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The map label for this gene is mcrC [H]

Identifier: 218550392

GI number: 218550392

Start: 3161665

End: 3162711

Strand: Direct

Name: mcrC [H]

Synonym: EFER_3084

Alternate gene names: 218550392

Gene position: 3161665-3162711 (Clockwise)

Preceding gene: 218550391

Following gene: 218550396

Centisome position: 68.9

GC content: 38.3

Gene sequence:

>1047_bases
GTGGAACAGCCCGTGATACCTGTCCGTAATATCTATTACATGCTTACCTATGCATGGGGTTATTTACAGGAAATTAAGCA
GGCCGACCTTGAAGCCATTCCTGGTAACAATCTTCTTGATATCCTGGGGTATGTATTAAATAAAGGGGTTTTACAGCTTT
CACGCCGAGGGCTTGAGCTTGATTACAATCCTAATACCGAGATCATTCCTGGCATCAAAGGGCGAATAGAGTTTGCTAAA
ACAATACGCGGCTTCCATCTTAATCATGGGAAAACCGTCAGTACGTTTGATATGCTTAATGAAGACACGCTGGCTAACCG
AATTATAAAAAGCACATTAGCCATGTTAATTAAGCATGAAAAGTTAAATTCAGCCATCAGAGATGAAGCTCGTTCACTTT
ATCGGAAATTACCGGGCATTAGCACTCTTCATTTAACTCCACAGCATTTCAGCTATCTGAATGGCGGAAAAAATACGCGT
TATTATAAATTCGTTATCAGTGTCTGCAAGTTCATCGTCAATAATTCTATTCCAGGTCAAAACAAAGGACACTACCGTTT
CTATGATTTTGAAAGAAACGAAAAAGAGATGTCATTACTTTATCAAAAGTTTCTTTATGAATTTTGCCGCCGTGAATTAA
CGTCTGCAAACACAACCCGCTCTTATTTAAAATGGGATGCATCGAGCATATCGGATCAGTCACTTAATTTGTTACCTCGA
ATGGAAACTGACATCACCATTCGCTCATCAGAAAAAATACTTATCGTTGACGCCAAATACTATAAGAGCATTTTTTCACG
ACGAATGGGCTCAGAAAAATTTCATTCTCAAAATCTTTATCAACTGATGAATTACTTATGGTCGTTAAAGCCTGAAAATG
GCGAAAACATAGGGGGCTTATTAATATACCCCCACGTAGATACCGCAGTGAAACATCGTTATAAAATTAATGGCTTCGAT
ATTGGCTTGTGTACCGTCAATTTAGGTCAGGAATGGCCGTGTATACATCAAGAATTACTCGACATTTTCGATGAATATCT
CAAATAA

Upstream 100 bases:

>100_bases
AAATTGTGATGACGGATATCGCCCCTTTACTCGAAGAATATTTCTTTGATGATCCCTATAAACAACAGATATGGGCCGAC
AAATTATTAGGTGACTCATA

Downstream 100 bases:

>100_bases
GCGGTAAGTTTTATAGAAAATACCGCTCCCGACACCACATCGGGAGCGGCTCATCATCAGAAGGCGATCACCTCCACCAG
CACATCCGTTGCCCAACTCT

Product: 5-methylcytosine-specific restriction enzyme subunit McrC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MEQPVIPVRNIYYMLTYAWGYLQEIKQADLEAIPGNNLLDILGYVLNKGVLQLSRRGLELDYNPNTEIIPGIKGRIEFAK
TIRGFHLNHGKTVSTFDMLNEDTLANRIIKSTLAMLIKHEKLNSAIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTR
YYKFVISVCKFIVNNSIPGQNKGHYRFYDFERNEKEMSLLYQKFLYEFCRRELTSANTTRSYLKWDASSISDQSLNLLPR
METDITIRSSEKILIVDAKYYKSIFSRRMGSEKFHSQNLYQLMNYLWSLKPENGENIGGLLIYPHVDTAVKHRYKINGFD
IGLCTVNLGQEWPCIHQELLDIFDEYLK

Sequences:

>Translated_348_residues
MEQPVIPVRNIYYMLTYAWGYLQEIKQADLEAIPGNNLLDILGYVLNKGVLQLSRRGLELDYNPNTEIIPGIKGRIEFAK
TIRGFHLNHGKTVSTFDMLNEDTLANRIIKSTLAMLIKHEKLNSAIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTR
YYKFVISVCKFIVNNSIPGQNKGHYRFYDFERNEKEMSLLYQKFLYEFCRRELTSANTTRSYLKWDASSISDQSLNLLPR
METDITIRSSEKILIVDAKYYKSIFSRRMGSEKFHSQNLYQLMNYLWSLKPENGENIGGLLIYPHVDTAVKHRYKINGFD
IGLCTVNLGQEWPCIHQELLDIFDEYLK
>Mature_348_residues
MEQPVIPVRNIYYMLTYAWGYLQEIKQADLEAIPGNNLLDILGYVLNKGVLQLSRRGLELDYNPNTEIIPGIKGRIEFAK
TIRGFHLNHGKTVSTFDMLNEDTLANRIIKSTLAMLIKHEKLNSAIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTR
YYKFVISVCKFIVNNSIPGQNKGHYRFYDFERNEKEMSLLYQKFLYEFCRRELTSANTTRSYLKWDASSISDQSLNLLPR
METDITIRSSEKILIVDAKYYKSIFSRRMGSEKFHSQNLYQLMNYLWSLKPENGENIGGLLIYPHVDTAVKHRYKINGFD
IGLCTVNLGQEWPCIHQELLDIFDEYLK

Specific function: Modifies the specificity of mcrB restriction by expanding the range of modified sequences restricted. Does not bind to DNA [H]

COG id: COG4268

COG function: function code V; McrBC 5-methylcytosine restriction system component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790804, Length=348, Percent_Identity=98.8505747126437, Blast_Score=714, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014407
- InterPro:   IPR019292 [H]

Pfam domain/function: PF10117 McrBC [H]

EC number: NA

Molecular weight: Translated: 40565; Mature: 40565

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQPVIPVRNIYYMLTYAWGYLQEIKQADLEAIPGNNLLDILGYVLNKGVLQLSRRGLEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
DYNPNTEIIPGIKGRIEFAKTIRGFHLNHGKTVSTFDMLNEDTLANRIIKSTLAMLIKHE
CCCCCCCCCCCCCHHHHHHHHHHCEECCCCCEEHHHHHHCHHHHHHHHHHHHHHHHHHHH
KLNSAIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTRYYKFVISVCKFIVNNSIPGQ
HHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
NKGHYRFYDFERNEKEMSLLYQKFLYEFCRRELTSANTTRSYLKWDASSISDQSLNLLPR
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHCCCC
METDITIRSSEKILIVDAKYYKSIFSRRMGSEKFHSQNLYQLMNYLWSLKPENGENIGGL
CCCCEEEECCCEEEEEEHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCCE
LIYPHVDTAVKHRYKINGFDIGLCTVNLGQEWPCIHQELLDIFDEYLK
EEECCCCHHHHHEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEQPVIPVRNIYYMLTYAWGYLQEIKQADLEAIPGNNLLDILGYVLNKGVLQLSRRGLEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
DYNPNTEIIPGIKGRIEFAKTIRGFHLNHGKTVSTFDMLNEDTLANRIIKSTLAMLIKHE
CCCCCCCCCCCCCHHHHHHHHHHCEECCCCCEEHHHHHHCHHHHHHHHHHHHHHHHHHHH
KLNSAIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTRYYKFVISVCKFIVNNSIPGQ
HHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
NKGHYRFYDFERNEKEMSLLYQKFLYEFCRRELTSANTTRSYLKWDASSISDQSLNLLPR
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHCCCC
METDITIRSSEKILIVDAKYYKSIFSRRMGSEKFHSQNLYQLMNYLWSLKPENGENIGGL
CCCCEEEECCCEEEEEEHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCCE
LIYPHVDTAVKHRYKINGFDIGLCTVNLGQEWPCIHQELLDIFDEYLK
EEECCCCHHHHHEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 2050643 [H]