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The map label for this gene is phoB [H]

Identifier: 218549942

GI number: 218549942

Start: 2697416

End: 2698105

Strand: Reverse

Name: phoB [H]

Synonym: EFER_2627

Alternate gene names: 218549942

Gene position: 2698105-2697416 (Counterclockwise)

Preceding gene: 218549945

Following gene: 218549941

Centisome position: 58.8

GC content: 50.14

Gene sequence:

>690_bases
ATGGCGAGACGTATTCTGGTTGTAGAAGATGAAGCTCCAATTCGAGAAATGGTCTGCTTCGTGCTCGAGCAAAATGGCTT
TCAGCCAGTAGAAGCCGAAGATTATGACAGTGCTGTGAACCAACTTAATGAACCCTGGCCGGATCTCATTCTTCTGGACT
GGATGTTGCCAGGCGGCTCTGGTCTGCAATTTATTAAACATCTCAAACGGGAAGCGCTTACCCGGGATATTCCGGTTGTG
ATGCTGACGGCGAGAGGCGAAGAAGAAGATCGTGTACGCGGCCTTGAAACAGGCGCGGATGACTATATTACCAAACCGTT
TTCCCCGAAAGAGCTGGTGGCGCGTATTAAGGCCGTTATGCGCCGTATTTCGCCTATGGCAGTTGAAGAAGTTATTGAAA
TGCAAGGGCTAAGTCTTGATCCAACCTCACATCGGGTCATGACGGGCGAAACACCGCTGGATATGGGGCCTACCGAATTT
AAATTACTGCACTTTTTTATGACGCATCCTGAACGCGTCTACAGTCGCGAGCAGTTGCTAAATCATGTCTGGGGAACGAA
TGTGTATGTGGAAGACCGTACAGTCGATGTCCACATTCGTCGCTTGCGTAAAGCGCTGGAACCAAGCGGGCATGACCGCA
TGGTGCAGACAGTGCGAGGAACAGGGTATCGTTTTTCGACCCGCTTTTGA

Upstream 100 bases:

>100_bases
CTGTACGAAACATCGACGATCTTTTCATAAATCTGTCATAAAACTGACGCATAATACCGCCGCATTGATAACGTGTAATT
TATCTATAACAGGGCTAATC

Downstream 100 bases:

>100_bases
CGCCTCACCCGCCGTAATGGCGGGTGAGGCTTTCTATAGAGTAAATGGAGTATGACGCGTGCTTGAACGGCTGTCATGGA
AAAGGCTGGTTCTGGAACTG

Product: transcriptional regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGLQFIKHLKREALTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMTGETPLDMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPSGHDRMVQTVRGTGYRFSTRF

Sequences:

>Translated_229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGLQFIKHLKREALTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMTGETPLDMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPSGHDRMVQTVRGTGYRFSTRF
>Mature_228_residues
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGLQFIKHLKREALTRDIPVVM
LTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMTGETPLDMGPTEFK
LLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPSGHDRMVQTVRGTGYRFSTRF

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=96.943231441048, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=40.625, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.117903930131, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=38.0090497737557, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=35.4260089686099, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=35.8744394618834, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=34.0909090909091, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.8888888888889, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=34.1991341991342, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=38.4279475982533, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=32.7272727272727, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=33.7777777777778, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=31.7391304347826, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=28.3898305084746, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=29.5081967213115, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26418; Mature: 26287

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGS
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GLQFIKHLKREALTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPTSHRVMTGETPLDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEPSGHDRMVQTVRGTGYRFSTRF
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGS
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GLQFIKHLKREALTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPTSHRVMTGETPLDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEPSGHDRMVQTVRGTGYRFSTRF
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]