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The map label for this gene is mcrB [H]

Identifier: 218550391

GI number: 218550391

Start: 3160286

End: 3161665

Strand: Direct

Name: mcrB [H]

Synonym: EFER_3083

Alternate gene names: 218550391

Gene position: 3160286-3161665 (Clockwise)

Preceding gene: 218550390

Following gene: 218550392

Centisome position: 68.87

GC content: 40.51

Gene sequence:

>1380_bases
ATGGAATCTATTCAACCCTGGATTGAAAAATTTATTAAGCAAGCACAGCAACAACGTTCGCAATCCACTAAAGATTATCC
AACGTCTTACCGTAACCTGCGAGTAAAATTGAGTTTCGGTTATGGTAATTTTACGTCTATTCCCTGGTTTGCATTTCTTG
GAGAAGGTCAGGAAGCTTCTAACGGTATATATCCCGTTATTCTCTATTATAAAGATTTTGATGAGTTGGTTTTGGCTTAT
GGTATAAGCGACACGAATGAACCACATGCCCAATGGCAGTTCTCTTCAGACATACCTAAAACAATCGCAGAGTATTTTCA
GGCAACTTCGGGTGTATATCCTAAAAAATACGGACAGTCCTATTACGCCTGTTCCCAAAAAGTCTCACAGGGTCTTGATT
ATACCCGGTTTGCCTCCATGCTGGACAACATAATCAACGACTATAAATTAATATTTAATTCTGGCAAGAGTGTTATTCCA
CCTATGTCAAAAACTGAATCATACTGTCTGGAAGATGCGTTAAATGATTTGTTTATCCCTGAAACCACAATAGAGACGAT
ACTCAAACGATTAACCATCAAAAAAAATATTATCCTCCAGGGGCCGCCCGGCGTTGGAAAAACCTTTGTTGCACGCCGTC
TGGCTTACTTGCTGACAGGAGAAAAGGCTCCGCAACGCGTCAATATGGTTCAGTTCCATCAATCTTATAGCTATGAGGAT
TTTATACAGGGCTATCGTCCGAATGGCGTCGGCTTCCGACGTAAAGACGGCATATTTTACAATTTTTGTCAGCAAGCTAA
AGAGCAGCCAGAGAAAAAGTATGTTTTTATTATAGATGAAATCAATCGTGCCAATCTCAGTAAAGTATTTGGCGAAGTGA
TGATGTTAATGGAACATGATAAACGAGGTGAAAACTGGTCTGTTCCCCTAACCTACTCCGAAAACGATGAAGAACGATTC
TATGTCCCGGAGAATGTTTATATCATCGGTTTAATGAATACTGCCGATCGCTCTCTGGCCGTTGTTGACTATGCCCTACG
CAGACGATTTTCTTTCATAGATATTGAGCCAGGTTTTGATACACCACAGTTCCGGAATTTTTTACTGAATAAAAAAGCAG
AACCTTCATTTGTTGAGTCTTTATGCCAAAAAATGAATGAGTTGAACCAGGAAATCAGCAAAGAGGCCACTATCCTTGGG
AAAGGATTCCGCATTGGGCATAGTTACTTCTGCTGTGGGTTGGAAGATGGCACATCTCCGGATACGCAATGGCTTAAGGA
AATTGTGATGACGGATATCGCCCCTTTACTCGAAGAATATTTCTTTGATGATCCCTATAAACAACAGATATGGGCCGACA
AATTATTAGGTGACTCATAG

Upstream 100 bases:

>100_bases
TCAGTAAATATTTACCAGTCTGATTTTGCAGTAAAAATCTATTGTTTCAGTACGTTGCGAAAGCGATAATAGAGGCTTAG
CAATGAGGAAGGCATATCTT

Downstream 100 bases:

>100_bases
TGGAACAGCCCGTGATACCTGTCCGTAATATCTATTACATGCTTACCTATGCATGGGGTTATTTACAGGAAATTAAGCAG
GCCGACCTTGAAGCCATTCC

Product: 5-methylcytosine-specific restriction enzyme subunit McrB

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MESIQPWIEKFIKQAQQQRSQSTKDYPTSYRNLRVKLSFGYGNFTSIPWFAFLGEGQEASNGIYPVILYYKDFDELVLAY
GISDTNEPHAQWQFSSDIPKTIAEYFQATSGVYPKKYGQSYYACSQKVSQGLDYTRFASMLDNIINDYKLIFNSGKSVIP
PMSKTESYCLEDALNDLFIPETTIETILKRLTIKKNIILQGPPGVGKTFVARRLAYLLTGEKAPQRVNMVQFHQSYSYED
FIQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYVFIIDEINRANLSKVFGEVMMLMEHDKRGENWSVPLTYSENDEERF
YVPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFDTPQFRNFLLNKKAEPSFVESLCQKMNELNQEISKEATILG
KGFRIGHSYFCCGLEDGTSPDTQWLKEIVMTDIAPLLEEYFFDDPYKQQIWADKLLGDS

Sequences:

>Translated_459_residues
MESIQPWIEKFIKQAQQQRSQSTKDYPTSYRNLRVKLSFGYGNFTSIPWFAFLGEGQEASNGIYPVILYYKDFDELVLAY
GISDTNEPHAQWQFSSDIPKTIAEYFQATSGVYPKKYGQSYYACSQKVSQGLDYTRFASMLDNIINDYKLIFNSGKSVIP
PMSKTESYCLEDALNDLFIPETTIETILKRLTIKKNIILQGPPGVGKTFVARRLAYLLTGEKAPQRVNMVQFHQSYSYED
FIQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYVFIIDEINRANLSKVFGEVMMLMEHDKRGENWSVPLTYSENDEERF
YVPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFDTPQFRNFLLNKKAEPSFVESLCQKMNELNQEISKEATILG
KGFRIGHSYFCCGLEDGTSPDTQWLKEIVMTDIAPLLEEYFFDDPYKQQIWADKLLGDS
>Mature_459_residues
MESIQPWIEKFIKQAQQQRSQSTKDYPTSYRNLRVKLSFGYGNFTSIPWFAFLGEGQEASNGIYPVILYYKDFDELVLAY
GISDTNEPHAQWQFSSDIPKTIAEYFQATSGVYPKKYGQSYYACSQKVSQGLDYTRFASMLDNIINDYKLIFNSGKSVIP
PMSKTESYCLEDALNDLFIPETTIETILKRLTIKKNIILQGPPGVGKTFVARRLAYLLTGEKAPQRVNMVQFHQSYSYED
FIQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYVFIIDEINRANLSKVFGEVMMLMEHDKRGENWSVPLTYSENDEERF
YVPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFDTPQFRNFLLNKKAEPSFVESLCQKMNELNQEISKEATILG
KGFRIGHSYFCCGLEDGTSPDTQWLKEIVMTDIAPLLEEYFFDDPYKQQIWADKLLGDS

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=459, Percent_Identity=98.6928104575163, Blast_Score=944, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 53114; Mature: 53114

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIQPWIEKFIKQAQQQRSQSTKDYPTSYRNLRVKLSFGYGNFTSIPWFAFLGEGQEAS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCC
NGIYPVILYYKDFDELVLAYGISDTNEPHAQWQFSSDIPKTIAEYFQATSGVYPKKYGQS
CCCEEEEEEECCHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHCCH
YYACSQKVSQGLDYTRFASMLDNIINDYKLIFNSGKSVIPPMSKTESYCLEDALNDLFIP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
ETTIETILKRLTIKKNIILQGPPGVGKTFVARRLAYLLTGEKAPQRVNMVQFHQSYSYED
HHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHH
FIQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYVFIIDEINRANLSKVFGEVMMLMEHD
HHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
KRGENWSVPLTYSENDEERFYVPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFD
CCCCCCCEEEEECCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCC
TPQFRNFLLNKKAEPSFVESLCQKMNELNQEISKEATILGKGFRIGHSYFCCGLEDGTSP
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEEECCCEEEEECCCCCCC
DTQWLKEIVMTDIAPLLEEYFFDDPYKQQIWADKLLGDS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MESIQPWIEKFIKQAQQQRSQSTKDYPTSYRNLRVKLSFGYGNFTSIPWFAFLGEGQEAS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCC
NGIYPVILYYKDFDELVLAYGISDTNEPHAQWQFSSDIPKTIAEYFQATSGVYPKKYGQS
CCCEEEEEEECCHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHCCH
YYACSQKVSQGLDYTRFASMLDNIINDYKLIFNSGKSVIPPMSKTESYCLEDALNDLFIP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
ETTIETILKRLTIKKNIILQGPPGVGKTFVARRLAYLLTGEKAPQRVNMVQFHQSYSYED
HHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHH
FIQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYVFIIDEINRANLSKVFGEVMMLMEHD
HHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
KRGENWSVPLTYSENDEERFYVPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFD
CCCCCCCEEEEECCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCC
TPQFRNFLLNKKAEPSFVESLCQKMNELNQEISKEATILGKGFRIGHSYFCCGLEDGTSP
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEEECCCEEEEECCCCCCC
DTQWLKEIVMTDIAPLLEEYFFDDPYKQQIWADKLLGDS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]