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The map label for this gene is manC2 [H]

Identifier: 218549446

GI number: 218549446

Start: 2142867

End: 2144324

Strand: Reverse

Name: manC2 [H]

Synonym: EFER_2115

Alternate gene names: 218549446

Gene position: 2144324-2142867 (Counterclockwise)

Preceding gene: 218549447

Following gene: 218549445

Centisome position: 46.73

GC content: 36.63

Gene sequence:

>1458_bases
ATGTCAAAAATGTCTGATGTACCGTTGATTGCTGTTGTAATGGCTGGTGGTACGGGTAGCCGTCTTTGGCCCCTCTCAAG
AGAGCACTATCCAAAGCAATTTCTGCAATTATCAGGTGAGAATACTTTATTACAGTCAACTTTATTACGACTATCCCCCC
TTTCTTGCGAAACTCCATTAGTAATAACTAATGAGCAGCATCGTTTTGTGGTCGCAGAACAATTGCGTCAAATAAACCAA
TTAAGCGATAACATTATTCTTGAACCATGTGGAAGAAATACTGCACCAGCTATAGCACTTTCAGCATTTACGGCTTTGAA
GCGTAATGAACAACAAGATCCTATACTTTTAGTTCTTGCAGCTGACCATGTCATAAACAAAACAGATGTTTTTTGTAACG
CTATCAAAAATTCCATTTCAATTGTTGAACAAGGAAATATCTTAACTTTTGGTATTATTCCTCATTATGCAGAGACCGGT
TATGGTTATATAAAAAAAGGGAATGTAATTAAAGAATCAGACAGTGGAGTTGGTAATAAATTTTACCAAGTAGAACAATT
CGTTGAAAAACCTAATCGCTCAAGGGCTGAAGAGTATATTTCCTCAGATAAATATCTTTGGAATAGTGGGATGTTTATGT
TTAAAGCATCAGTATATTTAGAGGAATTAAAAAAATACAGATCTGATATATACGATATATGTGAAAAAACAGTTTCTTCT
TCCTACCATGATCTTGACTTTATACGACTCTCGAAAGATGTTTTTCAAAATTGTCCATCGGAATCAATTGATTTTGCAGT
AATGGAAAAAACAAAGCGTTGTATAGTCTATCCCCTTGATATTGGTTGGAGCGATGTTGGATCTTGGCAATCGTTGTGGG
ACGTTAGTGACAAAACTCCTACAGGGGACGTTTGCAAAGGTGATATCCTGACCTATAATACAAAAAATAATTATATTCAC
TCGGAGTCAGCCTTAGTAGCCGCTGTAGGTGTAGAGGATATAGTTATTGTTCAGACGAAAGATGCAATTCTTGTTTCAAA
AAAATCTGAAGTTCAGGATGTTAAACAAATTGTCCAAATGCTCAAAAAGCAAGAACGTTCTGAATATCTATCACATCGCG
AGGAGTTTCGGCCTTGGGGGAAATTTGATGCTATAGAGCAGGGGGAACGATATAAAGTCAAAAAGATTGTCGTTAAACCA
GGGGAAGGGCTATCCTTACGTATGCATCATCATCGTTCAGAGCATTGGATTGTTCTTTCAGGCACAGCAAAAGTAACTTT
AAATAATAAAACCGTCTTAGTTACTGCTAATGAGTCCGTTTATATTCCTCTGGGCGCTACTTATAGCCTTGAAAATCCTG
GCGTAATTCCTCTTAATCTTATTGAGGTTAGTTCCGGAGATTATTTAGGTGAGGATGATATTGTTCGTCAAAAAGAACGT
TACAAAATAGATGACTAA

Upstream 100 bases:

>100_bases
CTGACGCCGGACGCGCTGCTCGCCAGTGACAATGTTCACGCTAACAGTCGAGCCTATTTTAATCATGCTCCAAGTGCTGT
TATTGGTTTAGATAAAAAGG

Downstream 100 bases:

>100_bases
TAGATGAAAAAATTAACCTGCTTTAAAGCCTACGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATCGCCTG
GCGTATTGGGCGTGCCTATG

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase 2; GMP 2; GMPP 2 [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSKMSDVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQSTLLRLSPLSCETPLVITNEQHRFVVAEQLRQINQ
LSDNIILEPCGRNTAPAIALSAFTALKRNEQQDPILLVLAADHVINKTDVFCNAIKNSISIVEQGNILTFGIIPHYAETG
YGYIKKGNVIKESDSGVGNKFYQVEQFVEKPNRSRAEEYISSDKYLWNSGMFMFKASVYLEELKKYRSDIYDICEKTVSS
SYHDLDFIRLSKDVFQNCPSESIDFAVMEKTKRCIVYPLDIGWSDVGSWQSLWDVSDKTPTGDVCKGDILTYNTKNNYIH
SESALVAAVGVEDIVIVQTKDAILVSKKSEVQDVKQIVQMLKKQERSEYLSHREEFRPWGKFDAIEQGERYKVKKIVVKP
GEGLSLRMHHHRSEHWIVLSGTAKVTLNNKTVLVTANESVYIPLGATYSLENPGVIPLNLIEVSSGDYLGEDDIVRQKER
YKIDD

Sequences:

>Translated_485_residues
MSKMSDVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQSTLLRLSPLSCETPLVITNEQHRFVVAEQLRQINQ
LSDNIILEPCGRNTAPAIALSAFTALKRNEQQDPILLVLAADHVINKTDVFCNAIKNSISIVEQGNILTFGIIPHYAETG
YGYIKKGNVIKESDSGVGNKFYQVEQFVEKPNRSRAEEYISSDKYLWNSGMFMFKASVYLEELKKYRSDIYDICEKTVSS
SYHDLDFIRLSKDVFQNCPSESIDFAVMEKTKRCIVYPLDIGWSDVGSWQSLWDVSDKTPTGDVCKGDILTYNTKNNYIH
SESALVAAVGVEDIVIVQTKDAILVSKKSEVQDVKQIVQMLKKQERSEYLSHREEFRPWGKFDAIEQGERYKVKKIVVKP
GEGLSLRMHHHRSEHWIVLSGTAKVTLNNKTVLVTANESVYIPLGATYSLENPGVIPLNLIEVSSGDYLGEDDIVRQKER
YKIDD
>Mature_484_residues
SKMSDVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQSTLLRLSPLSCETPLVITNEQHRFVVAEQLRQINQL
SDNIILEPCGRNTAPAIALSAFTALKRNEQQDPILLVLAADHVINKTDVFCNAIKNSISIVEQGNILTFGIIPHYAETGY
GYIKKGNVIKESDSGVGNKFYQVEQFVEKPNRSRAEEYISSDKYLWNSGMFMFKASVYLEELKKYRSDIYDICEKTVSSS
YHDLDFIRLSKDVFQNCPSESIDFAVMEKTKRCIVYPLDIGWSDVGSWQSLWDVSDKTPTGDVCKGDILTYNTKNNYIHS
ESALVAAVGVEDIVIVQTKDAILVSKKSEVQDVKQIVQMLKKQERSEYLSHREEFRPWGKFDAIEQGERYKVKKIVVKPG
EGLSLRMHHHRSEHWIVLSGTAKVTLNNKTVLVTANESVYIPLGATYSLENPGVIPLNLIEVSSGDYLGEDDIVRQKERY
KIDD

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=478, Percent_Identity=57.5313807531381, Blast_Score=588, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 54829; Mature: 54698

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKMSDVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQSTLLRLSPLSCETPL
CCCCCCCCEEEEEEECCCCCCCCCCCHHHCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCE
VITNEQHRFVVAEQLRQINQLSDNIILEPCGRNTAPAIALSAFTALKRNEQQDPILLVLA
EEECCCCEEHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
ADHVINKTDVFCNAIKNSISIVEQGNILTFGIIPHYAETGYGYIKKGNVIKESDSGVGNK
CCCCCCHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCCCCEEECCCEEECCCCCCCHH
FYQVEQFVEKPNRSRAEEYISSDKYLWNSGMFMFKASVYLEELKKYRSDIYDICEKTVSS
HHHHHHHHHCCCHHHHHHHHCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCC
SYHDLDFIRLSKDVFQNCPSESIDFAVMEKTKRCIVYPLDIGWSDVGSWQSLWDVSDKTP
CCCCCHHHEECHHHHHCCCCCCCCHHEECCCCCEEEEEECCCCCCCCCHHHHHCCCCCCC
TGDVCKGDILTYNTKNNYIHSESALVAAVGVEDIVIVQTKDAILVSKKSEVQDVKQIVQM
CCCCCCCCEEEEECCCCEEECCCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHH
LKKQERSEYLSHREEFRPWGKFDAIEQGERYKVKKIVVKPGEGLSLRMHHHRSEHWIVLS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCEEEEE
GTAKVTLNNKTVLVTANESVYIPLGATYSLENPGVIPLNLIEVSSGDYLGEDDIVRQKER
CEEEEEECCEEEEEEECCEEEEEECCEEECCCCCEEEEEEEEECCCCCCCCHHHHCCHHH
YKIDD
CCCCC
>Mature Secondary Structure 
SKMSDVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQSTLLRLSPLSCETPL
CCCCCCCEEEEEEECCCCCCCCCCCHHHCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCE
VITNEQHRFVVAEQLRQINQLSDNIILEPCGRNTAPAIALSAFTALKRNEQQDPILLVLA
EEECCCCEEHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
ADHVINKTDVFCNAIKNSISIVEQGNILTFGIIPHYAETGYGYIKKGNVIKESDSGVGNK
CCCCCCHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCCCCEEECCCEEECCCCCCCHH
FYQVEQFVEKPNRSRAEEYISSDKYLWNSGMFMFKASVYLEELKKYRSDIYDICEKTVSS
HHHHHHHHHCCCHHHHHHHHCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCC
SYHDLDFIRLSKDVFQNCPSESIDFAVMEKTKRCIVYPLDIGWSDVGSWQSLWDVSDKTP
CCCCCHHHEECHHHHHCCCCCCCCHHEECCCCCEEEEEECCCCCCCCCHHHHHCCCCCCC
TGDVCKGDILTYNTKNNYIHSESALVAAVGVEDIVIVQTKDAILVSKKSEVQDVKQIVQM
CCCCCCCCEEEEECCCCEEECCCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHH
LKKQERSEYLSHREEFRPWGKFDAIEQGERYKVKKIVVKPGEGLSLRMHHHRSEHWIVLS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCEEEEE
GTAKVTLNNKTVLVTANESVYIPLGATYSLENPGVIPLNLIEVSSGDYLGEDDIVRQKER
CEEEEEECCEEEEEEECCEEEEEECCEEECCCCCEEEEEEEEECCCCCCCCHHHHCCHHH
YKIDD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9673232; 11206551; 11258796 [H]