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The map label for this gene is ydaO [H]

Identifier: 218548977

GI number: 218548977

Start: 1672827

End: 1673762

Strand: Direct

Name: ydaO [H]

Synonym: EFER_1625

Alternate gene names: 218548977

Gene position: 1672827-1673762 (Clockwise)

Preceding gene: 218548976

Following gene: 218548982

Centisome position: 36.46

GC content: 46.58

Gene sequence:

>936_bases
ATGTCGCAAAATCAAGAATTTAGTAAGAAAGAAATCTACAACCTGAACAAATTGCAGAAACGTCTGCGCCGTAACGTGGG
CGAAGCGATTGCTGACTTCAATATGATTGAGGATGGTGATCGCATTATGGTTTGCCTTTCCGGTGGCAAAGACAGTTACA
CCATGCTGGAGATCCTGCGCAATTTACAACAAAGCGCGCCGATCAGTTTTTCGCTGGTGGCGGTGAATCTTGATCAAAAA
CAACCAGGATTCCCTGAACATATTCTGCCGGAATATCTGGAAAAGCTTGGTGTTGAATACAAGATTGTCGAAGAAAATAC
CTACGGTATCGTTAAAGAGAAGATCCCGGAAGGTAAAACGACATGTTCTCTGTGTTCACGTCTGCGCCGTGGTATTTTGT
ACCGTACCGCTACCGAACTCGGTGCAACCAAAATCGCGCTGGGCCACCATCGCGACGATATCCTGCAAACGCTGTTTTTG
AACATGTTCTATGGCGGTAAGATGAAAGGTATGCCGCCAAAACTAATGAGCGATGATGGTAAACATATTGTGATTCGCCC
GCTGGCCTATTGTCGTGAGAAAGATATCCAGCGTTTTGCGGATGCCAAAGCATTCCCCATTATTCCATGCAACCTGTGTG
GATCGCAGCCAAACCTGCAACGCCAGGTTATCGCAGATATGCTGCGCGACTGGGACAAACGCTATCCGGGGCGTATCGAG
ACCATGTTCAGCGCAATGCAAAACGTGGTGCCTTCACATTTGTGTGATACCAATTTGTTTGATTTCAAAGGCATTAAACA
CGGTTTTGAAGTGATTAATGGCGGCGATTTGGCATTTGACCGCGAAGAGCTCCCACTGCAACCTGCGGGATGGCAACCAG
AAGATGATGAAAATCAACTTGATGAGTTGCGATTGAACGTGGTGGAAGTCAAATAA

Upstream 100 bases:

>100_bases
TACCATACACGCCACAGTTTTATATCAGACTTCTTTTGCCGGATTTTCCGGCGTGATGCATACTCTTCTGATGCCACATA
ATGAATTGAGCCACTATTAA

Downstream 100 bases:

>100_bases
CTGACCTGTCATTCATTGCCCGATGCGCAAGATTATCGGGCATGAATACCAGTATTAATCCAGTTATGTGTATGCCGGAG
AAAATGCTTCTGTATTGCTT

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSQNQEFSKKEIYNLNKLQKRLRRNVGEAIADFNMIEDGDRIMVCLSGGKDSYTMLEILRNLQQSAPISFSLVAVNLDQK
QPGFPEHILPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGIKHGFEVINGGDLAFDREELPLQPAGWQPEDDENQLDELRLNVVEVK

Sequences:

>Translated_311_residues
MSQNQEFSKKEIYNLNKLQKRLRRNVGEAIADFNMIEDGDRIMVCLSGGKDSYTMLEILRNLQQSAPISFSLVAVNLDQK
QPGFPEHILPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGIKHGFEVINGGDLAFDREELPLQPAGWQPEDDENQLDELRLNVVEVK
>Mature_310_residues
SQNQEFSKKEIYNLNKLQKRLRRNVGEAIADFNMIEDGDRIMVCLSGGKDSYTMLEILRNLQQSAPISFSLVAVNLDQKQ
PGFPEHILPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLN
MFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIET
MFSAMQNVVPSHLCDTNLFDFKGIKHGFEVINGGDLAFDREELPLQPAGWQPEDDENQLDELRLNVVEVK

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=311, Percent_Identity=95.1768488745981, Blast_Score=622, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI193211090, Length=260, Percent_Identity=31.5384615384615, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17540090, Length=235, Percent_Identity=27.2340425531915, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=26.9230769230769, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35539; Mature: 35408

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNQEFSKKEIYNLNKLQKRLRRNVGEAIADFNMIEDGDRIMVCLSGGKDSYTMLEILR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPISFSLVAVNLDQKQPGFPEHILPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
HHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHHHHHHHHHHCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGIKHGFEVINGGDLAFDREELPLQPAGWQPEDDENQL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCEECHHHCCCCCCCCCCCCCHHHH
DELRLNVVEVK
HHHHHEEEECC
>Mature Secondary Structure 
SQNQEFSKKEIYNLNKLQKRLRRNVGEAIADFNMIEDGDRIMVCLSGGKDSYTMLEILR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPISFSLVAVNLDQKQPGFPEHILPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
HHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHHHHHHHHHHCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGIKHGFEVINGGDLAFDREELPLQPAGWQPEDDENQL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCEECHHHCCCCCCCCCCCCCHHHH
DELRLNVVEVK
HHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA