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The map label for this gene is yafN

Identifier: 218550063

GI number: 218550063

Start: 2806752

End: 2807045

Strand: Reverse

Name: yafN

Synonym: EFER_2748

Alternate gene names: 218550063

Gene position: 2807045-2806752 (Counterclockwise)

Preceding gene: 218550064

Following gene: 218550062

Centisome position: 61.17

GC content: 41.16

Gene sequence:

>294_bases
ATGCATCGAATTCTTGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATATTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGTTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Upstream 100 bases:

>100_bases
GACGTTGCTGGATCCGCAAATGGAAAGACAACTGGTGTTGGGATTATGATGTATACTATTATGTATATTCTGGCGTGCAT
TATTATGAGGGTATCACTGT

Downstream 100 bases:

>100_bases
GGATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCCAAGGAACTTGATGCGTTAACGGCGGATTTTATT
TCCTATAAGCGTGACGGTGT

Product: antitoxin of the YafO-YafN toxin-antitoxin system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 97; Mature: 97

Protein sequence:

>97_residues
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Sequences:

>Translated_97_residues
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
>Mature_97_residues
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Specific function: Part of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin; overexpression prevents YafO-mediated cessation of cell growth and inhibition of cell proliferation

COG id: COG2161

COG function: function code D; Antitoxin of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786426, Length=97, Percent_Identity=100, Blast_Score=197, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YAFN_ECOLI (Q47156)

Other databases:

- EMBL:   D38582
- EMBL:   U70214
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64748
- RefSeq:   AP_000885.1
- RefSeq:   NP_414767.1
- ProteinModelPortal:   Q47156
- IntAct:   Q47156
- STRING:   Q47156
- EnsemblBacteria:   EBESCT00000004966
- EnsemblBacteria:   EBESCT00000015147
- GeneID:   944920
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0222
- KEGG:   eco:b0232
- EchoBASE:   EB2945
- EcoGene:   EG13151
- eggNOG:   COG2161
- GeneTree:   EBGT00050000011973
- HOGENOM:   HBG469431
- OMA:   LEACNPK
- ProtClustDB:   PRK09778
- BioCyc:   EcoCyc:G6116-MONOMER
- Genevestigator:   Q47156
- InterPro:   IPR006442
- TIGRFAMs:   TIGR01552

Pfam domain/function: PF02604 PhdYeFM

EC number: NA

Molecular weight: Translated: 11234; Mature: 11234

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKK
CCCCCCCCCCCHHHHHCCHHHHHHCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCC
PIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE
CHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKK
CCCCCCCCCCCHHHHHCCHHHHHHCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCC
PIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE
CHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503