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The map label for this gene is rob [H]

Identifier: 218548923

GI number: 218548923

Start: 1601925

End: 1602725

Strand: Direct

Name: rob [H]

Synonym: EFER_1569

Alternate gene names: 218548923

Gene position: 1601925-1602725 (Clockwise)

Preceding gene: 218548921

Following gene: 218548926

Centisome position: 34.91

GC content: 36.2

Gene sequence:

>801_bases
ATGATAAACATCTTCGCCTGGATAAACAAAAATATAGAATCCCCTATTAACGTTGAAGATCTGGTAAAAATTTCAGGTTA
CAGCAGAAGACATTTACATACACTGTTTATGCAATATACAGGACACAGTGTCGCGGAATACGTACGTTTGAAACGCCTGT
CCTATACAGCTCATCTGCTCAAACTCACCAATCTGTCCGTGCTGGATATATCATTACGTTATCACTACGACTCGCCACAG
AGTTTTAGTCGGGTTTTTAAGCGTTATTATCAAATGACACCCCTCGCCTATCGTCGAAGCCAGCAATGGGGTAAAAAAAT
TCATTTTGTTGATCCATCAGAAAGAACAGATGTTGTCTATGATTTTGTTTATTATGAAGGTGATACTTTTTCAGGGAGCC
TCCATCAGTTTCATCTCGATATGTCATCGCGTTTAGATTTCTGGCAAAAAGCAGCTATGCAAATAAATGATATGAAATTT
CGCTCACTTCAACATACTTCGCAAATTAGAAGTAGTGTAAGCTACTACCCTTCCCTTCATAGTAAAAGCTCCATGGTTGT
TGATTATGTATATGATGATGGAGCAAATAGTAAAACAGATATTGCAATAGCAAGTGGTGATTATATTAAAATTTCTCGAC
GAGGTATTTTGAATGACATGGTCGGATTGCCGAATGTTTTATATAGAAACATTCTTTATGCACATAATCTCATCAGAGGT
AATGGCCGTGATTTCGAAATTATTGAAAGAGACAAAGGTCATACAATTAATTTCTCTTACTATATTCCTGTAAAAATGTA
A

Upstream 100 bases:

>100_bases
CACAAAAGTTAACAAAACACTACAGAACAAAGCATAACTCTGCGTTCCGTTGAATTGAATTGCGTTAGACTAAAATACCT
GCATATATCACTAATAAGTC

Downstream 100 bases:

>100_bases
TCTACTTTGGATGTTACATTTTGAATAACGGCTACGTCGCCCCGCAGCAAACGGATCTGAAGCCGAACACGCCCGTATAT
GCAGCACATGCAACTTGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MINIFAWINKNIESPINVEDLVKISGYSRRHLHTLFMQYTGHSVAEYVRLKRLSYTAHLLKLTNLSVLDISLRYHYDSPQ
SFSRVFKRYYQMTPLAYRRSQQWGKKIHFVDPSERTDVVYDFVYYEGDTFSGSLHQFHLDMSSRLDFWQKAAMQINDMKF
RSLQHTSQIRSSVSYYPSLHSKSSMVVDYVYDDGANSKTDIAIASGDYIKISRRGILNDMVGLPNVLYRNILYAHNLIRG
NGRDFEIIERDKGHTINFSYYIPVKM

Sequences:

>Translated_266_residues
MINIFAWINKNIESPINVEDLVKISGYSRRHLHTLFMQYTGHSVAEYVRLKRLSYTAHLLKLTNLSVLDISLRYHYDSPQ
SFSRVFKRYYQMTPLAYRRSQQWGKKIHFVDPSERTDVVYDFVYYEGDTFSGSLHQFHLDMSSRLDFWQKAAMQINDMKF
RSLQHTSQIRSSVSYYPSLHSKSSMVVDYVYDDGANSKTDIAIASGDYIKISRRGILNDMVGLPNVLYRNILYAHNLIRG
NGRDFEIIERDKGHTINFSYYIPVKM
>Mature_266_residues
MINIFAWINKNIESPINVEDLVKISGYSRRHLHTLFMQYTGHSVAEYVRLKRLSYTAHLLKLTNLSVLDISLRYHYDSPQ
SFSRVFKRYYQMTPLAYRRSQQWGKKIHFVDPSERTDVVYDFVYYEGDTFSGSLHQFHLDMSSRLDFWQKAAMQINDMKF
RSLQHTSQIRSSVSYYPSLHSKSSMVVDYVYDDGANSKTDIAIASGDYIKISRRGILNDMVGLPNVLYRNILYAHNLIRG
NGRDFEIIERDKGHTINFSYYIPVKM

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=101, Percent_Identity=38.6138613861386, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081928, Length=110, Percent_Identity=36.3636363636364, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790497, Length=96, Percent_Identity=37.5, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 31261; Mature: 31261

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIFAWINKNIESPINVEDLVKISGYSRRHLHTLFMQYTGHSVAEYVRLKRLSYTAHLL
CEEEEEEECCCCCCCCCHHHHHEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KLTNLSVLDISLRYHYDSPQSFSRVFKRYYQMTPLAYRRSQQWGKKIHFVDPSERTDVVY
HHCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCCCCCEEE
DFVYYEGDTFSGSLHQFHLDMSSRLDFWQKAAMQINDMKFRSLQHTSQIRSSVSYYPSLH
EEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
SKSSMVVDYVYDDGANSKTDIAIASGDYIKISRRGILNDMVGLPNVLYRNILYAHNLIRG
CCCCEEEEEEEECCCCCCCEEEEECCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCC
NGRDFEIIERDKGHTINFSYYIPVKM
CCCCEEEEECCCCCEEEEEEEEEEEC
>Mature Secondary Structure
MINIFAWINKNIESPINVEDLVKISGYSRRHLHTLFMQYTGHSVAEYVRLKRLSYTAHLL
CEEEEEEECCCCCCCCCHHHHHEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KLTNLSVLDISLRYHYDSPQSFSRVFKRYYQMTPLAYRRSQQWGKKIHFVDPSERTDVVY
HHCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCCCCCEEE
DFVYYEGDTFSGSLHQFHLDMSSRLDFWQKAAMQINDMKFRSLQHTSQIRSSVSYYPSLH
EEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
SKSSMVVDYVYDDGANSKTDIAIASGDYIKISRRGILNDMVGLPNVLYRNILYAHNLIRG
CCCCEEEEEEEECCCCCCCEEEEECCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCC
NGRDFEIIERDKGHTINFSYYIPVKM
CCCCEEEEECCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]