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The map label for this gene is rnfC [H]

Identifier: 218549355

GI number: 218549355

Start: 2073282

End: 2074622

Strand: Direct

Name: rnfC [H]

Synonym: EFER_2023

Alternate gene names: 218549355

Gene position: 2073282-2074622 (Clockwise)

Preceding gene: 218549354

Following gene: 218549356

Centisome position: 45.18

GC content: 50.93

Gene sequence:

>1341_bases
ATGAACAGAGTTATTGATTGCGATGGCGACACAATCCGCGAGCGTGTCCGTGCTGCGGGAGTCGTCGGTGCCGGCGGTGC
AGGTTTTCCAACATGGGTTAAATTACAGGCTCAGGTAGATACTTTTCTGGTCAACGCCGCTGAATGCGAACCCATGTTGA
AAGTTGATCAGCAATTGATGGCGCAGCAAGCAGAACGTCTGGCGCGTGGTGTCTTGTACGGAATGAAAGCCACTGGTGCG
AGTCACGGTATCATTGCCCTGAAAGAGAAATATCAACCTGCCATCAAAGCACTCTCTGAATTACTGCCAGCAGAAATCAG
GTTACATATTTTGCCTGATGTCTATCCGGCAGGAGATGAAGTATTAACCATCTGGCTGGCAACGGGCCGTCGGGTTCCTC
CCGCTGCGCTACCTGTCAGTGTGGGTGTAGTCGTTAACAATGTACAAACGGTACTCAATATCGCCAGAGCGGTTGAGCAA
GGTCTGGCAGTAACACGGCGAACGTTAACAGTTAACGGCGCGGTTGCCAGACCTGTAACACTGACGGTGCCGATTGGTAT
TTCGTTACGTGAAGTGCTGCAAATGGCGGGTGGCGCAACAATTGACACGCCAGGTTTTATCAATGGCGGTCCGATGATGG
GCAGCTTAATCACTTCACTGGATACCCCGGTCAGTAAAACAACAGGCGGATTGCTGGTACTTCCTGATTCTCACCCCCTT
ATCCAGCGCAGAAAGCAAAACGACAATGCCGTACTTTCCGTCGCACGTACTGTCTGCGAACAGTGCCGCTTGTGTACTGA
CCTGTGCCCACGACACCTTATAGGTCATGAGTTATCGCCGCATCTGCTGGTTCGTGCTGTTAACTTTCAATCTGTTGCAA
CACCACAGGTGCTACTAAGTGCCTTGACCTGTTCTGAATGTAACGTTTGTGAAAGTGTCGCCTGTCCGGTGGGTATTTCA
CCAATGCGAATTAATCGCATGATTAAACGTGAATTACGCGCACAAAATCTGCGTTATGAAGGGCCGTTAAATCCGCAGGA
TGCGATGGCGAAATATCGCCTCGTTCCCGTAAAGCGACTGATTGCTAAATTAGGTTTAGCCCCGTGGTATCACGATGCGC
CGTTCAGCAATGAACAGGCGGAAATTACCAGCACCACGCTTTTATTGCGCCAGCATATTGGCGCCAGTGCTATTCCCTGT
GTAAAACCTGGCGAACAAGTTACACGTGGACAGTGTGTGGCAGACATTCCTCAGGGAGCGCTGGGAGCGCCCATTCACGC
AAGCCATGACGGCATTATCAGCGAAGTTACAGAGCACGCCATTACGGTGGTAAGAGGTTAA

Upstream 100 bases:

>100_bases
TTCCGGTAATGGTTGAAGCGGCACTGGCTGACGCCACGCTTAAAACCAATCCCCGTGCCGCCAATGCCAGTGCCATCTGT
GAACTGCTTGAGGAGTTGCT

Downstream 100 bases:

>100_bases
ACATGTCGCAAGCAATAGGTATTTTGGAACTGACCAGTATCGCCAAAGGCATGGAAACAGGCGATGCCATGTTAAAGAGT
TCCAGTGTGGATTTACTTGT

Product: propanediol utilization protein: ferredoxin pduS

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MNRVIDCDGDTIRERVRAAGVVGAGGAGFPTWVKLQAQVDTFLVNAAECEPMLKVDQQLMAQQAERLARGVLYGMKATGA
SHGIIALKEKYQPAIKALSELLPAEIRLHILPDVYPAGDEVLTIWLATGRRVPPAALPVSVGVVVNNVQTVLNIARAVEQ
GLAVTRRTLTVNGAVARPVTLTVPIGISLREVLQMAGGATIDTPGFINGGPMMGSLITSLDTPVSKTTGGLLVLPDSHPL
IQRRKQNDNAVLSVARTVCEQCRLCTDLCPRHLIGHELSPHLLVRAVNFQSVATPQVLLSALTCSECNVCESVACPVGIS
PMRINRMIKRELRAQNLRYEGPLNPQDAMAKYRLVPVKRLIAKLGLAPWYHDAPFSNEQAEITSTTLLLRQHIGASAIPC
VKPGEQVTRGQCVADIPQGALGAPIHASHDGIISEVTEHAITVVRG

Sequences:

>Translated_446_residues
MNRVIDCDGDTIRERVRAAGVVGAGGAGFPTWVKLQAQVDTFLVNAAECEPMLKVDQQLMAQQAERLARGVLYGMKATGA
SHGIIALKEKYQPAIKALSELLPAEIRLHILPDVYPAGDEVLTIWLATGRRVPPAALPVSVGVVVNNVQTVLNIARAVEQ
GLAVTRRTLTVNGAVARPVTLTVPIGISLREVLQMAGGATIDTPGFINGGPMMGSLITSLDTPVSKTTGGLLVLPDSHPL
IQRRKQNDNAVLSVARTVCEQCRLCTDLCPRHLIGHELSPHLLVRAVNFQSVATPQVLLSALTCSECNVCESVACPVGIS
PMRINRMIKRELRAQNLRYEGPLNPQDAMAKYRLVPVKRLIAKLGLAPWYHDAPFSNEQAEITSTTLLLRQHIGASAIPC
VKPGEQVTRGQCVADIPQGALGAPIHASHDGIISEVTEHAITVVRG
>Mature_446_residues
MNRVIDCDGDTIRERVRAAGVVGAGGAGFPTWVKLQAQVDTFLVNAAECEPMLKVDQQLMAQQAERLARGVLYGMKATGA
SHGIIALKEKYQPAIKALSELLPAEIRLHILPDVYPAGDEVLTIWLATGRRVPPAALPVSVGVVVNNVQTVLNIARAVEQ
GLAVTRRTLTVNGAVARPVTLTVPIGISLREVLQMAGGATIDTPGFINGGPMMGSLITSLDTPVSKTTGGLLVLPDSHPL
IQRRKQNDNAVLSVARTVCEQCRLCTDLCPRHLIGHELSPHLLVRAVNFQSVATPQVLLSALTCSECNVCESVACPVGIS
PMRINRMIKRELRAQNLRYEGPLNPQDAMAKYRLVPVKRLIAKLGLAPWYHDAPFSNEQAEITSTTLLLRQHIGASAIPC
VKPGEQVTRGQCVADIPQGALGAPIHASHDGIISEVTEHAITVVRG

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=327, Percent_Identity=31.4984709480122, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788620, Length=240, Percent_Identity=28.3333333333333, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 47769; Mature: 47769

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVIDCDGDTIRERVRAAGVVGAGGAGFPTWVKLQAQVDTFLVNAAECEPMLKVDQQLM
CCCEEECCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHEEEHHHCCHHHHHHHHHH
AQQAERLARGVLYGMKATGASHGIIALKEKYQPAIKALSELLPAEIRLHILPDVYPAGDE
HHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCC
VLTIWLATGRRVPPAALPVSVGVVVNNVQTVLNIARAVEQGLAVTRRTLTVNGAVARPVT
EEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEECCEE
LTVPIGISLREVLQMAGGATIDTPGFINGGPMMGSLITSLDTPVSKTTGGLLVLPDSHPL
EEEECCCCHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCHH
IQRRKQNDNAVLSVARTVCEQCRLCTDLCPRHLIGHELSPHLLVRAVNFQSVATPQVLLS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEECCCHHCCHHHHHH
ALTCSECNVCESVACPVGISPMRINRMIKRELRAQNLRYEGPLNPQDAMAKYRLVPVKRL
HHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IAKLGLAPWYHDAPFSNEQAEITSTTLLLRQHIGASAIPCVKPGEQVTRGQCVADIPQGA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEECCCCCC
LGAPIHASHDGIISEVTEHAITVVRG
CCCCCCCCCCCHHHHHHHHHHEEECC
>Mature Secondary Structure
MNRVIDCDGDTIRERVRAAGVVGAGGAGFPTWVKLQAQVDTFLVNAAECEPMLKVDQQLM
CCCEEECCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHEEEHHHCCHHHHHHHHHH
AQQAERLARGVLYGMKATGASHGIIALKEKYQPAIKALSELLPAEIRLHILPDVYPAGDE
HHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCC
VLTIWLATGRRVPPAALPVSVGVVVNNVQTVLNIARAVEQGLAVTRRTLTVNGAVARPVT
EEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEECCEE
LTVPIGISLREVLQMAGGATIDTPGFINGGPMMGSLITSLDTPVSKTTGGLLVLPDSHPL
EEEECCCCHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCHH
IQRRKQNDNAVLSVARTVCEQCRLCTDLCPRHLIGHELSPHLLVRAVNFQSVATPQVLLS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEECCCHHCCHHHHHH
ALTCSECNVCESVACPVGISPMRINRMIKRELRAQNLRYEGPLNPQDAMAKYRLVPVKRL
HHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IAKLGLAPWYHDAPFSNEQAEITSTTLLLRQHIGASAIPCVKPGEQVTRGQCVADIPQGA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEECCCCCC
LGAPIHASHDGIISEVTEHAITVVRG
CCCCCCCCCCCHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]