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The map label for this gene is mdtE [H]

Identifier: 218550346

GI number: 218550346

Start: 3109149

End: 3110438

Strand: Direct

Name: mdtE [H]

Synonym: EFER_3038

Alternate gene names: 218550346

Gene position: 3109149-3110438 (Clockwise)

Preceding gene: 218550345

Following gene: 218550347

Centisome position: 67.76

GC content: 50.7

Gene sequence:

>1290_bases
ATGTCGCCGGGTGGATGTTTATCTCTGGTTAATCATCCACCTGTCCAAACAGTAACGGTCATGCCCCTGTTCTGCAAACT
AAGTTCAAATAATCAGACTATTAAGCAAGCTATCCGTTATTCAAGGAACATGATGAACAAAAAGATAAAACTGCTGATAC
CGTTGTTGGTCTGCACCACGATGCTCACCGCTTGCGATAACAAGCCCGCTGAAAATACTTCTGCAGCCCTTCCAAAGGTG
GGCGTTGTCACTATAACTCCTGGCACTGTCAGCGTTACGAGTGAGCTTCCTGGCAGAACTGTCCCCTTTGAAATTGCCGA
GATACGACCACAGGTCGGCGGCATTATCGTAAAACGTAATTTTATTGAAGGTGACAAAGTTAAGCAGGGGGATTCACTCT
ATCAGATAGACCCGGCGCCTTTAAAAGCTGCCCTCGACTCAGCCAAAGGTAACCTGGCCAAGGCGCAGTCGGCCGCCAGT
AACGCGCGGTTAACCTTTAACCGTCAGTCCTCCCTTCTGCACTCAAATTATGTCAGCCGTCAGGATTACGACAATGCGCG
TAGCCAGCTTAATGAAGCCGAAGCCAATGTTACCGTGGCAAAAGCGGATGTCGAGCAAGCGGCAATCAACCTCAAATACG
CCAATGTGACGTCACCTATTACCGGGATAAGCGGGAAATCGTCAGTCACCGTCGGGGCACTGGTGACGGCAAATCAGAGC
GAAGCCCTGGTGACAGTGCAGCGGCTTGATCCGATTTATGTCGATCTAACTCAATCTGTACAAGAATTTCTGCGCCTGAA
AGAGGAAATCGCCAGCGGGCAAATACAGCAGGTAAAAGGACAAACACCAGTGGCGCTAAATCTGGAAAATGGCAAACGCT
ATGCCCAGACTGGCACCCTGAAGTTTTCTGATCCTACTGTCGATGAGACCACTGGCTCCGTAACGTTGCGGGCAATCTTC
CCTAACCCGAATGGCGATCTGTTACCCGGTATGTACGTCACTGCCCTGCTCGATGAAGGCAGTCGCCAGAATGTGCTAAT
GGTGCCGCAAGAAGGGGTAACGCATAACGCTCAGGGGAAAGCGACAGCATTAATTCTCGATAAAGACGATGTTGTCCAGC
AGCGCGAGATTAACGCGGTAAAAGCTATCGGCAATCAGTGGTTGGTCACGGCAGGATTACAACCTGGGGATCGGGTGATC
GTCTCTGGGCTACAACGGATCCGTCCAGGTATAAAAGCCCAGGCAATATCCTCCGACGAGAACACGGCCAGCAACCCGGC
TAAACAGTAA

Upstream 100 bases:

>100_bases
AGCCACTAAGGAAATAGTGACAAAACTGATTAACGGGCATAAATGGGAGGGAACCGAAAATATTTAAAGATGTTTCAGGC
TGGAGCAGCCAGCATTATTT

Downstream 100 bases:

>100_bases
CGTTGCAGGCATTAAGGGGATTTTCATGGCTAACTATTTTATTGAGCGCCCGGTGTTTGCATGGGTACTTGCAATTATTT
TGATGCTTGCAGGCGGGATC

Product: multidrug efflux system protein MdtE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSPGGCLSLVNHPPVQTVTVMPLFCKLSSNNQTIKQAIRYSRNMMNKKIKLLIPLLVCTTMLTACDNKPAENTSAALPKV
GVVTITPGTVSVTSELPGRTVPFEIAEIRPQVGGIIVKRNFIEGDKVKQGDSLYQIDPAPLKAALDSAKGNLAKAQSAAS
NARLTFNRQSSLLHSNYVSRQDYDNARSQLNEAEANVTVAKADVEQAAINLKYANVTSPITGISGKSSVTVGALVTANQS
EALVTVQRLDPIYVDLTQSVQEFLRLKEEIASGQIQQVKGQTPVALNLENGKRYAQTGTLKFSDPTVDETTGSVTLRAIF
PNPNGDLLPGMYVTALLDEGSRQNVLMVPQEGVTHNAQGKATALILDKDDVVQQREINAVKAIGNQWLVTAGLQPGDRVI
VSGLQRIRPGIKAQAISSDENTASNPAKQ

Sequences:

>Translated_429_residues
MSPGGCLSLVNHPPVQTVTVMPLFCKLSSNNQTIKQAIRYSRNMMNKKIKLLIPLLVCTTMLTACDNKPAENTSAALPKV
GVVTITPGTVSVTSELPGRTVPFEIAEIRPQVGGIIVKRNFIEGDKVKQGDSLYQIDPAPLKAALDSAKGNLAKAQSAAS
NARLTFNRQSSLLHSNYVSRQDYDNARSQLNEAEANVTVAKADVEQAAINLKYANVTSPITGISGKSSVTVGALVTANQS
EALVTVQRLDPIYVDLTQSVQEFLRLKEEIASGQIQQVKGQTPVALNLENGKRYAQTGTLKFSDPTVDETTGSVTLRAIF
PNPNGDLLPGMYVTALLDEGSRQNVLMVPQEGVTHNAQGKATALILDKDDVVQQREINAVKAIGNQWLVTAGLQPGDRVI
VSGLQRIRPGIKAQAISSDENTASNPAKQ
>Mature_428_residues
SPGGCLSLVNHPPVQTVTVMPLFCKLSSNNQTIKQAIRYSRNMMNKKIKLLIPLLVCTTMLTACDNKPAENTSAALPKVG
VVTITPGTVSVTSELPGRTVPFEIAEIRPQVGGIIVKRNFIEGDKVKQGDSLYQIDPAPLKAALDSAKGNLAKAQSAASN
ARLTFNRQSSLLHSNYVSRQDYDNARSQLNEAEANVTVAKADVEQAAINLKYANVTSPITGISGKSSVTVGALVTANQSE
ALVTVQRLDPIYVDLTQSVQEFLRLKEEIASGQIQQVKGQTPVALNLENGKRYAQTGTLKFSDPTVDETTGSVTLRAIFP
NPNGDLLPGMYVTALLDEGSRQNVLMVPQEGVTHNAQGKATALILDKDDVVQQREINAVKAIGNQWLVTAGLQPGDRVIV
SGLQRIRPGIKAQAISSDENTASNPAKQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=385, Percent_Identity=82.8571428571429, Blast_Score=661, Evalue=0.0,
Organism=Escherichia coli, GI1786668, Length=372, Percent_Identity=54.8387096774194, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1789665, Length=369, Percent_Identity=48.5094850948509, Blast_Score=341, Evalue=6e-95,
Organism=Escherichia coli, GI87082036, Length=318, Percent_Identity=28.3018867924528, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI87081796, Length=346, Percent_Identity=22.5433526011561, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081951, Length=129, Percent_Identity=32.5581395348837, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45946; Mature: 45815

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPGGCLSLVNHPPVQTVTVMPLFCKLSSNNQTIKQAIRYSRNMMNKKIKLLIPLLVCTT
CCCCCEEEECCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLTACDNKPAENTSAALPKVGVVTITPGTVSVTSELPGRTVPFEIAEIRPQVGGIIVKRN
HHHHCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCEEHHHCCCCCCCEEEEEC
FIEGDKVKQGDSLYQIDPAPLKAALDSAKGNLAKAQSAASNARLTFNRQSSLLHSNYVSR
CCCCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCCEEEECCHHHHHHHHCCCC
QDYDNARSQLNEAEANVTVAKADVEQAAINLKYANVTSPITGISGKSSVTVGALVTANQS
CCHHHHHHHHHHHHCCEEEEECCHHHHEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCC
EALVTVQRLDPIYVDLTQSVQEFLRLKEEIASGQIQQVKGQTPVALNLENGKRYAQTGTL
CEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCEEEEECEE
KFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALLDEGSRQNVLMVPQEGVTHNAQGK
EECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
ATALILDKDDVVQQREINAVKAIGNQWLVTAGLQPGDRVIVSGLQRIRPGIKAQAISSDE
EEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCC
NTASNPAKQ
CCCCCCCCC
>Mature Secondary Structure 
SPGGCLSLVNHPPVQTVTVMPLFCKLSSNNQTIKQAIRYSRNMMNKKIKLLIPLLVCTT
CCCCEEEECCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLTACDNKPAENTSAALPKVGVVTITPGTVSVTSELPGRTVPFEIAEIRPQVGGIIVKRN
HHHHCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCEEHHHCCCCCCCEEEEEC
FIEGDKVKQGDSLYQIDPAPLKAALDSAKGNLAKAQSAASNARLTFNRQSSLLHSNYVSR
CCCCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCCEEEECCHHHHHHHHCCCC
QDYDNARSQLNEAEANVTVAKADVEQAAINLKYANVTSPITGISGKSSVTVGALVTANQS
CCHHHHHHHHHHHHCCEEEEECCHHHHEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCC
EALVTVQRLDPIYVDLTQSVQEFLRLKEEIASGQIQQVKGQTPVALNLENGKRYAQTGTL
CEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCEEEEECEE
KFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALLDEGSRQNVLMVPQEGVTHNAQGK
EECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
ATALILDKDDVVQQREINAVKAIGNQWLVTAGLQPGDRVIVSGLQRIRPGIKAQAISSDE
EEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCC
NTASNPAKQ
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]