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The map label for this gene is yojL [H]

Identifier: 218548325

GI number: 218548325

Start: 973776

End: 974831

Strand: Direct

Name: yojL [H]

Synonym: EFER_0946

Alternate gene names: 218548325

Gene position: 973776-974831 (Clockwise)

Preceding gene: 218548324

Following gene: 218548326

Centisome position: 21.22

GC content: 50.85

Gene sequence:

>1056_bases
ATGGAAATAAACTTTGCTCGTGTGGCGCTGTTAGCAGCGGCACTCCTCTTTGTTGGCTGTGATCAAGCCCCGAAATCCAC
TAAAGTCTCATCAGCAGACGTTACGGTACTGGAAGGTAAAACCATGGGTACTTTCTGGCGTGTCAGCGTGGTGGGCATTA
ATGAGAAACGCAGTAACGAGTTGCGTAATCGCATACAGACACAACTGGATGCAGATGATCAGTTGCTCTCAACATATAAA
AAAGATTCGGCACTGATGCGTTTTAACGATACTCACAGTCTCTCTCCCTGGCCGGTAAGTGAAGCGATGGCTGATATCGT
CACCACTGCAATACGAATTGGCGAAAAAACTGAAGGGGCAATGGATGTCACCGTGGGGCCATTGGTTAACCTTTGGGGAT
TTGGCCCCGATAAACAGCCCGTGACGATCCCCTCGCAGGAGCAAATCGACGCTGCAAAAGCGAAAACTGGATTGCAGCAT
CTGACGGTGATTAATCAGTACCACCAGCAATATCTGCAAAAAGATCTGCCAGAGCTATATGTGGATCTTTCCACCGTGGG
AGAAGGTTATGCGGCGGATCATCTGGCACGCTTGATGGAGCAGGAAGGCATTTCCCGCTATCTGGTGTCGGTGGGCGGCG
CGCTGAACAGCCGTGGTATGAACGCTGAAGGCCAGCCGTGGCGGGTAGCGATTCAAAAACCGACCGATCAGGAAAATGCG
GTTCAGGCCGTGGTGGATATCAACGGTCATGGCATTAGCACCTCTGGCAGCTACCGTAACTATTACGAACTGGACGGCAA
ACGTCTTTCTCATGTTATCGATCCGCAAACCGGGCGTCCCATCGAACACAATCTGGTATCCGTGACGGTGATTGCTCCGA
CGGCGCTGGAAGCCGATGCCTGGGATACTGGCTTAATGGTGCTCGGACCGGAGAAAGCGAAAGAAATTGCTCGTCGTGAA
GGACTGGCGGTCTATATGATCACCAAAGAAGGCGATAGTTTTAAAACCTGGATGTCGCCGCAGTTTAAAAGCTTCCTGGT
GAGCGAAAAAAATTAA

Upstream 100 bases:

>100_bases
GGCCTGCGGGCCCTTTTTTCATTGCTTTTTCATACTCTTCAACGCACAAATGCTGATTTTTGGTGTACTCTTGCGCCCGT
TCGCATGAGGATAATCACGT

Downstream 100 bases:

>100_bases
AGCGCAAGATTGTTGGTTTTTGCGTGATGGTGACCGGGCAGACTAAAGGCTATCCTTAACCAGGGAGCTGATGATGAAAA
ACGCCACATGCTTAACTGAC

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MEINFARVALLAAALLFVGCDQAPKSTKVSSADVTVLEGKTMGTFWRVSVVGINEKRSNELRNRIQTQLDADDQLLSTYK
KDSALMRFNDTHSLSPWPVSEAMADIVTTAIRIGEKTEGAMDVTVGPLVNLWGFGPDKQPVTIPSQEQIDAAKAKTGLQH
LTVINQYHQQYLQKDLPELYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNAEGQPWRVAIQKPTDQENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEIARRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN

Sequences:

>Translated_351_residues
MEINFARVALLAAALLFVGCDQAPKSTKVSSADVTVLEGKTMGTFWRVSVVGINEKRSNELRNRIQTQLDADDQLLSTYK
KDSALMRFNDTHSLSPWPVSEAMADIVTTAIRIGEKTEGAMDVTVGPLVNLWGFGPDKQPVTIPSQEQIDAAKAKTGLQH
LTVINQYHQQYLQKDLPELYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNAEGQPWRVAIQKPTDQENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEIARRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
>Mature_351_residues
MEINFARVALLAAALLFVGCDQAPKSTKVSSADVTVLEGKTMGTFWRVSVVGINEKRSNELRNRIQTQLDADDQLLSTYK
KDSALMRFNDTHSLSPWPVSEAMADIVTTAIRIGEKTEGAMDVTVGPLVNLWGFGPDKQPVTIPSQEQIDAAKAKTGLQH
LTVINQYHQQYLQKDLPELYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNAEGQPWRVAIQKPTDQENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEIARRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=351, Percent_Identity=88.8888888888889, Blast_Score=658, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38621; Mature: 38621

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINFARVALLAAALLFVGCDQAPKSTKVSSADVTVLEGKTMGTFWRVSVVGINEKRSNE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCHHHHH
LRNRIQTQLDADDQLLSTYKKDSALMRFNDTHSLSPWPVSEAMADIVTTAIRIGEKTEGA
HHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
MDVTVGPLVNLWGFGPDKQPVTIPSQEQIDAAKAKTGLQHLTVINQYHQQYLQKDLPELY
EEEEHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
VDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNAEGQPWRVAIQKPTDQENA
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHCC
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADA
EEEEEEECCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
WDTGLMVLGPEKAKEIARREGLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
CCCCEEEECHHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MEINFARVALLAAALLFVGCDQAPKSTKVSSADVTVLEGKTMGTFWRVSVVGINEKRSNE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCHHHHH
LRNRIQTQLDADDQLLSTYKKDSALMRFNDTHSLSPWPVSEAMADIVTTAIRIGEKTEGA
HHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
MDVTVGPLVNLWGFGPDKQPVTIPSQEQIDAAKAKTGLQHLTVINQYHQQYLQKDLPELY
EEEEHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
VDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNAEGQPWRVAIQKPTDQENA
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHCC
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADA
EEEEEEECCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
WDTGLMVLGPEKAKEIARREGLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
CCCCEEEECHHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]