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The map label for this gene is yahA [H]

Identifier: 218549985

GI number: 218549985

Start: 2747733

End: 2748839

Strand: Reverse

Name: yahA [H]

Synonym: EFER_2670

Alternate gene names: 218549985

Gene position: 2748839-2747733 (Counterclockwise)

Preceding gene: 218549989

Following gene: 218549984

Centisome position: 59.9

GC content: 42.1

Gene sequence:

>1107_bases
ATGATGGTGTGTTCTATGGGGTTGTGTAATTTAAGTGTGTTTCTGGAAGAATTCGATGCGACAATAAATATCAATTTGCC
GCAGGTTCTGTCTGAGAAAGAACAACTGCTGCTTAAATTGTTAATGCAAGGTATGTCTGTTACAGAAATCTCACAATACA
GAAACCGTAGTGCAAAGACAATATCTCATCAAAAAAAACAGTTGTATGAAAAGTTGGGTATCCAGAGCGATATTACCTTC
TGGCGGGATATCTTCTTTTTGTATAACCCGATGATCATCTCTGCTTCTGGTAACAGTAATAAAAGCTACATAACCGATAA
TGGCTATCATCATGTTATTACGCCCGAAGCAATCAGCCTGGCGCTGGAAAATCATGAGTTTAAACCCTGGATTCAACCTG
TTTTCTGTGCTCAAACCGGTGTTCTGACAGGGTGTGAGATACTCGTCCGCTGGGAGCATCCACAAACAGGAATTATTCCA
CCAGACCAATTTATTCCTCTGGCGGAATCATCTGGTCTGATTGTTATATTAACCCGACAGTTAATGAAACAAACGGCAGA
TATATTACTGCCAGTAAAAGATTTGCTGCCTGATAATTTCCATATTGGCATAAATGTTTCTGCTGGTTGTTTTCTGGCAC
CCGGTTTTGAGCGCGAGTGTCTGAATCTCGTGAATAAACTGGGAACGGATAAAATCAAACTGGTTCTGGAATTGACGGAA
CGTAACCCAATCCCGGTGACGCCAGAGGCCAGAGCAATATTTGATAGCCTGCATCGGCATAACATTACCTTTGCTCTGGA
CGATTTTGGTACTGGCTATGCAACCTATCGTTATTTGCAGGCTTTTCCAGTTGATTTTATTAAGATTGATAAATCATTTG
TCCAGATGGCGAGTATCGATGAAATCTCCGGGCATATTGTGGACAATATCGTTGAGCTTGCCCGTAAGCCAGGCCTTAGT
ATTGTGGCGGAAGGCGTCGAAACTCAGGAACAGGCTGATTTGATGATTGGCAAAGGAGTACACTTTTTACAGGGGTATCT
CTACTCACCTCCATTGCCTGGTGCGAAATTTATCTCTGACTGGGTTAAAAACAGTGCTGTAGCATAA

Upstream 100 bases:

>100_bases
GAGTTGGATTGTGTAATAATCCGAGTCTTTTGTTAATTATTCTTGCACTCAGCTTATCCGCCGCGAGAGTGATAATTCGG
AGTCTGCTGATGCACTTTTT

Downstream 100 bases:

>100_bases
TGATATTGTATCTTTATCCACAGCACTCTCTGTTATACGTGCAGCATCCAATTAGACCGTTGCTGCACGTGTTTTGTTGT
TCTGCAACAAACACCATGTG

Product: cyclic di-GMP phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MMVCSMGLCNLSVFLEEFDATININLPQVLSEKEQLLLKLLMQGMSVTEISQYRNRSAKTISHQKKQLYEKLGIQSDITF
WRDIFFLYNPMIISASGNSNKSYITDNGYHHVITPEAISLALENHEFKPWIQPVFCAQTGVLTGCEILVRWEHPQTGIIP
PDQFIPLAESSGLIVILTRQLMKQTADILLPVKDLLPDNFHIGINVSAGCFLAPGFERECLNLVNKLGTDKIKLVLELTE
RNPIPVTPEARAIFDSLHRHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASIDEISGHIVDNIVELARKPGLS
IVAEGVETQEQADLMIGKGVHFLQGYLYSPPLPGAKFISDWVKNSAVA

Sequences:

>Translated_368_residues
MMVCSMGLCNLSVFLEEFDATININLPQVLSEKEQLLLKLLMQGMSVTEISQYRNRSAKTISHQKKQLYEKLGIQSDITF
WRDIFFLYNPMIISASGNSNKSYITDNGYHHVITPEAISLALENHEFKPWIQPVFCAQTGVLTGCEILVRWEHPQTGIIP
PDQFIPLAESSGLIVILTRQLMKQTADILLPVKDLLPDNFHIGINVSAGCFLAPGFERECLNLVNKLGTDKIKLVLELTE
RNPIPVTPEARAIFDSLHRHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASIDEISGHIVDNIVELARKPGLS
IVAEGVETQEQADLMIGKGVHFLQGYLYSPPLPGAKFISDWVKNSAVA
>Mature_368_residues
MMVCSMGLCNLSVFLEEFDATININLPQVLSEKEQLLLKLLMQGMSVTEISQYRNRSAKTISHQKKQLYEKLGIQSDITF
WRDIFFLYNPMIISASGNSNKSYITDNGYHHVITPEAISLALENHEFKPWIQPVFCAQTGVLTGCEILVRWEHPQTGIIP
PDQFIPLAESSGLIVILTRQLMKQTADILLPVKDLLPDNFHIGINVSAGCFLAPGFERECLNLVNKLGTDKIKLVLELTE
RNPIPVTPEARAIFDSLHRHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASIDEISGHIVDNIVELARKPGLS
IVAEGVETQEQADLMIGKGVHFLQGYLYSPPLPGAKFISDWVKNSAVA

Specific function: Hydrolyzes c-di-GMP (cyclic bis(3'-5') dimeric GMP) to 5'-pGpG, known as PDE-A activity. PDE-B activity, that is hydrolysis of 5'-pGpG to GMP, proceeds only very slowly [H]

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786507, Length=361, Percent_Identity=87.5346260387812, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI87081845, Length=252, Percent_Identity=44.047619047619, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788502, Length=247, Percent_Identity=37.246963562753, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI87081980, Length=250, Percent_Identity=40, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87081743, Length=241, Percent_Identity=34.4398340248963, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI226510982, Length=239, Percent_Identity=34.3096234309623, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081921, Length=251, Percent_Identity=32.6693227091633, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790496, Length=246, Percent_Identity=30.8943089430894, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787541, Length=243, Percent_Identity=31.6872427983539, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788849, Length=233, Percent_Identity=30.4721030042918, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787055, Length=252, Percent_Identity=30.5555555555556, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082096, Length=260, Percent_Identity=29.2307692307692, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633
- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00563 EAL; PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 41225; Mature: 41225

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50043 HTH_LUXR_2 ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVCSMGLCNLSVFLEEFDATININLPQVLSEKEQLLLKLLMQGMSVTEISQYRNRSAKT
CEEECCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHH
ISHQKKQLYEKLGIQSDITFWRDIFFLYNPMIISASGNSNKSYITDNGYHHVITPEAISL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCCEEECCCEEEEECHHHHEE
ALENHEFKPWIQPVFCAQTGVLTGCEILVRWEHPQTGIIPPDQFIPLAESSGLIVILTRQ
ECCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHH
LMKQTADILLPVKDLLPDNFHIGINVSAGCFLAPGFERECLNLVNKLGTDKIKLVLELTE
HHHHHHHHEEEHHHHCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHCCCEEEEEEEECC
RNPIPVTPEARAIFDSLHRHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASID
CCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKGVHFLQGYLYSPPLPGAKFISD
HHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
WVKNSAVA
HHHHCCCC
>Mature Secondary Structure
MMVCSMGLCNLSVFLEEFDATININLPQVLSEKEQLLLKLLMQGMSVTEISQYRNRSAKT
CEEECCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHH
ISHQKKQLYEKLGIQSDITFWRDIFFLYNPMIISASGNSNKSYITDNGYHHVITPEAISL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCCEEECCCEEEEECHHHHEE
ALENHEFKPWIQPVFCAQTGVLTGCEILVRWEHPQTGIIPPDQFIPLAESSGLIVILTRQ
ECCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHH
LMKQTADILLPVKDLLPDNFHIGINVSAGCFLAPGFERECLNLVNKLGTDKIKLVLELTE
HHHHHHHHEEEHHHHCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHCCCEEEEEEEECC
RNPIPVTPEARAIFDSLHRHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASID
CCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKGVHFLQGYLYSPPLPGAKFISD
HHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
WVKNSAVA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 1956285 [H]