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The map label for this gene is brnQ [H]

Identifier: 218549939

GI number: 218549939

Start: 2694338

End: 2695657

Strand: Reverse

Name: brnQ [H]

Synonym: EFER_2624

Alternate gene names: 218549939

Gene position: 2695657-2694338 (Counterclockwise)

Preceding gene: 218549941

Following gene: 218549938

Centisome position: 58.75

GC content: 52.05

Gene sequence:

>1320_bases
ATGACCCATAAGTTAAGATCACGCGATATCATCGCATTGGGATTTATGACATTTGCGTTGTTTGTTGGCGCAGGTAACAT
CATTTTCCCTCCAATGGTTGGCCTGCAGGCTGGCGAACATGTCTGGACTGCCGCTTTCGGTTTCCTTATTACTGCTGTCG
GTCTTCCGGTATTGACAGTTGTCGCACTGGCAAAAGTTGGTGGCGGTATCGATAGTCTGAGTTCGCCCATCGGTAAAGTA
GCTGGCGTTTTGCTGGCAACAGTATGTTATCTGGCTGTTGGTCCGCTGTTTGCTACCCCGCGTACCGCAACGGTCTCCTT
TGAAGTGGGTATTGCTCCGCTGACGGGTAACTCACCGCTGCCATTATTTATCTACAGCCTGATTTACTTTGCTATCGTGA
TTCTGGTTTCGCTTTATCCGGGCAAACTGCTGGATACCGTCGGTAACTTCCTGGCACCGCTGAAAATTATCGCGCTGGTC
ATCCTGTCTGTTGCCGCAATTGTCTGGCCGGCGGGCTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCCGCGTTTTC
TAACGGCTTCGTTAATGGCTATCTGACCATGGATACGCTTGGCGCGATGGTGTTTGGTATCGTTATTGTTAATGCGGCGC
GTTCTCGCGGCGTATCCGAAGCACGTTTGTTGACTCGTTACACCGTTTGGGCTGGTCTGATGGCGGGTGTTGGTCTGACA
TTGCTGTATCTGGCGTTGTTCCGTTTGGGTTCCGACAGTGCATCGCTGCTTGAACAGTCAGCTAATGGTGCGGCTATTCT
GCATGCCTACGTTCAGCATACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTTATCGCCTGCCTGGTGA
CGGCGGTTGGCCTGACGTGTGCTTGTGCAGAGTTTTTCGCTCAATATTTACCGCTCTCTTACCGGACACTGGTGTTTATC
CTTGGCGGTTTCTCGATGGTGGTATCTAACCTCGGTTTGAGCCAGCTGATTCAGATCTCTGTTCCAGTGCTGACAGCAAT
TTATCCGCCATGTATCGCACTGGTTGTATTAAGCTTCACCCGCTCCTGGTGGCATAATTCCACCCGCGTAATTGCACCGC
CTATGTTTATCAGCCTGGTTTTTGGTATCCTTGACGGCATTAAAGCCTCGGCAATCGGTGATGTATTACCTGCCTGGGTG
CAGCGTCTGCCGCTGGCAGAACAAGGGTTGGCGTGGTTAATTCCAACAGTGGTGATGGTTGTTCTGGCCATTATCTGGGA
TCGTGCAGCAGGGCGTCAGGTGACCTCCAGCGCTCACTAA

Upstream 100 bases:

>100_bases
AGCATATCCCGCTATAATTGATTAAAAATGCTGCACTATCATCCCGTTGGTAAAGCATGAATTTACGTATCAACACCACA
TCCACAGGCAGTAAGATTTT

Downstream 100 bases:

>100_bases
ATCACTGACAATGTGTTTTTGACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATCGATGG
AAAGTAACAACAAGCTAAAG

Product: branched chain amino acid transporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTHKLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTVVALAKVGGGIDSLSSPIGKV
AGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGNSPLPLFIYSLIYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLT
LLYLALFRLGSDSASLLEQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTCACAEFFAQYLPLSYRTLVFI
LGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSTRVIAPPMFISLVFGILDGIKASAIGDVLPAWV
QRLPLAEQGLAWLIPTVVMVVLAIIWDRAAGRQVTSSAH

Sequences:

>Translated_439_residues
MTHKLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTVVALAKVGGGIDSLSSPIGKV
AGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGNSPLPLFIYSLIYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLT
LLYLALFRLGSDSASLLEQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTCACAEFFAQYLPLSYRTLVFI
LGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSTRVIAPPMFISLVFGILDGIKASAIGDVLPAWV
QRLPLAEQGLAWLIPTVVMVVLAIIWDRAAGRQVTSSAH
>Mature_438_residues
THKLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTVVALAKVGGGIDSLSSPIGKVA
GVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGNSPLPLFIYSLIYFAIVILVSLYPGKLLDTVGNFLAPLKIIALVI
LSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLTL
LYLALFRLGSDSASLLEQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTCACAEFFAQYLPLSYRTLVFIL
GGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSTRVIAPPMFISLVFGILDGIKASAIGDVLPAWVQ
RLPLAEQGLAWLIPTVVMVVLAIIWDRAAGRQVTSSAH

Specific function: Component of the LIV-II transport system for branched- chain amino acids. This LIV-II transport system may be H(+)- coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=439, Percent_Identity=95.8997722095672, Blast_Score=838, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46192; Mature: 46061

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHKLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTV
CCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALAKVGGGIDSLSSPIGKVAGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGNSPL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCH
PLFIYSLIYFAIVILVSLYPGKLLDTVGNFLAPLKIIALVILSVAAIVWPAGSISTATEA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
YQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLT
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLYLALFRLGSDSASLLEQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTC
HHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ACAEFFAQYLPLSYRTLVFILGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSWWHNSTRVIAPPMFISLVFGILDGIKASAIGDVLPAWVQRLPLAEQGLAWLIPTVVMV
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHH
VLAIIWDRAAGRQVTSSAH
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
THKLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTV
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALAKVGGGIDSLSSPIGKVAGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGNSPL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCH
PLFIYSLIYFAIVILVSLYPGKLLDTVGNFLAPLKIIALVILSVAAIVWPAGSISTATEA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
YQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLT
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLYLALFRLGSDSASLLEQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTC
HHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ACAEFFAQYLPLSYRTLVFILGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSWWHNSTRVIAPPMFISLVFGILDGIKASAIGDVLPAWVQRLPLAEQGLAWLIPTVVMV
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHH
VLAIIWDRAAGRQVTSSAH
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]