Click here to switch to the map view.

The map label for this gene is hpaH [H]

Identifier: 218550374

GI number: 218550374

Start: 3141106

End: 3141927

Strand: Direct

Name: hpaH [H]

Synonym: EFER_3066

Alternate gene names: 218550374

Gene position: 3141106-3141927 (Clockwise)

Preceding gene: 218550373

Following gene: 218550375

Centisome position: 68.45

GC content: 57.3

Gene sequence:

>822_bases
ATGCCACAGGATATCGCTATGTTCGATAAACACACCCACACCCTGATCGCCCAGCGTCTGGATCAGGCAGAAAAACAGCG
CGAACAGATCCGCGCGATCTCCCTCGATTACCCGGAGATCACCATCGAAGACGCCTACGCAGTACAGCGCGAATGGGTCC
GCCTGAAAATTGCCGAAGGCCGCACGCTGAAAGGCCACAAAATCGGCCTGACCTCGAAAGCGATGCAGGCCAGCTCGCAG
ATCAGCGAACCCGACTACGGTGCCCTGCTGGATGACATGTTCTTCCACGATGGCAGCGATATCCCGACCGATCGCTTTAT
CGTGCCGCGCATCGAAGTGGAGCTGGCTTTCGTGCTGGCAAAACCGCTGCGTGGGCCAAACTGCACGCTGTTCGACGTTT
ACAACGCCACGGACTATGTGATCCCGGCGTTGGAGTTGATCGACGCTCGCTGCCACAACATCGATCCAGAAACCCAGCGC
CCGCGCAAAGTGTTCGACACTATTTCCGATAACGCCGCCAATGCCGGGGTGATCCTCGGCGGTCGTCCTATCAAACCTGA
CGAACTGGATCTGCGTTGGATCTCCGCCCTGATGTATCGCAATGGCGTGATTGAAGAAACCGGCGTCGCTGCTGGTGTGC
TAAATCATCCGGCAAACGGCGTGGCCTGGCTGGCGAACAAACTCGCACCGTATGACGTACAACTGGAAGCCGGACAAATC
ATTCTCGGCGGCTCGTTCACCCGACCGGTTCCGGCGCGTAAAGGCGACACCTTTCACGTCGATTACGGCAACATGGGTTC
CATTAGCTGCCGCTTTGTTTAA

Upstream 100 bases:

>100_bases
CACCCAACGTTGAATTTCAAACAGAACAACGTACACGCGTTGTTTAAATAAATTTGCACCAGCTTCCAGGCCGGATGATA
AGGCGCAGCCGCATCCGGCA

Downstream 100 bases:

>100_bases
GGAGAGAACGATGGAAAACAGCTTTAAAGCGGCGCTGAAAGCAGGCCGACCACAGATCGGATTATGGCTGGGGCTGAGTA
GTAGCTACAGTGCAGAGTTA

Product: 2-oxo-hepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPQDIAMFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQ
ISEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQR
PRKVFDTISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQI
ILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV

Sequences:

>Translated_273_residues
MPQDIAMFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQ
ISEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQR
PRKVFDTISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQI
ILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV
>Mature_272_residues
PQDIAMFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQI
SEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRP
RKVFDTISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQII
LGGSFTRPVPARKGDTFHVDYGNMGSISCRFV

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=263, Percent_Identity=34.2205323193916, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.-

Molecular weight: Translated: 30371; Mature: 30240

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQDIAMFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEECHHHHHHHEEEEEEECCC
RTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLA
CEECCCEECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEECEEHEEEEEEEC
KPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFDTISDNAANAGVILG
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEC
GRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEECCEE
ILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV
EECCCCCCCCCCCCCCEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
PQDIAMFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEECHHHHHHHEEEEEEECCC
RTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLA
CEECCCEECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEECEEHEEEEEEEC
KPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFDTISDNAANAGVILG
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEC
GRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEECCEE
ILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV
EECCCCCCCCCCCCCCEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]