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The map label for this gene is yeiT [H]

Identifier: 218549559

GI number: 218549559

Start: 2286860

End: 2288098

Strand: Direct

Name: yeiT [H]

Synonym: EFER_2231

Alternate gene names: 218549559

Gene position: 2286860-2288098 (Clockwise)

Preceding gene: 218549558

Following gene: 218549560

Centisome position: 49.84

GC content: 51.09

Gene sequence:

>1239_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGCCTTTACTGGCAATAAAAGAGGCATCCCGCTGTTT
ATTATGTCATGACGCCCCCTGTAGTCAGGCTTGTCCGGCGCAAACTGACCCAGGGAAATTTATCCGCTCAATCTACTTTC
GTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGTCCAACGGAG
AAATTATGTCAAAGCGGTTGTACCCGTGCCGGTGTTGATGATCCAATTGATATTGGCCGCTTGCAGCGTTTTATCACTGA
TTTTGAACAACAAACCGGGATGGATATTTATCAGCCAGGCACTAAAACGCTCGGTAAGGTAGCGATTATTGGCGCGGGCC
CTGCCGGGTTACAGGCCAGTGTGACGCTGACAAATATGGGTTACGACGTCACTATTTATGAAAAAGAACCACAGCCCGGT
GGCTGGCTATGTAACGGTATTCCGCAATTTCGGTTACCGCAATCGGTGCTGGATGCAGAAATCGCCCGTATTGAAAAAAT
GGGCGTGACCATTAAGTGCAACAACGAAGTCGGTAAAACAATCACCCTGGAGCAACTAAAAGCGGAAAACCGCGCAGTAC
TTGTCACCGTAGGGTTGTCGGGCGGTTCCGGGCTACCGCTGTTTGAGCATAGCGACGTTGAGATTGCCGTCGACTTCTTG
CAACGTGCACGACAGGCGCAGGGCGATATCAGCATTCCACAAAGCGCATTAATTATCGGCGGCGGTGATGTCGCGATGGA
CGTTGCCAGCACGCTGAAAGTTCTCGGCTGTCAGGCGGTAACCTGCGTAGCACGTGAAGAGTTAGATGCGTTTCCGGCAA
GCGAAAAAGAGTTTGCCAGCGCCCGGGAACTGGGCGTTTCGATCATCGATGGATTCACACCAGTAGCCGTGGAAGCCAAT
AAAGTCACGTTTAAGCATGTACGGTTACCGGGCGAACTGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGCCAGCA
TGCCAGACTGGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGC
AAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCAGTGAAAACCGGGAAAGAAGCCGCCGGG
GCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAA

Upstream 100 bases:

>100_bases
TGACTGGGGTGAGCGAACGCAGACGCAGCACATGCAACTTGAAGTATGACGAGTATATTTAGCGTTCGCCTTAATTACTC
ATAGCATTAAGGAAGATCAC

Downstream 100 bases:

>100_bases
CGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGC
TATGAGATGTGCGCCAAAGC

Product: oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDDPIDIGRLQRFITDFEQQTGMDIYQPGTKTLGKVAIIGAGPAGLQASVTLTNMGYDVTIYEKEPQPG
GWLCNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGKTITLEQLKAENRAVLVTVGLSGGSGLPLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDAFPASEKEFASARELGVSIIDGFTPVAVEAN
KVTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAG
AIHHYLEGACSC

Sequences:

>Translated_412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDDPIDIGRLQRFITDFEQQTGMDIYQPGTKTLGKVAIIGAGPAGLQASVTLTNMGYDVTIYEKEPQPG
GWLCNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGKTITLEQLKAENRAVLVTVGLSGGSGLPLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDAFPASEKEFASARELGVSIIDGFTPVAVEAN
KVTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAG
AIHHYLEGACSC
>Mature_411_residues
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQSGCTRAGVDDPIDIGRLQRFITDFEQQTGMDIYQPGTKTLGKVAIIGAGPAGLQASVTLTNMGYDVTIYEKEPQPGG
WLCNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGKTITLEQLKAENRAVLVTVGLSGGSGLPLFEHSDVEIAVDFLQ
RARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDAFPASEKEFASARELGVSIIDGFTPVAVEANK
VTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAGA
IHHYLEGACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=439, Percent_Identity=31.4350797266515, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=47.7777777777778, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1788468, Length=412, Percent_Identity=96.1165048543689, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=449, Percent_Identity=32.739420935412, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1789606, Length=442, Percent_Identity=32.1266968325792, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87082180, Length=449, Percent_Identity=30.2895322939866, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1789243, Length=402, Percent_Identity=29.3532338308458, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71984108, Length=441, Percent_Identity=32.8798185941043, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=452, Percent_Identity=26.9911504424779, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320030, Length=458, Percent_Identity=25.3275109170306, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=30.8755760368664, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=30.8755760368664, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24665547, Length=445, Percent_Identity=28.7640449438202, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24665543, Length=445, Percent_Identity=28.7640449438202, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI28574881, Length=445, Percent_Identity=28.7640449438202, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24665539, Length=445, Percent_Identity=28.7640449438202, Blast_Score=135, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 44199; Mature: 44068

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDDPIDIGRLQRFITDFEQQTGMDIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCC
TKTLGKVAIIGAGPAGLQASVTLTNMGYDVTIYEKEPQPGGWLCNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEEECCEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEVGKTITLEQLKAENRAVLVTVGLSGGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHCCCCCEEEEEEEECCCCCCCCEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDAFPASEKEFAS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ARELGVSIIDGFTPVAVEANKVTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQR
HHHHCHHHHCCCCCEEEECCCEEEEEEECCCCHHHHHCEEEEEECCCCCCCHHHHCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAGAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDDPIDIGRLQRFITDFEQQTGMDIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCC
TKTLGKVAIIGAGPAGLQASVTLTNMGYDVTIYEKEPQPGGWLCNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEEECCEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEVGKTITLEQLKAENRAVLVTVGLSGGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHCCCCCEEEEEEEECCCCCCCCEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDAFPASEKEFAS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ARELGVSIIDGFTPVAVEANKVTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQR
HHHHCHHHHCCCCCEEEECCCEEEEEEECCCCHHHHHCEEEEEECCCCCCCHHHHCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAGAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]