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The map label for this gene is gadE [H]

Identifier: 218550345

GI number: 218550345

Start: 3108502

End: 3109029

Strand: Direct

Name: gadE [H]

Synonym: EFER_3037

Alternate gene names: 218550345

Gene position: 3108502-3109029 (Clockwise)

Preceding gene: 218550344

Following gene: 218550346

Centisome position: 67.74

GC content: 33.52

Gene sequence:

>528_bases
ATGATTTTTCTCATGACGAAAGATTCTTTTCTGTTACACGGATTAATGAATTTAAAAGATGATCGTAAAGTGATAAAAAT
CAATAATCTTTATGATATCAATAATATATCTGACATGCCATTCAAAGTCGTTATTGATACCTATCATAACCACATCCTTG
ACGAAGAATCGATGGAGTTTCTCGCCAGTCTCAATGCGGAAAGAATCATCATCTTAGCGCCCTACCATATCAGCAAACTA
AAAAGTAACGCCCCGATTTACTTTATCAACCGCAACGAAAGTCTTAAGAATCTTACTGAAATTATTTATGGCAAACCATT
ACACCATAAAAACGACCAACTTTGCTTTTCTCACAATCAGTTTAAGATTATGCAACTGATTCTCAAAAACAAAAACGAAA
GCAATATTACTTCAGCGCTCAACATCTCTCAACAGACGCTAAAGATTCAGAAATTCAATATTATGTACAAGTTAAAGCTT
AGAAGAATGAGCGATATTGTTACGCTGGGTATTTCATCCTATTTTTAG

Upstream 100 bases:

>100_bases
ATGTCATCAATGCTTGCTATAGAGAACAATGTTTAAAATCAGGATGTTATAAATCTTAATGGACCACATGTTCGCAGTCA
AAATAGCTAAGGAGTAAGTT

Downstream 100 bases:

>100_bases
CCACATAATTATCAGAATTAGCCACTAAGGAAATAGTGACAAAACTGATTAACGGGCATAAATGGGAGGGAACCGAAAAT
ATTTAAAGATGTTTCAGGCT

Product: transcriptional regulator gadE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MIFLMTKDSFLLHGLMNLKDDRKVIKINNLYDINNISDMPFKVVIDTYHNHILDEESMEFLASLNAERIIILAPYHISKL
KSNAPIYFINRNESLKNLTEIIYGKPLHHKNDQLCFSHNQFKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGISSYF

Sequences:

>Translated_175_residues
MIFLMTKDSFLLHGLMNLKDDRKVIKINNLYDINNISDMPFKVVIDTYHNHILDEESMEFLASLNAERIIILAPYHISKL
KSNAPIYFINRNESLKNLTEIIYGKPLHHKNDQLCFSHNQFKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGISSYF
>Mature_175_residues
MIFLMTKDSFLLHGLMNLKDDRKVIKINNLYDINNISDMPFKVVIDTYHNHILDEESMEFLASLNAERIIILAPYHISKL
KSNAPIYFINRNESLKNLTEIIYGKPLHHKNDQLCFSHNQFKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGISSYF

Specific function: Regulates the expression of several genes involved in acid resistance. Required for the expression of gadA and gadBC, among others, regardless of media or growth conditions. Binds directly to the 20 bp GAD box found in the control regions of both loci. Co

COG id: COG2771

COG function: function code K; DNA-binding HTH domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789928, Length=175, Percent_Identity=78.2857142857143, Blast_Score=281, Evalue=1e-77,
Organism=Escherichia coli, GI1789923, Length=167, Percent_Identity=23.3532934131737, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991
- InterPro:   IPR013022 [H]

Pfam domain/function: PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 20549; Mature: 20549

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLMTKDSFLLHGLMNLKDDRKVIKINNLYDINNISDMPFKVVIDTYHNHILDEESMEF
CEEEEECCHHHHHHHHCCCCCCEEEEEECEEECCCCCCCCEEEEEECHHHHCCCHHHHHH
LASLNAERIIILAPYHISKLKSNAPIYFINRNESLKNLTEIIYGKPLHHKNDQLCFSHNQ
HHHCCCCEEEEECCCHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEECCH
FKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKLRRMSDIVTLGISSYF
HHHHHHHHHCCCCCCHHHHHCCCHHHEEEEEEEEEEEEEHHHHHHHEEEEHHCCC
>Mature Secondary Structure
MIFLMTKDSFLLHGLMNLKDDRKVIKINNLYDINNISDMPFKVVIDTYHNHILDEESMEF
CEEEEECCHHHHHHHHCCCCCCEEEEEECEEECCCCCCCCEEEEEECHHHHCCCHHHHHH
LASLNAERIIILAPYHISKLKSNAPIYFINRNESLKNLTEIIYGKPLHHKNDQLCFSHNQ
HHHCCCCEEEEECCCHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEECCH
FKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKLRRMSDIVTLGISSYF
HHHHHHHHHCCCCCCHHHHHCCCHHHEEEEEEEEEEEEEHHHHHHHEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]