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The map label for this gene is pbpG

Identifier: 218549549

GI number: 218549549

Start: 2278502

End: 2279434

Strand: Reverse

Name: pbpG

Synonym: EFER_2221

Alternate gene names: 218549549

Gene position: 2279434-2278502 (Counterclockwise)

Preceding gene: 218549550

Following gene: 218549547

Centisome position: 49.67

GC content: 51.13

Gene sequence:

>933_bases
ATGCCGAAATTTCGAGTTTCTTTATTTAGCCTGGCCCTGATGCTGGCTGTGCCTTTTGCACCACAGGCCGTTGCGAAAAC
GGCAGCCGCTACCACCGCTTCACAGCCGGAAATTGCCTCCGGTAGCGCGATGATTGTTGATCTGAATACCAACAAAGTGA
TCTATTCGAACCACCCGGATCTGGTGCGTCCGATTGCGTCTATCAGTAAATTAATGACCGCGATGGTCGTGCTGGATGCG
CGCCTGCCGCTGGATGAAAAACTAAAAGTGGATATCAGCCAGACGCCGGAGATGAAAGGGGTCTATTCGCGCGTACGACT
GAACAGCGAAATCAGCCGTAAAGATATGCTGTTGCTGGCGCTGATGTCTTCAGAAAACCGCGCGGCGGCAAGCCTTGCGC
ACCATTATCCCGGTGGCTACAAAGCCTTTATTAAGGCGATGAATGCGAAAGCGAAATCGCTCGGAATGAACAACACGCGC
TTTGTTGAACCGACCGGATTGTCGGTGCATAACGTTTCAACTGCCCGTGACTTAACCAAACTGCTCATTGCCAGCAAACA
ATATCCGTTGATTGGTCAGTTAAGTACTACCAGGGAAGATATGGCAACCTTCTCTAACCCGACGTATACGCTGCCGTTCC
GCAATACTAACCATCTGGTGTATCGCGATAACTGGAATATTCAGTTAACCAAAACTGGCTTTACCAATGCGGCGGGCCAT
TGTCTGGTGATGCGCACGGTTATCAATAATAAACCGGTGGCACTGGTAGTGATGGACGCGTTTGGCAAATATACCCATTT
TGCCGATGCCAGCCGCCTGCGTACCTGGATTGAGACCGGTAAAGTGATGCCTGTTCCGGCAGCAGCGTTGAGCTATAAAA
AACAAAAAGCCGCTCAGATGGCGGCGGCGGGGCAGACGGCACAGAACGATTAA

Upstream 100 bases:

>100_bases
TCGCACTTTTTGCTCGTTATGATGGCGTCGCTAGCCTCAGTAAATCCTTACTCCGGCGGTGCGCAACCCGTGCGCGTGAA
CCACTATCTGAATGCTCATC

Downstream 100 bases:

>100_bases
GTGAAGTACGGATGGCGATACTCTGCCATCCGTAATTTTTATTTCACTTCCTGCCAGTTTTTCCGCTTACTGGTTTTACC
AATCCCCGGATTCATACTGT

Product: D-alanyl-D-alanine endopeptidase

Products: NA

Alternate protein names: DD-endopeptidase; Penicillin-binding protein 7; PBP-7

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDA
RLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGH
CLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND

Sequences:

>Translated_310_residues
MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDA
RLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGH
CLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND
>Mature_309_residues
PKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDAR
LPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRF
VEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGHC
LVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND

Specific function: Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyze the DD- diaminopimelate-alanine bonds in high-molecular-mass murein sacculi

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family

Homologues:

Organism=Escherichia coli, GI87082054, Length=310, Percent_Identity=100, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1787062, Length=270, Percent_Identity=28.5185185185185, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082022, Length=273, Percent_Identity=26.3736263736264, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786851, Length=263, Percent_Identity=27.3764258555133, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBP7_ECOLI (P0AFI5)

Other databases:

- EMBL:   U00007
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X01067
- PIR:   E64981
- RefSeq:   AP_002731.1
- RefSeq:   NP_416638.4
- ProteinModelPortal:   P0AFI5
- SMR:   P0AFI5
- DIP:   DIP-48108N
- STRING:   P0AFI5
- MEROPS:   S11.002
- EnsemblBacteria:   EBESCT00000005073
- EnsemblBacteria:   EBESCT00000017044
- GeneID:   946662
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5355
- KEGG:   eco:b2134
- EchoBASE:   EB1952
- EcoGene:   EG12015
- eggNOG:   COG1686
- GeneTree:   EBGT00050000011015
- HOGENOM:   HBG728991
- OMA:   VENEMSA
- ProtClustDB:   PRK11669
- BioCyc:   EcoCyc:EG12015-MONOMER
- BioCyc:   MetaCyc:EG12015-MONOMER
- Genevestigator:   P0AFI5
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR018044
- InterPro:   IPR001967
- PRINTS:   PR00725

Pfam domain/function: PF00768 Peptidase_S11; SSF56601 PBP_transp_fold

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33888; Mature: 33757

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: ACT_SITE 67-67 ACT_SITE 70-70 ACT_SITE 124-124 BINDING 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPD
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCH
LVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHEEEEE
LMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARDLTK
EECCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCEEECCCCCEEECCHHHHHHHH
LLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGH
HHHHCCCCCEEECCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCCCCC
CLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQM
EEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AAAGQTAQND
HHCCCCCCCC
>Mature Secondary Structure 
PKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPD
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCH
LVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHEEEEE
LMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARDLTK
EECCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCEEECCCCCEEECCHHHHHHHH
LLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGH
HHHHCCCCCEEECCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCCCCC
CLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQM
EEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AAAGQTAQND
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 6386470; 7721700; 7925376