGGATTGATCCTTGGCACTATCAACCGCGATTTGAA
AGACAAATTAATAAGATAAA

Product: type I restriction-modification system methyltransferase subunit

Products: NA

Alternate protein names: M.MamI; Adenine-specific methyltransferase MamI [H]

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAGLIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDL
AYQLLSDIEGDWPVYAVVDDGLNQRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFDWQYVESHGDKAVAQIKAAFEEFKNH
ENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEAIQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESK
GGLGVYLTPNPVKQAMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADDKKESLKQK
LRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSFDLICTNPPFGTPKFDKGKNGQNSKANYEAN
MEQVLGGFRPTKKVVDSYNAWYDHVKMKWEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPD
SKGNWQPVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEFVGGKAIVKAVISLPSD
CFKLSGTGAKTSILYLQKRHANPKQPEQFLPEPQTDVFMAVAETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE

Sequences:

>Translated_715_residues
MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAGLIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDL
AYQLLSDIEGDWPVYAVVDDGLNQRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFDWQYVESHGDKAVAQIKAAFEEFKNH
ENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEAIQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESK
GGLGVYLTPNPVKQAMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADDKKESLKQK
LRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSFDLICTNPPFGTPKFDKGKNGQNSKANYEAN
MEQVLGGFRPTKKVVDSYNAWYDHVKMKWEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPD
SKGNWQPVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEFVGGKAIVKAVISLPSD
CFKLSGTGAKTSILYLQKRHANPKQPEQFLPEPQTDVFMAVAETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE
>Mature_714_residues
AKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAGLIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLA
YQLLSDIEGDWPVYAVVDDGLNQRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFHE
NVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFDWQYVESHGDKAVAQIKAAFEEFKNHE
NYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEAIQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKG
GLGVYLTPNPVKQAMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADDKKESLKQKL
RDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSFDLICTNPPFGTPKFDKGKNGQNSKANYEANM
EQVLGGFRPTKKVVDSYNAWYDHVKMKWEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDS
KGNWQPVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEFVGGKAIVKAVISLPSDC
FKLSGTGAKTSILYLQKRHANPKQPEQFLPEPQTDVFMAVAETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE

Specific function: This methylase recognizes the double-stranded sequence GATNNNNATC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the MamI endonuclease [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 80119; Mature: 79988

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAGLIQGLVEGNLTPPVML
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEE
IALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGLNQRILALFGADGSDGTHG
EEEECCCCHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCC
ADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFHENVYRVTKDKVNDKNDIIEE
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFDWQYVESHGDKAVAQIKAAFEEFKNH
HHHHHHHHHHHHCCCCCCEEECCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCC
ENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEAIQNLGPVKTNDGKIAKEHGTLADV
CCEEEECCCCCCCCCCCCCCCEEEHHHHCHHHHHHHHHHCCCCCCCCCCEECCCCCCHHC
SGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQAMLEIAMHDIDDDDEMRSRLANGDFR
CHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
FCDPTCGSFGFGSVALSQIDKWIDFKLVLADDKKESLKQKLRDCAFTGADAAPRMVMLAR
EECCCCCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VNMALQGAPKAQIFYTDNSLTTNALKPNSFDLICTNPPFGTPKFDKGKNGQNSKANYEAN
HHHHHCCCCCEEEEEECCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
MEQVLGGFRPTKKVVDSYNAWYDHVKMKWEDIGDLELDENGEPKWAGYRTDLRRTGGTDK
HHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEECCCCCCCCCCHHHHHHHCCCCCC
KPFYSLQPTTAGLALGSKPDSKGNWQPVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGI
CCEEEECCCHHCEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCE
LCNSGDRYVREYIMGKKDEKTGEFVGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRH
EECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHEECCCCCCEEEEEEEHHC
ANPKQPEQFLPEPQTDVFMAVAETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAGLIQGLVEGNLTPPVML
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEE
IALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGLNQRILALFGADGSDGTHG
EEEECCCCHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCC
ADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFHENVYRVTKDKVNDKNDIIEE
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFDWQYVESHGDKAVAQIKAAFEEFKNH
HHHHHHHHHHHHCCCCCCEEECCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCC
ENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEAIQNLGPVKTNDGKIAKEHGTLADV
CCEEEECCCCCCCCCCCCCCCEEEHHHHCHHHHHHHHHHCCCCCCCCCCEECCCCCCHHC
SGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQAMLEIAMHDIDDDDEMRSRLANGDFR
CHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
FCDPTCGSFGFGSVALSQIDKWIDFKLVLADDKKESLKQKLRDCAFTGADAAPRMVMLAR
EECCCCCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VNMALQGAPKAQIFYTDNSLTTNALKPNSFDLICTNPPFGTPKFDKGKNGQNSKANYEAN
HHHHHCCCCCEEEEEECCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
MEQVLGGFRPTKKVVDSYNAWYDHVKMKWEDIGDLELDENGEPKWAGYRTDLRRTGGTDK
HHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEECCCCCCCCCCHHHHHHHCCCCCC
KPFYSLQPTTAGLALGSKPDSKGNWQPVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGI
CCEEEECCCHHCEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCE
LCNSGDRYVREYIMGKKDEKTGEFVGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRH
EECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHEECCCCCCEEEEEEEHHC
ANPKQPEQFLPEPQTDVFMAVAETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8654988 [H]