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The map label for this gene is entE [H]

Identifier: 218549818

GI number: 218549818

Start: 2561685

End: 2563295

Strand: Reverse

Name: entE [H]

Synonym: EFER_2500

Alternate gene names: 218549818

Gene position: 2563295-2561685 (Counterclockwise)

Preceding gene: 218549819

Following gene: 218549817

Centisome position: 55.86

GC content: 55.93

Gene sequence:

>1611_bases
ATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCACT
GACCGACATTCTGACTCGCCACGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGGCAGTTGAGTTACCGGGAGC
TGAATCAGGCGGCGGATAACCTCGCCTGTAGTTTGCGCCGTCAGGGCATTAAACCCGGTGAAACCGCGCTGGTACAACTG
GGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGCTGTTCAGTCA
TCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCGGCGTTACTGATTGCCGATCGCCAACATGCGCTATTTA
GCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCCATTCGCGTGGTGCAACTGCTGAACGACAGCGGTGAG
CATAACTTGCAGGATGCGATTAACCATCCGGCTGAGGATTTTACTGCCACGCCATCTCCTGCTGATGAAGTGGCCTATTT
CCAGCTTTCCGGCGGCACCACCGGCACACCGAAACTGATCCCGCGCACTCATAACGACTACTACTACAGCGTGCGTCGTA
GCGTCGAGATTTGTCAGTTCACACAACAGACGCGCTACCTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCG
CCGGGATCGTTGGGGGTATTTCTCGCTGGCGGCACTGTCGTTCTGGCTGCCGACCCCAGCGCCACGCTTTGTTTCCCATT
GATTGAAAAACATCAGGTGAACGTCACCGCGCTGGTGCCGCCAGCAGTCAGCCTGTGGTTACAGGCGCTGACCGAAGGTG
AAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGTGGCGCACGTCTTTCTGCCACGCTTGCGGCGCGTATT
CCCGCAGAGATTGGCTGCCAGTTACAGCAGGTATTCGGCATGGCGGAAGGGCTGGTGAACTACACCCGTCTTGATGATAG
CGCGGAGAAAATTATCCATACCCAGGGCTATCCAATGTGTCCGGATGATGAAGTGTGGGTTGCCGATGCTGACGGAAATC
CACTGCCGCAAGGGGAGGTTGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCAC
AATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGGTACATCACCGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGTTACTGCGCC
ATCCGGCGGTGATCTACGCCGCCCTGGTGAGTATGGAAGATGAGCTGATGGGCGAAAAAAGCTGTGCTTATCTGGTGGTA
AAAGAACCACTGCGTGCGGTACAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGT
GGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCG
CATCAGCCTGA

Upstream 100 bases:

>100_bases
GGGGATTGTGCCTGCGTCCTCACCGTTGGGTGAATGGCGCGAAACGGGCGTCAAACTTTCTACCATGTTGAACGTTTTTG
GATTGCATTAAGGAGCGAGG

Downstream 100 bases:

>100_bases
AGGAGAGAACACGATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCACAATAAAGTCA
ACTGGACCTTTGAGCCGCAA

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA

Sequences:

>Translated_536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
>Mature_535_residues
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQLG
NVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEH
NLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSSP
GSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHN
ASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVVK
EPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=23.960396039604, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=23.921568627451, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI42544132, Length=522, Percent_Identity=22.7969348659004, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157311624, Length=518, Percent_Identity=23.5521235521236, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI157311622, Length=518, Percent_Identity=23.5521235521236, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI156151445, Length=368, Percent_Identity=24.7282608695652, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI58082049, Length=518, Percent_Identity=23.9382239382239, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI187761345, Length=410, Percent_Identity=24.390243902439, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI187761343, Length=410, Percent_Identity=24.390243902439, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI115511026, Length=511, Percent_Identity=22.5048923679061, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI28416953, Length=530, Percent_Identity=21.5094339622642, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI57165412, Length=396, Percent_Identity=22.7272727272727, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI57165410, Length=396, Percent_Identity=21.969696969697, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI8923896, Length=554, Percent_Identity=20.2166064981949, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI253970489, Length=381, Percent_Identity=23.0971128608924, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=99.4402985074627, Blast_Score=1103, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=31.203007518797, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=26.4765784114053, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788107, Length=537, Percent_Identity=25.1396648044693, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=23.4200743494424, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786801, Length=562, Percent_Identity=25.2669039145907, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786529, Length=528, Percent_Identity=23.2954545454545, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789201, Length=359, Percent_Identity=22.0055710306407, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788595, Length=507, Percent_Identity=23.2741617357002, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=539, Percent_Identity=26.7161410018553, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=544, Percent_Identity=26.2867647058824, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=23.6111111111111, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=525, Percent_Identity=24.1904761904762, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=519, Percent_Identity=23.8921001926782, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=519, Percent_Identity=23.8921001926782, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=519, Percent_Identity=23.8921001926782, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=23.9171374764595, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=24.2063492063492, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=27.0949720670391, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=369, Percent_Identity=23.3062330623306, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=531, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=521, Percent_Identity=23.9923224568138, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=539, Percent_Identity=22.8200371057514, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=544, Percent_Identity=23.1617647058824, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=539, Percent_Identity=22.8200371057514, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=540, Percent_Identity=22.4074074074074, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24581924, Length=524, Percent_Identity=23.6641221374046, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922652, Length=497, Percent_Identity=24.1448692152917, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=23.1499051233397, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=24.1509433962264, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356947, Length=388, Percent_Identity=24.2268041237113, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI18859661, Length=367, Percent_Identity=24.2506811989101, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648260, Length=272, Percent_Identity=27.2058823529412, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667955, Length=564, Percent_Identity=22.8723404255319, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=22.7777777777778, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24667959, Length=374, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356441, Length=443, Percent_Identity=21.4446952595937, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648255, Length=499, Percent_Identity=22.6452905811623, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648253, Length=499, Percent_Identity=22.4448897795591, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=22.6452905811623, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130357, Length=448, Percent_Identity=22.5446428571429, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281365686, Length=329, Percent_Identity=25.531914893617, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648257, Length=376, Percent_Identity=24.468085106383, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 59057; Mature: 58925

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEHNLQDAINHPAEDFTATPSP
CEEEEECCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCHHHCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALTEGESRAQLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHCCCCEEEEECCHHHHHHHHHHHCCHHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
PDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEHNLQDAINHPAEDFTATPSP
CEEEEECCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCHHHCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALTEGESRAQLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHCCCCEEEEECCHHHHHHHHHHHCCHHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
PDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]