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The map label for this gene is insO2 [H]

Identifier: 218550113

GI number: 218550113

Start: 2865689

End: 2866528

Strand: Reverse

Name: insO2 [H]

Synonym: EFER_2799

Alternate gene names: 218550113

Gene position: 2866528-2865689 (Counterclockwise)

Preceding gene: 218550115

Following gene: 218550112

Centisome position: 62.47

GC content: 52.86

Gene sequence:

>840_bases
GTGGTCACACTCTGCCAAGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGGTGGAAAGCCCCGAAAAGCCAGACGG
CAAACGGGCTGTATTACGTAGTCAGGTTCTGGAGCTGCATAACATTAGCCATGGCTCTGCTGGCGCGAGAAGTATCGCCA
TTATGGCAACCCTGAGAGGCTTCAGAATGGGACGCTGGCTTGCCGGCAGGCTCATGAAAGAACTGGGACTGGTGAGTTGT
CAGCAACCCACCCACCGGTATAAACGTGGTGGTCATGAACACGTCGCTATCCCGAATCGCCTTGAGCGACAGTTCGCAGT
GACAGAGCCTAATCAGGTGTGGTGCGGCGATGTGACGTATATCTGGACAGGCAAGCGCTGGGCTTACCTTGCTGTTGTTC
TCGATCTGTTCGCAAGGAAACCGGTGGGCTGGGCAATGTCATTCTCACCGGACAGCAGGCTGACCATCAAAGCGCTGGAA
ATGGCATGGGAAGCTCGCGGTAAACCAGCCGGATTGATGTTCCACAGCGACCAGGGTAGCCACTATACAAGCAGGCAGTT
CCGGCAGTTACTGTGGCGTTGCCGGATCAGTCAGAGTATGAGCCGACGTGGAAACTGTTGGGATAACAGCCCGATGGAAC
GCTTCTTCAGAAGTCTTAAAAACGAGTGGGTGCCAGTAACAGGTTATGTAAGCTTTAGCGAAGCTGCTCATGCGATCACA
GACTATATCGTTGGGTATTACAGCTCGCTAAGACCGCATGACTATAACGGTGGGTTATCCCCAAACGAATCGGAAAACCG
ATACTGGAAAAACTCTAAAGCGGTGGCCAGTTTTAGTTGA

Upstream 100 bases:

>100_bases
TTGAAATGGAAAACGACATATTAAAAAAGGCTACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGTTAATCGGGA
AACTCAGAGCGCAGTATCCT

Downstream 100 bases:

>100_bases
CCACTACAGTGAAAGTATTCAGAGCGAACTGGTTATCAACTCCATCACTCCGGTACGTCTGACCTTCGGCGAGTTTCCAG
GCTACCGCAACGTCGTGCTG

Product: transposase ORF B, IS911

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MVTLCQVFGVHRSSYKYWVESPEKPDGKRAVLRSQVLELHNISHGSAGARSIAIMATLRGFRMGRWLAGRLMKELGLVSC
QQPTHRYKRGGHEHVAIPNRLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALE
MAWEARGKPAGLMFHSDQGSHYTSRQFRQLLWRCRISQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSEAAHAIT
DYIVGYYSSLRPHDYNGGLSPNESENRYWKNSKAVASFS

Sequences:

>Translated_279_residues
MVTLCQVFGVHRSSYKYWVESPEKPDGKRAVLRSQVLELHNISHGSAGARSIAIMATLRGFRMGRWLAGRLMKELGLVSC
QQPTHRYKRGGHEHVAIPNRLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALE
MAWEARGKPAGLMFHSDQGSHYTSRQFRQLLWRCRISQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSEAAHAIT
DYIVGYYSSLRPHDYNGGLSPNESENRYWKNSKAVASFS
>Mature_279_residues
MVTLCQVFGVHRSSYKYWVESPEKPDGKRAVLRSQVLELHNISHGSAGARSIAIMATLRGFRMGRWLAGRLMKELGLVSC
QQPTHRYKRGGHEHVAIPNRLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALE
MAWEARGKPAGLMFHSDQGSHYTSRQFRQLLWRCRISQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSEAAHAIT
DYIVGYYSSLRPHDYNGGLSPNESENRYWKNSKAVASFS

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=217, Percent_Identity=28.110599078341, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1789981, Length=267, Percent_Identity=32.2097378277154, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788405, Length=271, Percent_Identity=30.9963099630996, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1787263, Length=271, Percent_Identity=30.9963099630996, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1786753, Length=271, Percent_Identity=30.9963099630996, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1786570, Length=271, Percent_Identity=30.9963099630996, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1786490, Length=271, Percent_Identity=30.9963099630996, Blast_Score=130, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32045; Mature: 32045

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLCQVFGVHRSSYKYWVESPEKPDGKRAVLRSQVLELHNISHGSAGARSIAIMATLRG
CEEHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FRMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNRLERQFAVTEPNQVWCGDVTY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEECCCHHHHHCEECCCCCEEEECCEEE
IWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALEMAWEARGKPAGLMFHSDQGS
EEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCEEEEECCCCC
HYTSRQFRQLLWRCRISQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSEAAHAIT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEEEEEHHHHHHHHH
DYIVGYYSSLRPHDYNGGLSPNESENRYWKNSKAVASFS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure
MVTLCQVFGVHRSSYKYWVESPEKPDGKRAVLRSQVLELHNISHGSAGARSIAIMATLRG
CEEHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FRMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNRLERQFAVTEPNQVWCGDVTY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEECCCHHHHHCEECCCCCEEEECCEEE
IWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALEMAWEARGKPAGLMFHSDQGS
EEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCEEEEECCCCC
HYTSRQFRQLLWRCRISQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSEAAHAIT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEEEEEHHHHHHHHH
DYIVGYYSSLRPHDYNGGLSPNESENRYWKNSKAVASFS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]