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The map label for this gene is ycjZ [C]

Identifier: 218549989

GI number: 218549989

Start: 2752630

End: 2753523

Strand: Reverse

Name: ycjZ [C]

Synonym: EFER_2674

Alternate gene names: 218549989

Gene position: 2753523-2752630 (Counterclockwise)

Preceding gene: 218549991

Following gene: 218549985

Centisome position: 60.01

GC content: 48.55

Gene sequence:

>894_bases
ATGCTCAAAGAAAACTTCAATGAACTGCAAATCTTTCTTGTGGTGGCAAGAGAGCGAAGTTTTACCAAAGCGGCGGGCAA
ACTCGGTGTTTCTCAGTCTGCTCTCAGCCATGCAATTAAGGCACTGGAGGAAAGGTTAAATATCCGCCTCTTAACCCGAA
CGACCCGTAGCGTGGCCCCTACGGAAGCAGGGGAGAGAATAATTGCCTGTCTGGAGCCGCGTATTGATGAGCTTGAACAG
GAACTGGAATCACTTATTCAACTGAACGGCATGCCCTCCGGGAATATCCGTTTATCTGCCGGGGAACATGCCGCGCGAAG
TTTGGTCTGGCCGAAGCTAAAACCCTTTCTCAGAGAGTATCCCGAAATCAATGTCGAACTGGTGGTGGATAACGGTTTTG
TCGATATTGTTGAAGGCCGTTTTGATGCCGGGATACGATTGGGGGAAAATGTTGATAAAGATATGGTAGCGGTCAGGATT
GGACCAGATATGCGGATGGCTGTGGTGGGAGCACCGGCGTATTTTGCCGCAAACGCCTTACCTGAAACGCCATACGAGCT
ACAAAATCATCAGTGTATCAATATGCGTTTGCCAACTGCCGGTGGAATTTATCACTGGGAGTTTGAACGCGAAGGCAAAC
CTTTACGTGTCAGAGTTGATGGGCAACTGACGTTTAATCTGCTGCCAGAAAGAATTGATGCCGCATTATCAGGATTTGGG
ATCGCTTGTGTTCCTGAAGACATGGTTCAGGAGTATATAAAGTCAGGCAAGCTTATTCAGGTGTTACAGGAGTGGTGTCC
CACTTTTCCCGGATATTATCTCTACTACCCCAGCCGTAAGCAGCATCCGCCAGCTTTTGCGCTGTTGATCGATGCACTTC
GCTACTCGGAATAA

Upstream 100 bases:

>100_bases
AATGGCTCTTTTTATGACGTCTTCCGGCTAAAATGATTGTGTCTATAAACACCCTTCATGAGTACGTTTTGAAGGGAATG
TTTCTACAAGGAAACCACTA

Downstream 100 bases:

>100_bases
CGGGTCCGCATTTTGCTGCTGACCCGTGTGGATATTAACGATCTATGCGAGCCTGGTGCTCAGGCGAGTAACGTTCACCG
ACTATTTTAATTGTTTCCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEERLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQ
ELESLIQLNGMPSGNIRLSAGEHAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
GPDMRMAVVGAPAYFAANALPETPYELQNHQCINMRLPTAGGIYHWEFEREGKPLRVRVDGQLTFNLLPERIDAALSGFG
IACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYYPSRKQHPPAFALLIDALRYSE

Sequences:

>Translated_297_residues
MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEERLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQ
ELESLIQLNGMPSGNIRLSAGEHAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
GPDMRMAVVGAPAYFAANALPETPYELQNHQCINMRLPTAGGIYHWEFEREGKPLRVRVDGQLTFNLLPERIDAALSGFG
IACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYYPSRKQHPPAFALLIDALRYSE
>Mature_297_residues
MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEERLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQ
ELESLIQLNGMPSGNIRLSAGEHAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
GPDMRMAVVGAPAYFAANALPETPYELQNHQCINMRLPTAGGIYHWEFEREGKPLRVRVDGQLTFNLLPERIDAALSGFG
IACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYYPSRKQHPPAFALLIDALRYSE

Specific function: May have a role in the regulation of oprD expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787589, Length=295, Percent_Identity=48.4745762711864, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=39.2491467576792, Blast_Score=230, Evalue=8e-62,
Organism=Escherichia coli, GI1786401, Length=280, Percent_Identity=29.6428571428571, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=30.2681992337165, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789440, Length=273, Percent_Identity=28.9377289377289, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693193, Length=281, Percent_Identity=29.8932384341637, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=27.9863481228669, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=26.953125, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=27.2413793103448, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788748, Length=123, Percent_Identity=36.5853658536585, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789173, Length=270, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693105, Length=150, Percent_Identity=27.3333333333333, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787879, Length=144, Percent_Identity=30.5555555555556, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788297, Length=131, Percent_Identity=33.587786259542, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=124, Percent_Identity=30.6451612903226, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789283, Length=183, Percent_Identity=31.6939890710383, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33425; Mature: 33425

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEERLNIRLLTRTTRSVAP
CCCCCCCCEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
TEAGERIIACLEPRIDELEQELESLIQLNGMPSGNIRLSAGEHAARSLVWPKLKPFLREY
CHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHC
PEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRIGPDMRMAVVGAPAYFAANAL
CCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHCCC
PETPYELQNHQCINMRLPTAGGIYHWEFEREGKPLRVRVDGQLTFNLLPERIDAALSGFG
CCCCHHHCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCEEEEEECHHHHHHHHHCCC
IACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYYPSRKQHPPAFALLIDALRYSE
EEECCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEERLNIRLLTRTTRSVAP
CCCCCCCCEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
TEAGERIIACLEPRIDELEQELESLIQLNGMPSGNIRLSAGEHAARSLVWPKLKPFLREY
CHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHC
PEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRIGPDMRMAVVGAPAYFAANAL
CCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHCCC
PETPYELQNHQCINMRLPTAGGIYHWEFEREGKPLRVRVDGQLTFNLLPERIDAALSGFG
CCCCHHHCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCEEEEEECHHHHHHHHHCCC
IACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYYPSRKQHPPAFALLIDALRYSE
EEECCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043; 1427017 [H]