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The map label for this gene is tnpR [H]

Identifier: 218548994

GI number: 218548994

Start: 1689743

End: 1690336

Strand: Direct

Name: tnpR [H]

Synonym: EFER_1642

Alternate gene names: 218548994

Gene position: 1689743-1690336 (Clockwise)

Preceding gene: 218548992

Following gene: 218548995

Centisome position: 36.82

GC content: 51.35

Gene sequence:

>594_bases
ATGAGTTGTCCGATATTCGATTTAAGGTACATTTTTATGCGACTTTTTGGTTACGCTCGGGTCTCAACCAGTCAGCAGTC
TCTTGATCTTCAGGTCAGAGCACTCAAAGACGCAGGTGTGAAAGCAAACCGTATATTTACCGATAAGGCATCCGGCAGTT
CAACAGACCGGGAAGGGCTGGATTTGCTGAGGATGAAGGTGGAGGAAGGTGATGTCATTCTGGTTAAGAAGCTCGACCGT
CTTGGCCGCGACACTGCCGATATGATCCAACTGATAAAGGAATTTGACGCTCAGGGCGTGGCAGTCCGGTTCATTGATGA
CGGGATCAGTACCGACGGTGATATGGGGCAAATGGTGGTCACCATCCTGTCGGCTGTGGCACAGGCTGAACGCCGGAGGA
TCCTAGAACGCACGAATGAGGGCCGACAGGAAGCAAAGCTGAAAGGAATCAAATTTGGCCGCAGGCGTACCGTGGACAGG
AACGTCGTGCTGACGCTTCATCAGAAGGGCACTGGTGCAACGGAAATTGCTCATCAGCTCAGTATTGCCCGCTCCACGGT
TTATAAAATTCTTGAAGACGAAAGGGCCTCGTGA

Upstream 100 bases:

>100_bases
AGTAACATTTTTCCAACCGTACGAAATGTTATAAATTATCGGACATCGTAAAACTGTTACATTAATATGTCTATTAAATC
GTAAATTTGTAATAATAGAC

Downstream 100 bases:

>100_bases
TACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATT

Product: Resolvase transposon Tn3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSCPIFDLRYIFMRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDREGLDLLRMKVEEGDVILVKKLDR
LGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDR
NVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS

Sequences:

>Translated_197_residues
MSCPIFDLRYIFMRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDREGLDLLRMKVEEGDVILVKKLDR
LGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDR
NVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS
>Mature_196_residues
SCPIFDLRYIFMRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDREGLDLLRMKVEEGDVILVKKLDRL
GRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRN
VVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=37.0786516853933, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787638, Length=184, Percent_Identity=31.5217391304348, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1787827, Length=184, Percent_Identity=30.4347826086957, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22091; Mature: 21960

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCPIFDLRYIFMRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDREGL
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEHHHCCCCCCEEEECCCCCCCCCHHHH
DLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVV
HHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHH
TILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHH
SIARSTVYKILEDERAS
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SCPIFDLRYIFMRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDREGL
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEHHHCCCCCCEEEECCCCCCCCCHHHH
DLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVV
HHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHH
TILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHH
SIARSTVYKILEDERAS
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 391406 [H]