Click here to switch to the map view.

The map label for this gene is yjiO [H]

Identifier: 218550398

GI number: 218550398

Start: 3176307

End: 3177539

Strand: Direct

Name: yjiO [H]

Synonym: EFER_3090

Alternate gene names: 218550398

Gene position: 3176307-3177539 (Clockwise)

Preceding gene: 218550396

Following gene: 218550399

Centisome position: 69.22

GC content: 54.26

Gene sequence:

>1233_bases
ATGCCACGTTTTTTTGCCCGCCATGCCGCCACGCTGTTTTTCCCGATGGCGTTAATTTTGTATGACTTTGCCGCGTACCT
GTCGACGGATCTAATCCAGCCAGGGATTATTAATGTGGTACGTGATTTTAATGCCGATGTCAGTCTGGCCCCTGCTGCCG
TCAGTCTCTATCTCGCTGGCGGTATGGCGTTACAGTGGCTATTGGGGCCACTTTCCGACAGAATTGGCCGCAGGCCAGTG
CTGATTACCGGGGCGCTTATTTTTACCCTTGCCTGCGCCGCGACAATGTTCACAACGTCTATGACACAGTTTCTTATCGC
GCGTGCAATTCAGGGCACCAGTATCTGTTTTATTGCCACCGTTGGTTATGTCACGGTGCAGGAGGCGTTCGGGCAGACAA
AAGGGATCAAGTTGATGGCGATTATCACCTCCATCGTACTGATTGCGCCAATTATCGGCCCGCTTTCCGGCGCAGCTCTT
ATGCACTTTATGCACTGGAAAGTCCTTTTTGCCATCATTGCGGTTATGGGTTTTATCTCATTTGTTGGCTTACTACTCGC
GATGCCAGAGACAGTAAAGCGCGGCGCGGTTCCGTTTAGCGCCAAAAGCGTACTGCGCGATTTTCGTAACGTTTTTTGCA
ACCGGCTGTTCCTCTTTGGCGCGGCAACCATCGCCCTCAGCTATATCCCAATGATGAGCTGGGTGGCTGTCTCGCCGGTG
ATCCTGATCGATGCGGGCGGCTTAACAACTACGCAGTTCGCCTGGACACAGGTACCGGTATTCGGCGCGGTGATTGTCGC
AAATGCCATCGTGGCGCGTTTTGTTAAAGATCCGACCGAACCGCGGTTTATCTGGCGTGCCGTACCTATTCAACTGGTCG
GCCTCGCGCTGTTGATTGTCGGCAATCTGCTGTCGCCGCATGTCTGGCTGTGGTCGGTGCTGGGCACCAGTCTGTATGCT
TTCGGGATTGGTTTGATTTTCCCGTCGCTATACCGCTTTACGCTCTTTTCGAACAACCTGCCGAAGGGCACCGTGTCGGC
ATCGCTGAATATCGTGGTTCTGACAGTGATGTCTGTCTCGGTCGAAATCGGCCGCTGGCTGTGGTTTAACGGCGGTCGCT
TGCCGTTTCATCTGTTAGCCGTTGTGGCGGGCGTTATCGTCGTTTTCACCCTGGCGGGATTGCTCAATCGCGTGCGCCAG
CATCAGGCAGCCGAGCTAGTGGAGGAGCAGTGA

Upstream 100 bases:

>100_bases
TTTGCAGTCTCGTTGCCCTTCTCACCTGCCTTTCGTCATTACCGACGGTATTGAATTTCGTTTTCCCCGTTGGGGTTCTC
CGGACAAGGAGTTGTTTGTT

Downstream 100 bases:

>100_bases
TTTTTGCGCGATCCTGCCGTCAGGCTCTATTCTTAACGTTATGAATAAACTCATCGAACTCAGACGCGCCAAAAGGTTGG
CGCTCTCTTTACTGCTTATC

Product: multidrug efflux system protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPV
LITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
MHFMHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATIALSYIPMMSWVAVSPV
ILIDAGGLTTTQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIVGNLLSPHVWLWSVLGTSLYA
FGIGLIFPSLYRFTLFSNNLPKGTVSASLNIVVLTVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQ
HQAAELVEEQ

Sequences:

>Translated_410_residues
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPV
LITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
MHFMHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATIALSYIPMMSWVAVSPV
ILIDAGGLTTTQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIVGNLLSPHVWLWSVLGTSLYA
FGIGLIFPSLYRFTLFSNNLPKGTVSASLNIVVLTVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQ
HQAAELVEEQ
>Mature_409_residues
PRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVL
ITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALM
HFMHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATIALSYIPMMSWVAVSPVI
LIDAGGLTTTQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIVGNLLSPHVWLWSVLGTSLYAF
GIGLIFPSLYRFTLFSNNLPKGTVSASLNIVVLTVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQH
QAAELVEEQ

Specific function: Confers resistance to acriflavine, chloramphenicol, norfloxacin, ethidium bromide and TPP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790794, Length=410, Percent_Identity=97.3170731707317, Blast_Score=797, Evalue=0.0,
Organism=Escherichia coli, GI1787065, Length=376, Percent_Identity=41.2234042553192, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1790146, Length=227, Percent_Identity=29.0748898678414, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994889, Length=221, Percent_Identity=27.1493212669683, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788509, Length=284, Percent_Identity=20.4225352112676, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082312, Length=184, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44536; Mature: 44405

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECHHHHHHHHHH
GMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIAT
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHH
VGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALMHFMHWKVLFAIIAVMGFIS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATIALSYIPMMSWVAVSPV
HHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
ILIDAGGLTTTQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
GNLLSPHVWLWSVLGTSLYAFGIGLIFPSLYRFTLFSNNLPKGTVSASLNIVVLTVMSVS
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHH
VEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQHQAAELVEEQ
HHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECHHHHHHHHHH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HHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]