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The map label for this gene is ubiH [H]

Identifier: 218550155

GI number: 218550155

Start: 2911115

End: 2912293

Strand: Reverse

Name: ubiH [H]

Synonym: EFER_2844

Alternate gene names: 218550155

Gene position: 2912293-2911115 (Counterclockwise)

Preceding gene: 218550156

Following gene: 218550154

Centisome position: 63.47

GC content: 59.97

Gene sequence:

>1179_bases
ATGAGCGTAATCATCGTCGGTGGCGGCATGGCGGGCGCGACGCTGGCGCTGGCTATTTCCCAGTTAAGTCACGGGGCGCT
GCCGGTACATTTGATTGAAGCGACCGCGCCAGAGTCTCATGCTCATCCGGGCTTTGATGGACGGGCTATTGCGCTGGCGG
CGGGCACCTGTCAGCAACTGGTGCGCATCGGCGTCTGGCAATCTCTGGCAGATTGCGCAACCGCCATCACTACCGTTCAT
GTCAGCGATCGTGGTCATGCCGGATTCGTCACCCTCGCCGCAGAAGATTATCAACTGGCGGCGCTGGGGCAGGTTGTCGA
ATTGCATAATGTCGGGCAACGGCTGTTTGCGCTATTGCGCAAAGCGCCTGGCGTAACGCTGCATTGTCCGGATCGCGTGG
CTAACGTTGCCCGTACTCAGAGTCACGTTGAAGTGACACTGGAGAGTGGCAAGACGCTGACGGGCCGTGTGCTGGTCGCT
GCTGATGGCACCCATTCCGCGTTAGCCACCGCGTGCGGTGTTGACTGGCAGCAGGAGCCTTACGAACAACTGGCCGTGAT
TGCGAACGTTGCGACTTCCGTTGCGCACGAAGGCCGGGCGTTTGAGCGTTTCACACAATATGGCCCGTTGGCGATGTTGC
CGATGTCTGACGGACGCTGTTCGCTGGTCTGGTGCCATCCACTGGAACGGCGCGAAGAGGTGCTGTCGTGGAGCGACGAG
ACGTTTTGCCGTGAACTCCAGTCGGCCTTTGGTTGGCGACTTGGGAAAATTACCCACGCTGGTAAACGCAGTGCTTACCC
GCTGGCGTTAACCCGCGCCGCCAGACCTGTTACCCATCGTACCGTGCTGGTGGGCAATGCGGCGCAAACTCTGCACCCGA
TTGCCGGGCAAGGGTTCAACCTCGGTATGCGAGATGTGATGAGCCTTGCGGAAACCCTGACTCAGGCGCAAGAGCGCGGA
GAAGACATAGGTGATTACGGCGTCTTGTGCCGTTATCAGCAGCGTCGACAGAGCGATCGAGAAGCAACCATCGGCGTCAC
GGACAGCCTTGTACATCTTTTTGCCAACCGTTGGACGCCGCTGGTTGTCGGGCGCAACATCGGGCTGATGGCAATGGAAT
TATTCACTCCGGCACGCGATGTGCTGGCGCAGCGCACCCTCGGTTGGGTGGCGCGTTGA

Upstream 100 bases:

>100_bases
ACGACATTGTGATTACCGAAACCGGCAATGAAAACCTCACCGCCAGCGTGGTGAAAAAGCCGGAAGAAATTGAAGCGTTG
ATGGCAGCTGCGAGAAAGCA

Downstream 100 bases:

>100_bases
AGGCGGTTTTAAGGAATAAACAATGCAAAGTGTTGATGTAGCCATTGTTGGCGGCGGCATGGTGGGGCTGGCGGTTGCCT
GCGGCTTACAGGGGAGCGGC

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSVIIVGGGMAGATLALAISQLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLVRIGVWQSLADCATAITTVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGKTLTGRVLVA
ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
TFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARPVTHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERG
EDIGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR

Sequences:

>Translated_392_residues
MSVIIVGGGMAGATLALAISQLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLVRIGVWQSLADCATAITTVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGKTLTGRVLVA
ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
TFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARPVTHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERG
EDIGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
>Mature_391_residues
SVIIVGGGMAGATLALAISQLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLVRIGVWQSLADCATAITTVHV
SDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGKTLTGRVLVAA
DGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDET
FCRELQSAFGWRLGKITHAGKRSAYPLALTRAARPVTHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGE
DIGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=29.2746113989637, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI32967307, Length=374, Percent_Identity=30.2139037433155, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=97.1938775510204, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=30.5343511450382, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786883, Length=373, Percent_Identity=32.4396782841823, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25146631, Length=425, Percent_Identity=26.1176470588235, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=25.3863134657837, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19920760, Length=420, Percent_Identity=23.8095238095238, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42284; Mature: 42153

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIIVGGGMAGATLALAISQLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
VRIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARTQSHVEVTLESGKTLTGRVLVAADGTHSALATACGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCH
TFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARPVTHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHCEECCCCCCCCCCHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAQERGEDIGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCE
LVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
EEEECCCCHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIIVGGGMAGATLALAISQLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
EEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
VRIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARTQSHVEVTLESGKTLTGRVLVAADGTHSALATACGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCH
TFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARPVTHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHCEECCCCCCCCCCHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAQERGEDIGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCE
LVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
EEEECCCCHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]