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The map label for this gene is ansA [H]

Identifier: 218548663

GI number: 218548663

Start: 1335272

End: 1336288

Strand: Reverse

Name: ansA [H]

Synonym: EFER_1297

Alternate gene names: 218548663

Gene position: 1336288-1335272 (Counterclockwise)

Preceding gene: 218548664

Following gene: 218548662

Centisome position: 29.12

GC content: 48.48

Gene sequence:

>1017_bases
ATGCACAAGAAATCAATTTATGTGGCTTATACCGGCGGTACAATCGGTATGCAACGCTCAGAACAGGGTTATATTCCGGT
TTCAGGGCATCTTCAACGTCAGTTAGCACTGATGCCGGAATTCCATCGCCCGGAGATGCCGGATTTTACCATCCATGAAT
ATGCGCCGCTGATGGATTCTTCCGATATGACGCCTCAGGACTGGCAACATATCGCCGAAGATATTCAGGCCCATTATGAT
GAGTACGACGGATTTGTCATTTTGCATGGTACAGATACCATGGCTTATACCGCTTCCGCACTCTCGTTTATGCTGGAGAA
TTTGAGCAAACCTGTAATAGTGACAGGGTCACAAATACCTTTAGCCGAGCTGCGATCTGACGGGCAGATCAATTTGCTCA
ATGCGCTGTATGTTGCAGCTAATTACCCGATTAACGAAGTCACACTATTTTTTAATAACCGTCTGTATCGGGGTAATCGT
ACGACCAAAGCCCATGCCGATGGTTTTGATGCCTTTGCCTCACCTAATCTTCCCCCATTGCTTGAAGCCGGGATTCACAT
TCGCCGTCTGTCAACACCAGCAGCACCGCAAGGTCAGGGAGAGCTGATCGTTCACCCGATCACACCACAACCTATTGGGG
TGGTGACAATTTACCCAGGGATCTCTGCTGATGTTATCCGCAATTTTCTCCGCCAGCCGGTGAAAGCGCTGATTCTTCGT
TCTTATGGTGTCGGGAATGCGCCACAAAACAAAGAGTTTTTGCAAGAACTGCAAGAAGCAAGTTCGCGTGGAATTGTGGT
GGTGAATCTTACCCAATGCATGTCTGGCAAAGTGAATATGGGTGGTTATGCAACGGGTAATGCACTGGCCCATGCGGGCG
TCATTGGTGGAGCTGATATGACAGTAGAAGCTACGCTGACGAAGTTGCATTATTTGCTCAGCCAGGGGCTGGATACCCAG
ACTATTCGTCAAGTCATGACTGAAAACTTGCGTGGCGAGCTTACGCCAGATGACTAA

Upstream 100 bases:

>100_bases
AATTTGCTCTCGTTGAGTATTCAACCAAAGGCGGCAAATGCTGCCTTTTTATAATCCTGACGTTATACTTCTCCCCGCTA
ATCACCACTTTAAAACCATC

Downstream 100 bases:

>100_bases
GGAGATTTTGATGACGCATCGCGCTCTGCTACTGGTAGATTTGCAAAATGATTTTTGTGCCGGAGGTGCGCTTGCTGTAC
CGGAAGGTGACAACACCGTT

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MHKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLMDSSDMTPQDWQHIAEDIQAHYD
EYDGFVILHGTDTMAYTASALSFMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLSTPAAPQGQGELIVHPITPQPIGVVTIYPGISADVIRNFLRQPVKALILR
SYGVGNAPQNKEFLQELQEASSRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQGLDTQ
TIRQVMTENLRGELTPDD

Sequences:

>Translated_338_residues
MHKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLMDSSDMTPQDWQHIAEDIQAHYD
EYDGFVILHGTDTMAYTASALSFMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLSTPAAPQGQGELIVHPITPQPIGVVTIYPGISADVIRNFLRQPVKALILR
SYGVGNAPQNKEFLQELQEASSRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQGLDTQ
TIRQVMTENLRGELTPDD
>Mature_338_residues
MHKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLMDSSDMTPQDWQHIAEDIQAHYD
EYDGFVILHGTDTMAYTASALSFMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLSTPAAPQGQGELIVHPITPQPIGVVTIYPGISADVIRNFLRQPVKALILR
SYGVGNAPQNKEFLQELQEASSRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQGLDTQ
TIRQVMTENLRGELTPDD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=359, Percent_Identity=45.9610027855153, Blast_Score=286, Evalue=2e-77,
Organism=Escherichia coli, GI1788065, Length=338, Percent_Identity=94.3786982248521, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI1789327, Length=282, Percent_Identity=28.3687943262411, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=357, Percent_Identity=43.9775910364146, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI17558278, Length=357, Percent_Identity=43.9775910364146, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI17558280, Length=357, Percent_Identity=43.9775910364146, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI193207374, Length=357, Percent_Identity=43.9775910364146, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24639609, Length=369, Percent_Identity=43.360433604336, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24645530, Length=360, Percent_Identity=43.6111111111111, Blast_Score=280, Evalue=8e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37101; Mature: 37101

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLMDS
CCCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
SDMTPQDWQHIAEDIQAHYDEYDGFVILHGTDTMAYTASALSFMLENLSKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPL
HHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRLSTPAAPQGQGELIVHPITPQPIGVVTIYPGISADVIRNFLRQPVKALILR
HHCCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNKEFLQELQEASSRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADM
HCCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCEECCCHHHHCCEECCCCE
TVEATLTKLHYLLSQGLDTQTIRQVMTENLRGELTPDD
EHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLMDS
CCCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
SDMTPQDWQHIAEDIQAHYDEYDGFVILHGTDTMAYTASALSFMLENLSKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPL
HHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRLSTPAAPQGQGELIVHPITPQPIGVVTIYPGISADVIRNFLRQPVKALILR
HHCCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNKEFLQELQEASSRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADM
HCCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCEECCCHHHHCCEECCCCE
TVEATLTKLHYLLSQGLDTQTIRQVMTENLRGELTPDD
EHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]