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The map label for this gene is yeiE [H]

Identifier: 218549570

GI number: 218549570

Start: 2301334

End: 2302203

Strand: Reverse

Name: yeiE [H]

Synonym: EFER_2242

Alternate gene names: 218549570

Gene position: 2302203-2301334 (Counterclockwise)

Preceding gene: 218549574

Following gene: 218549569

Centisome position: 50.17

GC content: 51.26

Gene sequence:

>870_bases
ATGCACATCACTCTGCGGCAGTTAGAAGTATTCACTGAAGTTTTAAAAAGCGGATCAACGACTCAGGCGTCGGCAATGCT
GGCGTTATCACAGTCGGCGGTAAGCGCGGCCTTAACCGATCTGGAAGGGCAGCTTGGTGTTCAGCTCTTTGACCGTGTCG
GTAAGCGACTAGTGGTTAATGAACACGGGCGTTTGCTCTACCCGCGTGCGCTGGCATTGCTGGAGCGGGCAATTGAGATC
GAACAATTATTTCGTGGCGACAACGGTGCGATCCGTATATTTGCCAGTAGTACTATTGGCAACTATATATTGCCGGAGGT
AATTGCTCGCTATCGTCAGGACTACCCGGAGTTACCGCTGGAGCTGAGCGTTGGCAATAGCCAGGAGGTGATCCAGGCCG
TACTCGATTTTCGTGTTGATATCGGTCTGATTGAAGGTCCGTGCCACAGTACCGAGATAATTTCGGAACCGTGGCTGGAA
GACGAACTTGTCGTTTTTGCCTCCCCGTCATCACCGTTAGCTCAAGGTCCGGTAACATTAGAACAACTGGCTAATGCGCC
ATGGATCCTGCGTGAACGCGGTTCAGGAACACGGGAGATTGTTGATTATCTGTTGTTATCGCATCTGCCGAAGTTCCAGA
TGGCAATGGAGCTTGGCAACTCAGAAGCCATTAAGCACGCCGTGCGTCATGGTCTGGGAATCAGCTGCTTGTCACGGCGT
GTAATTGAAGAACAATTGCAGGCTGGCACGCTAAGTGAAGTGGCAGTGCCGTTGCCACGCCTGATGCGCACTATGTGGCG
CATTCATCATCGGCAGAAACACCTTTCAAATGCGCTACAACGGTTTTTAAGTTATTGCACCAAACCTTGA

Upstream 100 bases:

>100_bases
TAACCTTCTCCTTTATCCGGGCATTAAGGTTACGGCGATCTGGTTTAAAAATAAAATTGATTATATATTTATAATTAATC
TTTATAAGTGGTAAGCGATC

Downstream 100 bases:

>100_bases
TGTGGAGCGGGGCATGTTGAAGCAGGGGGGCGACTCACGTAATCTCTGCTTTACTTATAATCCGCAGGCAGTCATGAAGG
TCTCTTATAACTGCGTATTT

Product: DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MHITLRQLEVFTEVLKSGSTTQASAMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLERAIEI
EQLFRGDNGAIRIFASSTIGNYILPEVIARYRQDYPELPLELSVGNSQEVIQAVLDFRVDIGLIEGPCHSTEIISEPWLE
DELVVFASPSSPLAQGPVTLEQLANAPWILRERGSGTREIVDYLLLSHLPKFQMAMELGNSEAIKHAVRHGLGISCLSRR
VIEEQLQAGTLSEVAVPLPRLMRTMWRIHHRQKHLSNALQRFLSYCTKP

Sequences:

>Translated_289_residues
MHITLRQLEVFTEVLKSGSTTQASAMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLERAIEI
EQLFRGDNGAIRIFASSTIGNYILPEVIARYRQDYPELPLELSVGNSQEVIQAVLDFRVDIGLIEGPCHSTEIISEPWLE
DELVVFASPSSPLAQGPVTLEQLANAPWILRERGSGTREIVDYLLLSHLPKFQMAMELGNSEAIKHAVRHGLGISCLSRR
VIEEQLQAGTLSEVAVPLPRLMRTMWRIHHRQKHLSNALQRFLSYCTKP
>Mature_289_residues
MHITLRQLEVFTEVLKSGSTTQASAMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLERAIEI
EQLFRGDNGAIRIFASSTIGNYILPEVIARYRQDYPELPLELSVGNSQEVIQAVLDFRVDIGLIEGPCHSTEIISEPWLE
DELVVFASPSSPLAQGPVTLEQLANAPWILRERGSGTREIVDYLLLSHLPKFQMAMELGNSEAIKHAVRHGLGISCLSRR
VIEEQLQAGTLSEVAVPLPRLMRTMWRIHHRQKHLSNALQRFLSYCTKP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=286, Percent_Identity=92.3076923076923, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI87082132, Length=288, Percent_Identity=27.4305555555556, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=30.7377049180328, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788748, Length=245, Percent_Identity=28.9795918367347, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787601, Length=198, Percent_Identity=29.2929292929293, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786401, Length=306, Percent_Identity=26.1437908496732, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787879, Length=300, Percent_Identity=26.3333333333333, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI157672245, Length=206, Percent_Identity=30.5825242718447, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787806, Length=259, Percent_Identity=25.8687258687259, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789204, Length=246, Percent_Identity=28.4552845528455, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI145693105, Length=291, Percent_Identity=24.3986254295533, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=25.3012048192771, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789506, Length=197, Percent_Identity=27.9187817258883, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789639, Length=146, Percent_Identity=30.1369863013699, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=23.6, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=213, Percent_Identity=28.169014084507, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=240, Percent_Identity=22.0833333333333, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32261; Mature: 32261

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITLRQLEVFTEVLKSGSTTQASAMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVN
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEC
EHGRLLYPRALALLERAIEIEQLFRGDNGAIRIFASSTIGNYILPEVIARYRQDYPELPL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCE
ELSVGNSQEVIQAVLDFRVDIGLIEGPCHSTEIISEPWLEDELVVFASPSSPLAQGPVTL
EEECCCHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCHH
EQLANAPWILRERGSGTREIVDYLLLSHLPKFQMAMELGNSEAIKHAVRHGLGISCLSRR
HHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VIEEQLQAGTLSEVAVPLPRLMRTMWRIHHRQKHLSNALQRFLSYCTKP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHITLRQLEVFTEVLKSGSTTQASAMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVN
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEC
EHGRLLYPRALALLERAIEIEQLFRGDNGAIRIFASSTIGNYILPEVIARYRQDYPELPL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCE
ELSVGNSQEVIQAVLDFRVDIGLIEGPCHSTEIISEPWLEDELVVFASPSSPLAQGPVTL
EEECCCHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCHH
EQLANAPWILRERGSGTREIVDYLLLSHLPKFQMAMELGNSEAIKHAVRHGLGISCLSRR
HHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VIEEQLQAGTLSEVAVPLPRLMRTMWRIHHRQKHLSNALQRFLSYCTKP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]