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The map label for this gene is tolA [H]

Identifier: 218549688

GI number: 218549688

Start: 2420241

End: 2421452

Strand: Reverse

Name: tolA [H]

Synonym: EFER_2365

Alternate gene names: 218549688

Gene position: 2421452-2420241 (Counterclockwise)

Preceding gene: 218549689

Following gene: 218549687

Centisome position: 52.77

GC content: 49.01

Gene sequence:

>1212_bases
GTGTCAAAGGCAACCGAACAAAACGACAAACTTAAACGAGCGATAATTATTTCAGCAGTGCTGCATGTCATTTTATTTGC
AGCGCTGATCTGGAGCTCGTTTGATGAAAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCTATCGACGCGGTAA
TGGTTGATACTGGTGCTGTTGTAGCAAACAGCGAGCGTATCAAGGAGCAGGAAGCCAGTGCGCGTCGTTCCGAAGAGCAA
CGTAAGAAAAAGGAACAACAAGCGGCTGAAGAACTCCGTGAAAGACAAGCCGCAGAGCAGGAACGGTTAAAACAGGCTGA
AAAAGAGCGTATAGCCGCTCAGCAACAGAAAAAACAAGCTGAAGAGGACGCAAAACAGGCAGAGTTAAAGCGTCAGCAAG
CTGAAGAGGCCATGAAGAAAGCAGAAGCACAGAAAAAGGCTGAGGCAGAGGCAGCTAAACTCGCCGAGGCAGAGGCGCAG
AAAAAAGCTATAGCAGAAAAAGCAATAGCAGATAAAAAAGCTGCTGAGAAAGCCGCTGCAGAAAAAGCAGCAGCAGAGAA
AAAGGCCGCTGAGAAAGCCGCTGCAGACAAAGCAGCAGCAGAGAAAAAGGCTGCTGAGAAAGCCGCTGCAGACAAAGCAG
CAGCAGAGAAAAAAGCTGCCGAGAAAGCCGCTGCAGACAAAGCAGCAGCAGAGAAAAAAGCTGCTGAGAAAGCCGCTGCA
GACAAAGCAGCAGCAGAGAAAAAAGCCGCCGAGAAAGCCGCAGCAGAGAAAAAGGCTGCCGAAAAAGCAGCGGCAGACAA
AAAGGCTGCCCAGAAAGCAGCAGAGGCTGATGATATTTTTGGTGAGCTAAGTTCTGGTAAGAATGCACCGAAAACGGGCG
GTGGGCCGAAAGGAGGCCATGATTCACCTGCTGGGGTGGGTAATACTAAAAATAATGGCGCATCAGGCGAGGATATCAAT
AACTATGCCGGGCAAATTAAATCTGCTATTGAAAGCAGGTTTTATGACGCATCTCTTTATGCAGGTAAAACCTGTACATT
GCGTATAAAACTGGCCCCGGATGGCATGTTACTGGATATTAAGCCGGAAGGTGGAGATCCTGCGCTTTGTCAGGCTGCGC
TTGCTGCAGCTAAACTTGCGAAGATCCCAAAACCACCAAGCCAGGCGGTATATGAAGTGTTCAAAAATGCTCCACTGGAC
TTTAAGCCGTAA

Upstream 100 bases:

>100_bases
GTGTGAAATCGGTTGGCTTAATGACGCAGCCTATCTAATTACCTGCGTTTCCAGGCGTAAAGATAGCGAGTTACGGGCGA
ACAGTTTTTGGGAACCAAGA

Downstream 100 bases:

>100_bases
TGTTGTTTTTCTCTGGTATAACGAAGAAGAACAAATAGGGCGGGTCGCTGGGTTCTGGTAGTTTTGTGTATTTGAGTTTG
TTAAAATTCTGCTAAATTAT

Product: TolA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGGGGGSSIDAVMVDTGAVVANSERIKEQEASARRSEEQ
RKKKEQQAAEELRERQAAEQERLKQAEKERIAAQQQKKQAEEDAKQAELKRQQAEEAMKKAEAQKKAEAEAAKLAEAEAQ
KKAIAEKAIADKKAAEKAAAEKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAA
DKAAAEKKAAEKAAAEKKAAEKAAADKKAAQKAAEADDIFGELSSGKNAPKTGGGPKGGHDSPAGVGNTKNNGASGEDIN
NYAGQIKSAIESRFYDASLYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLD
FKP

Sequences:

>Translated_403_residues
MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGGGGGSSIDAVMVDTGAVVANSERIKEQEASARRSEEQ
RKKKEQQAAEELRERQAAEQERLKQAEKERIAAQQQKKQAEEDAKQAELKRQQAEEAMKKAEAQKKAEAEAAKLAEAEAQ
KKAIAEKAIADKKAAEKAAAEKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAA
DKAAAEKKAAEKAAAEKKAAEKAAADKKAAQKAAEADDIFGELSSGKNAPKTGGGPKGGHDSPAGVGNTKNNGASGEDIN
NYAGQIKSAIESRFYDASLYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLD
FKP
>Mature_402_residues
SKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGGGGGSSIDAVMVDTGAVVANSERIKEQEASARRSEEQR
KKKEQQAAEELRERQAAEQERLKQAEKERIAAQQQKKQAEEDAKQAELKRQQAEEAMKKAEAQKKAEAEAAKLAEAEAQK
KAIAEKAIADKKAAEKAAAEKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAAD
KAAAEKKAAEKAAAEKKAAEKAAADKKAAQKAAEADDIFGELSSGKNAPKTGGGPKGGHDSPAGVGNTKNNGASGEDINN
YAGQIKSAIESRFYDASLYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDF
KP

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=129, Percent_Identity=86.8217054263566, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 42107; Mature: 41976

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGGGGGSSIDAVMVDTGAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCH
VANSERIKEQEASARRSEEQRKKKEQQAAEELRERQAAEQERLKQAEKERIAAQQQKKQA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEDAKQAELKRQQAEEAMKKAEAQKKAEAEAAKLAEAEAQKKAIAEKAIADKKAAEKAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKAAAEKKAAEKAAAEKKAAEKAAADKKAAQKAAEADDIFGELSSGKNAPKTGGGPKGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DSPAGVGNTKNNGASGEDINNYAGQIKSAIESRFYDASLYAGKTCTLRIKLAPDGMLLDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEE
KPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGGGGGSSIDAVMVDTGAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCH
VANSERIKEQEASARRSEEQRKKKEQQAAEELRERQAAEQERLKQAEKERIAAQQQKKQA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEDAKQAELKRQQAEEAMKKAEAQKKAEAEAAKLAEAEAQKKAIAEKAIADKKAAEKAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAADKAAAEKKAAEKAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKAAAEKKAAEKAAAEKKAAEKAAADKKAAQKAAEADDIFGELSSGKNAPKTGGGPKGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DSPAGVGNTKNNGASGEDINNYAGQIKSAIESRFYDASLYAGKTCTLRIKLAPDGMLLDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEE
KPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]