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The map label for this gene is mak [H]

Identifier: 218549945

GI number: 218549945

Start: 2702752

End: 2703804

Strand: Reverse

Name: mak [H]

Synonym: EFER_2630

Alternate gene names: 218549945

Gene position: 2703804-2702752 (Counterclockwise)

Preceding gene: 218549947

Following gene: 218549942

Centisome position: 58.92

GC content: 52.23

Gene sequence:

>1053_bases
TTGAACCACAGCATGATAATTTCCACGGCCTTGTTGTTAAATTTAGCGGGCATAATAACGAATTGTCGGCCCCCTTGCAT
TGCCAATCCCGTTTCCCGTCTCTACGCTATTGATATTGAAAATAATAAGGAGTGTACCGTGCGTATAGGTATCGATTTAG
GTGGCACCAAAACAGAAGTCATCGCGCTTGGGGAACACGGAGAGCAATGCTTTCGTCATCGCCTGCCCACGCCGCGAGAT
GACTATCAGCAAACCATAGAAACAATCGCCACATTGGTCGGGATGGCCGAAAAGGTAACCGGGCAGCGCGGAACTGTAGG
TGTTGGCATACCCGGTTCTATTTCGCCTTATACTGGAGTGGTGAAAAATGCCAATTCAACCTGGCTTAACGGGCAACCGT
TTGACAAAGATCTCAGCGTTCGCCTGCAGCGGGAAGTTCGCCTGGCGAATGATGCTAACTGCCTGGCGGTCTCTGAAGCG
GTTGATGGCGCCGCTGCTGGTGCACAAACGGTGTTTGCCGTAATTATTGGCACGGGGTGTGGCGCGGGCGTGGCTTTAAA
CGGGAGAGCGCATATTGGCGGAAACGGAACGGCGGGCGAATGGGGGCATAACCCACTGCCATGGATGGATGAAGATGAGT
TGCGCTATCGACAAGAAGTGCCTTGTTACTGCGGCAAACAGGGCTGCATTGAAACCTTTATTTCAGGTACGGGTTTTGCT
ACGGACTATCAGCGTCTGAGCGGTAAGCAACGTGAAGGCAGTGAGATTATCCAACTGGTAGAGGCAAAGGACGCTATTGC
TGAACTGGCCTTAAGCCGCTATGAGTTACGCCTGGCAAAATCGCTTGCGCATGTTGTGAATATTCTCGACCCGGATGTGA
TTGTGTTAGGCGGTGGAATGAGCAATGTGGAGCGACTGTATCAGACCGTTCCGCAGTTGATTAAGTCGTTCGTCTTTGGC
GGTGAGTGCGAGACCCCTTTGCGCAAAGCGCGTCATGGAGATTCCAGCGGTGTGCGCGGCGCGGCATGGTTATGGCCGCA
ACAAGATAAATAG

Upstream 100 bases:

>100_bases
CTGGCTACCGCAGGGGGTAAATGCCATTGAGGCAAGTTGCTTTTCCATCTCTTCGGCACGCAGCGAAACATCGCGGCTAA
GACGGTATACCATTAAATTT

Downstream 100 bases:

>100_bases
TATGAGACTGTAAAATTGCCTGATGCGCTACGTTTATCAGGCATGAACAAAGCGCACTTTGTCAGCAAACTGCTGGTTTC
AGGCTCACCCGGCGGCACGT

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNHSMIISTALLLNLAGIITNCRPPCIANPVSRLYAIDIENNKECTVRIGIDLGGTKTEVIALGEHGEQCFRHRLPTPRD
DYQQTIETIATLVGMAEKVTGQRGTVGVGIPGSISPYTGVVKNANSTWLNGQPFDKDLSVRLQREVRLANDANCLAVSEA
VDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPLPWMDEDELRYRQEVPCYCGKQGCIETFISGTGFA
TDYQRLSGKQREGSEIIQLVEAKDAIAELALSRYELRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPQLIKSFVFG
GECETPLRKARHGDSSGVRGAAWLWPQQDK

Sequences:

>Translated_350_residues
MNHSMIISTALLLNLAGIITNCRPPCIANPVSRLYAIDIENNKECTVRIGIDLGGTKTEVIALGEHGEQCFRHRLPTPRD
DYQQTIETIATLVGMAEKVTGQRGTVGVGIPGSISPYTGVVKNANSTWLNGQPFDKDLSVRLQREVRLANDANCLAVSEA
VDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPLPWMDEDELRYRQEVPCYCGKQGCIETFISGTGFA
TDYQRLSGKQREGSEIIQLVEAKDAIAELALSRYELRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPQLIKSFVFG
GECETPLRKARHGDSSGVRGAAWLWPQQDK
>Mature_350_residues
MNHSMIISTALLLNLAGIITNCRPPCIANPVSRLYAIDIENNKECTVRIGIDLGGTKTEVIALGEHGEQCFRHRLPTPRD
DYQQTIETIATLVGMAEKVTGQRGTVGVGIPGSISPYTGVVKNANSTWLNGQPFDKDLSVRLQREVRLANDANCLAVSEA
VDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPLPWMDEDELRYRQEVPCYCGKQGCIETFISGTGFA
TDYQRLSGKQREGSEIIQLVEAKDAIAELALSRYELRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPQLIKSFVFG
GECETPLRKARHGDSSGVRGAAWLWPQQDK

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=89.7350993377484, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=39.0728476821192, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1786891, Length=275, Percent_Identity=22.9090909090909, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790522, Length=331, Percent_Identity=25.6797583081571, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 37655; Mature: 37655

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSMIISTALLLNLAGIITNCRPPCIANPVSRLYAIDIENNKECTVRIGIDLGGTKTEV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEECCCCEEEEEEEEECCCCCEEE
IALGEHGEQCFRHRLPTPRDDYQQTIETIATLVGMAEKVTGQRGTVGVGIPGSISPYTGV
EEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHH
VKNANSTWLNGQPFDKDLSVRLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGC
HCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHCCHHHHHHHEEEEEEECCC
GAGVALNGRAHIGGNGTAGEWGHNPLPWMDEDELRYRQEVPCYCGKQGCIETFISGTGFA
CCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
TDYQRLSGKQREGSEIIQLVEAKDAIAELALSRYELRLAKSLAHVVNILDPDVIVLGGGM
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCH
SNVERLYQTVPQLIKSFVFGGECETPLRKARHGDSSGVRGAAWLWPQQDK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MNHSMIISTALLLNLAGIITNCRPPCIANPVSRLYAIDIENNKECTVRIGIDLGGTKTEV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEECCCCEEEEEEEEECCCCCEEE
IALGEHGEQCFRHRLPTPRDDYQQTIETIATLVGMAEKVTGQRGTVGVGIPGSISPYTGV
EEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHH
VKNANSTWLNGQPFDKDLSVRLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGC
HCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHCCHHHHHHHEEEEEEECCC
GAGVALNGRAHIGGNGTAGEWGHNPLPWMDEDELRYRQEVPCYCGKQGCIETFISGTGFA
CCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
TDYQRLSGKQREGSEIIQLVEAKDAIAELALSRYELRLAKSLAHVVNILDPDVIVLGGGM
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCH
SNVERLYQTVPQLIKSFVFGGECETPLRKARHGDSSGVRGAAWLWPQQDK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]