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The map label for this gene is hpaF [H]

Identifier: 218550373

GI number: 218550373

Start: 3140676

End: 3141056

Strand: Direct

Name: hpaF [H]

Synonym: EFER_3065

Alternate gene names: 218550373

Gene position: 3140676-3141056 (Clockwise)

Preceding gene: 218550372

Following gene: 218550374

Centisome position: 68.44

GC content: 51.18

Gene sequence:

>381_bases
ATGCCGCACTTTATCGTGGAATGTACCGAAAATATCCGTGAAGAAGCCCGCTTACCTGAGCTGTTTGCCAGTGTTAACAC
CGCACTGGCGGCGACGGGGATTTTTCCGCTTGGGGGAATACGTAGCCGGGCGCACTGGATCGATACCTGGCAAATGGCTG
ATGGCAAGCACGATTACGCCTTTGTGCATATGACGCTAAAAATCGGCAGCGGGCGCAGTCTGGAAAGCCGTCAGGAAGTG
GGCGAAATGCTGTTTGATCTGATTAAAACGCACTTCGCCTCACTGATGGAGAGCCGTTACCTGGCGCTTTCATTTGAGCT
TGCAGAGCTGCACCCAACGTTGAATTTCAAACAGAACAACGTACACGCGTTGTTTAAATAA

Upstream 100 bases:

>100_bases
TAAATACGATGGCAAGGTGGAGTTTATTACCGAGCTGTTCCCAAGCTCTGGCACCGGTCAGGTTAACGCTGTTTTCCCGC
TGCCCGCATAAGGAACCACC

Downstream 100 bases:

>100_bases
ATTTGCACCAGCTTCCAGGCCGGATGATAAGGCGCAGCCGCATCCGGCAATGCCACAGGATATCGCTATGTTCGATAAAC
ACACCCACACCCTGATCGCC

Product: 5-carboxymethyl-2-hydroxymuconate delta-isomerase

Products: NA

Alternate protein names: 5-carboxymethyl-2-hydroxymuconic acid isomerase; CHM isomerase; CHMI [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEV
GEMLFDLIKTHFASLMESRYLALSFELAELHPTLNFKQNNVHALFK

Sequences:

>Translated_126_residues
MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEV
GEMLFDLIKTHFASLMESRYLALSFELAELHPTLNFKQNNVHALFK
>Mature_125_residues
PHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVG
EMLFDLIKTHFASLMESRYLALSFELAELHPTLNFKQNNVHALFK

Specific function: Transforms 5-carboxymethyl-2-hydroxy-muconic acid (CHM) into 5-oxo-pent-3-ene-1,2,5-tricarboxylic acid (OPET) [H]

COG id: COG3232

COG function: function code E; 5-carboxymethyl-2-hydroxymuconate isomerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004220
- InterPro:   IPR014347 [H]

Pfam domain/function: PF02962 CHMI [H]

EC number: =5.3.3.10 [H]

Molecular weight: Translated: 14361; Mature: 14229

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEECCCCCCCEE
FVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLALSFELAELHPTLNFKQNN
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHHHCCCCCCCCCC
VHALFK
EEEECC
>Mature Secondary Structure 
PHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEECCCCCCCEE
FVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLALSFELAELHPTLNFKQNN
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHHHCCCCCCCCCC
VHALFK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194841; 2261999; 8547259 [H]