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The map label for this gene is adh1 [H]

Identifier: 218549354

GI number: 218549354

Start: 2072173

End: 2073285

Strand: Direct

Name: adh1 [H]

Synonym: EFER_2022

Alternate gene names: 218549354

Gene position: 2072173-2073285 (Clockwise)

Preceding gene: 218549353

Following gene: 218549355

Centisome position: 45.16

GC content: 49.15

Gene sequence:

>1113_bases
ATGAAAACCTTTTCCCTGCAAACGCGCCTGTACAGTGGCATCGGTAGTCTGAAAGTACTGAACCGCTTTACTCACAGGCA
TATCTGGATAATTTGCGATGGTTTTCTGGCACATTCTCCGCTGCTGGAAACCTTGCGCGGCGCGCTGCCTGCGGATAACA
AGATCAGCATTTTTACTGATATCACTCCCGATCCAACTATTGAGACCGTGGTTAAAGGGATTGCGCAAATGCAGTCCCTG
CGCCCGGACGTCGTGATTGGTTTTGGTGGCGGCTCAGCACTGGATGCGGCAAAGGCCATTGTCTGGTTTAGCCGTCAATT
CGGTATTGAAATTGAAACCTGCGTGGCTATCCCCACCACCAGCGGTACAGGCTCAGAAGTGACCAGCGCTTGCGTGATTA
GTGATACACAAAAGGGCATTAAATATCCGTTGTTTGATAAAGAATTGTATCCGGATATGGCTATTCTCGACCCTTCCTTT
GTGGTGAGTGTCCCCGCCAATATTACTGCAAACACCGGAATGGATGTTCTTACCCATGCGTTGGAAGCTTATGTTTCTCC
TCGTGCCAGTGATTTTACCGATGCACTGGCAGAAAAAGCTGCGCAAATTGTCTTTCAATATTTACCCGTCGCAGTAACAA
AAGGTGATTGCCTGGCGACGAGAGGCAAAATGCATAACGCCGCTACCCTTGCAGGTATGGCTTTTAGTCAGGCTGGACTG
GGATTAAATCATGCCATTGCGCATCAACTCGGTGGACAGTTTCATTTGCCACACGGACTTGCTAATGCCCTGTTATTAAA
TGCTGTTATTCGTTTTAACGCCGGGGAGTTGCGTGCTTTAAAGCGCTATGCACGACTCGCCAAAGCCTGTCATCTCTGCC
CGGACAACGCCAGTGACAACGCTGCGCTCAGTGCTCTTATCCAGCGAATTGAATCACTAAAAAAAGCCTGTGCAATTCCC
TCCATTCACATTGCACTTAAAGAAAACACAATGCACTGGTCACAACGTATTCCGGTAATGGTTGAAGCGGCACTGGCTGA
CGCCACGCTTAAAACCAATCCCCGTGCCGCCAATGCCAGTGCCATCTGTGAACTGCTTGAGGAGTTGCTATGA

Upstream 100 bases:

>100_bases
GCTACCCCCACGGGTGAAGGTACTACCTCGGCCAAAACGTTTGCCCGTTCCCGTCGTTGCGTGTTGACCAGCGGTTTTTC
GATCCGTTAATGAGGGTGCA

Downstream 100 bases:

>100_bases
ACAGAGTTATTGATTGCGATGGCGACACAATCCGCGAGCGTGTCCGTGCTGCGGGAGTCGTCGGTGCCGGCGGTGCAGGT
TTTCCAACATGGGTTAAATT

Product: Propanediol utilization propanol dehydrogenase pduQ

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKTFSLQTRLYSGIGSLKVLNRFTHRHIWIICDGFLAHSPLLETLRGALPADNKISIFTDITPDPTIETVVKGIAQMQSL
RPDVVIGFGGGSALDAAKAIVWFSRQFGIEIETCVAIPTTSGTGSEVTSACVISDTQKGIKYPLFDKELYPDMAILDPSF
VVSVPANITANTGMDVLTHALEAYVSPRASDFTDALAEKAAQIVFQYLPVAVTKGDCLATRGKMHNAATLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLNAVIRFNAGELRALKRYARLAKACHLCPDNASDNAALSALIQRIESLKKACAIP
SIHIALKENTMHWSQRIPVMVEAALADATLKTNPRAANASAICELLEELL

Sequences:

>Translated_370_residues
MKTFSLQTRLYSGIGSLKVLNRFTHRHIWIICDGFLAHSPLLETLRGALPADNKISIFTDITPDPTIETVVKGIAQMQSL
RPDVVIGFGGGSALDAAKAIVWFSRQFGIEIETCVAIPTTSGTGSEVTSACVISDTQKGIKYPLFDKELYPDMAILDPSF
VVSVPANITANTGMDVLTHALEAYVSPRASDFTDALAEKAAQIVFQYLPVAVTKGDCLATRGKMHNAATLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLNAVIRFNAGELRALKRYARLAKACHLCPDNASDNAALSALIQRIESLKKACAIP
SIHIALKENTMHWSQRIPVMVEAALADATLKTNPRAANASAICELLEELL
>Mature_370_residues
MKTFSLQTRLYSGIGSLKVLNRFTHRHIWIICDGFLAHSPLLETLRGALPADNKISIFTDITPDPTIETVVKGIAQMQSL
RPDVVIGFGGGSALDAAKAIVWFSRQFGIEIETCVAIPTTSGTGSEVTSACVISDTQKGIKYPLFDKELYPDMAILDPSF
VVSVPANITANTGMDVLTHALEAYVSPRASDFTDALAEKAAQIVFQYLPVAVTKGDCLATRGKMHNAATLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLNAVIRFNAGELRALKRYARLAKACHLCPDNASDNAALSALIQRIESLKKACAIP
SIHIALKENTMHWSQRIPVMVEAALADATLKTNPRAANASAICELLEELL

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=300, Percent_Identity=26.3333333333333, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787493, Length=385, Percent_Identity=35.0649350649351, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI87082107, Length=382, Percent_Identity=37.17277486911, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI48994951, Length=352, Percent_Identity=36.0795454545455, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789163, Length=328, Percent_Identity=34.7560975609756, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789386, Length=344, Percent_Identity=26.7441860465116, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=369, Percent_Identity=25.4742547425474, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321181, Length=316, Percent_Identity=36.0759493670886, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24657991, Length=416, Percent_Identity=24.2788461538462, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 39679; Mature: 39679

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFSLQTRLYSGIGSLKVLNRFTHRHIWIICDGFLAHSPLLETLRGALPADNKISIFTD
CCCCHHHHHHHHCCCHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEEC
ITPDPTIETVVKGIAQMQSLRPDVVIGFGGGSALDAAKAIVWFSRQFGIEIETCVAIPTT
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECC
SGTGSEVTSACVISDTQKGIKYPLFDKELYPDMAILDPSFVVSVPANITANTGMDVLTHA
CCCCHHHHHHHEECCCCCCCCCCCCCHHHCCCCEEECCCEEEEECCCCCCCCCHHHHHHH
LEAYVSPRASDFTDALAEKAAQIVFQYLPVAVTKGDCLATRGKMHNAATLAGMAFSQAGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCCCCCCHHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLNAVIRFNAGELRALKRYARLAKACHLCPDNASDN
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
AALSALIQRIESLKKACAIPSIHIALKENTMHWSQRIPVMVEAALADATLKTNPRAANAS
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCEEEHHHHCCCCCCCCCCCCHH
AICELLEELL
HHHHHHHHHC
>Mature Secondary Structure
MKTFSLQTRLYSGIGSLKVLNRFTHRHIWIICDGFLAHSPLLETLRGALPADNKISIFTD
CCCCHHHHHHHHCCCHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEEC
ITPDPTIETVVKGIAQMQSLRPDVVIGFGGGSALDAAKAIVWFSRQFGIEIETCVAIPTT
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECC
SGTGSEVTSACVISDTQKGIKYPLFDKELYPDMAILDPSFVVSVPANITANTGMDVLTHA
CCCCHHHHHHHEECCCCCCCCCCCCCHHHCCCCEEECCCEEEEECCCCCCCCCHHHHHHH
LEAYVSPRASDFTDALAEKAAQIVFQYLPVAVTKGDCLATRGKMHNAATLAGMAFSQAGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCCCCCCHHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLNAVIRFNAGELRALKRYARLAKACHLCPDNASDN
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
AALSALIQRIESLKKACAIPSIHIALKENTMHWSQRIPVMVEAALADATLKTNPRAANAS
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCEEEHHHHCCCCCCCCCCCCHH
AICELLEELL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]