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The map label for this gene is pncA [H]

Identifier: 218548662

GI number: 218548662

Start: 1334620

End: 1335297

Strand: Reverse

Name: pncA [H]

Synonym: EFER_1296

Alternate gene names: 218548662

Gene position: 1335297-1334620 (Counterclockwise)

Preceding gene: 218548663

Following gene: 218548659

Centisome position: 29.1

GC content: 48.23

Gene sequence:

>678_bases
GTGGCGAGCTTACGCCAGATGACTAAGGAGATTTTGATGACGCATCGCGCTCTGCTACTGGTAGATTTGCAAAATGATTT
TTGTGCCGGAGGTGCGCTTGCTGTACCGGAAGGTGACAACACCGTTGATATTGCTAACCAGCTGATTAACTGGTGTCAAT
CACGGGATATACCGGTTATCGCCAGCCAGGACTGGCATCCGGCTAATCACGGCAGCTTTGCCAGTCAGCATCAGGTTGCA
GTCTACAGCCAGGGAATGCTTGACGGTTTACCACAAACGTTCTGGCCTGATCACTGTGTACAGAATAGCGAAGGTGCTGC
ATTGCATCCTTTGCTGAAACAAAAAGCTATCAATCGCATTTTTCACAAAGGCGAGAATCCGTTAGTTGATAGCTATAGCG
CCTTTTTTGATAACGGGCGGCGGCAAAAAACCACGCTGGATGAGTGGCTACGTGAACATAATATTGGCGAATTGATAATT
ATGGGTCTTGCCACTGACTACTGCGTTAAATTTACTGTTCTGGATGCCCTGCAACTGGGTTACCGTGTCAACGTCATCAC
TGATGGTTGCCGGGGAGTGAATATTAACCCACAAGATAGCGCTCAGGCATTTATGGAAATGTCTGTTGCTGGTGCCACGT
TATATACGCTGGCAGACTGGCAAGAGACGCAGGAGTAA

Upstream 100 bases:

>100_bases
GCTGATATGACAGTAGAAGCTACGCTGACGAAGTTGCATTATTTGCTCAGCCAGGGGCTGGATACCCAGACTATTCGTCA
AGTCATGACTGAAAACTTGC

Downstream 100 bases:

>100_bases
AGTTTAACCGGGTGACAAATTTCAGACACGTCACCCGTTCTGTTTTTGTCTGTCACCCAACTCACTGCACTTTCAGCGTC
GCAAACGACTTCGGAGCGAT

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MASLRQMTKEILMTHRALLLVDLQNDFCAGGALAVPEGDNTVDIANQLINWCQSRDIPVIASQDWHPANHGSFASQHQVA
VYSQGMLDGLPQTFWPDHCVQNSEGAALHPLLKQKAINRIFHKGENPLVDSYSAFFDNGRRQKTTLDEWLREHNIGELII
MGLATDYCVKFTVLDALQLGYRVNVITDGCRGVNINPQDSAQAFMEMSVAGATLYTLADWQETQE

Sequences:

>Translated_225_residues
MASLRQMTKEILMTHRALLLVDLQNDFCAGGALAVPEGDNTVDIANQLINWCQSRDIPVIASQDWHPANHGSFASQHQVA
VYSQGMLDGLPQTFWPDHCVQNSEGAALHPLLKQKAINRIFHKGENPLVDSYSAFFDNGRRQKTTLDEWLREHNIGELII
MGLATDYCVKFTVLDALQLGYRVNVITDGCRGVNINPQDSAQAFMEMSVAGATLYTLADWQETQE
>Mature_224_residues
ASLRQMTKEILMTHRALLLVDLQNDFCAGGALAVPEGDNTVDIANQLINWCQSRDIPVIASQDWHPANHGSFASQHQVAV
YSQGMLDGLPQTFWPDHCVQNSEGAALHPLLKQKAINRIFHKGENPLVDSYSAFFDNGRRQKTTLDEWLREHNIGELIIM
GLATDYCVKFTVLDALQLGYRVNVITDGCRGVNINPQDSAQAFMEMSVAGATLYTLADWQETQE

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=212, Percent_Identity=85.377358490566, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI193206735, Length=218, Percent_Identity=36.2385321100917, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17543130, Length=218, Percent_Identity=36.2385321100917, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71999339, Length=228, Percent_Identity=29.8245614035088, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321401, Length=196, Percent_Identity=31.6326530612245, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648247, Length=213, Percent_Identity=35.6807511737089, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 25088; Mature: 24956

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLRQMTKEILMTHRALLLVDLQNDFCAGGALAVPEGDNTVDIANQLINWCQSRDIPVI
CCHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEE
ASQDWHPANHGSFASQHQVAVYSQGMLDGLPQTFWPDHCVQNSEGAALHPLLKQKAINRI
ECCCCCCCCCCCCCCCCHHHHHHCHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHH
FHKGENPLVDSYSAFFDNGRRQKTTLDEWLREHNIGELIIMGLATDYCVKFTVLDALQLG
HHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHC
YRVNVITDGCRGVNINPQDSAQAFMEMSVAGATLYTLADWQETQE
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCHHHCCC
>Mature Secondary Structure 
ASLRQMTKEILMTHRALLLVDLQNDFCAGGALAVPEGDNTVDIANQLINWCQSRDIPVI
CHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEE
ASQDWHPANHGSFASQHQVAVYSQGMLDGLPQTFWPDHCVQNSEGAALHPLLKQKAINRI
ECCCCCCCCCCCCCCCCHHHHHHCHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHH
FHKGENPLVDSYSAFFDNGRRQKTTLDEWLREHNIGELIIMGLATDYCVKFTVLDALQLG
HHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHC
YRVNVITDGCRGVNINPQDSAQAFMEMSVAGATLYTLADWQETQE
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]