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The map label for this gene is yafO [H]

Identifier: 218550062

GI number: 218550062

Start: 2806351

End: 2806749

Strand: Reverse

Name: yafO [H]

Synonym: EFER_2747

Alternate gene names: 218550062

Gene position: 2806749-2806351 (Counterclockwise)

Preceding gene: 218550063

Following gene: 218550061

Centisome position: 61.17

GC content: 43.36

Gene sequence:

>399_bases
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCCAAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACA
AATGACGAAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Upstream 100 bases:

>100_bases
TCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCTGAACAATATCTTAATGATATGACGGATGATGATTTC
AATGACTTTAAGGAATAAGG

Downstream 100 bases:

>100_bases
ATTTATATTATGAATAATATACAAATAAGAGACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTTATTAGAGC
GGTTACGATGACCGCCAGTC

Product: toxin YafO

Products: NA

Alternate protein names: Endoribonuclease YafO; Toxin YafO [H]

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Sequences:

>Translated_132_residues
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
>Mature_132_residues
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Specific function: Part of a toxin-antitoxin (TA) module. A translation- dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786427, Length=132, Percent_Identity=99.2424242424242, Blast_Score=274, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020353
- ProDom:   PD082093 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 15487; Mature: 15487

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAH
CCCHHCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
IHLANENNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHK
EEECCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEECCCCHHHHCCCCHHHH
IGKMAEAFRMRF
HHHHHHHHHCCC
>Mature Secondary Structure
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAH
CCCHHCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
IHLANENNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHK
EEECCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEECCCCHHHHCCCCHHHH
IGKMAEAFRMRF
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]