Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 218548186

GI number: 218548186

Start: 820527

End: 821855

Strand: Direct

Name: shiA [H]

Synonym: EFER_0803

Alternate gene names: 218548186

Gene position: 820527-821855 (Clockwise)

Preceding gene: 218548185

Following gene: 218548187

Centisome position: 17.88

GC content: 56.36

Gene sequence:

>1329_bases
ATGAGCAATACCGAAGCAGCACCGCACGACCCAAAGCAAATGCGTAAACTGGTGATCGCCTCCGTTCTGGGCAATGCGCT
GGAATGGTATGACTTCTTTCTCTATGGCACGGCGGCGGCGTTAGTCTTCGGCCCGCTCTTTTTCCCGGTCGGCGGCGACC
CGCTACAGGGCACATTACTGGCATTTTCCGGCTTTGCGGTCGGTTTTCTGGCGCGCCCGCTGGGCGGCATTGTCTTTGGT
CACTTAGGCGATCGCTACAGCCGTAAGATGACGTTAATCATGACGTTAACGCTGATGGGCGCGACAACCTTCGTCATCGG
CCTGTTGCCGGTTTACAGCCAGATTGGCATCTGGGCGCCGATTTGTCTGATCACACTGCGTTTTTTACAAGGCGTGGCCT
CGGGCGGCGAGTGGGGCGGCGGCGTATTGATGCTGAGTGAAAACGCCCCGGCATCCAGGCGCGGCTTCTTTACCGCCTGG
AGCCAGATGGGCGTTTCCGGCGGCTTTGTGCTCTCGGCGTTTGCTTTTTACCTGGTGCAGAAGTTGCCGGAATCCGACTT
TATGACCTGGGGCTGGCGCGTGCCGTTCCTGCTGAGCCTGCTGATTTTCCTGGTTGGTGTGTATATTCGCAAAAACATCC
GCGAAAGCGAGGCTTTCCAGCAGGCGAAGCCGGAAGACAAACATGAAAAAATACCGTTGCTGGTGCTGCTGCGCGAACAT
CCGAAAGCGCTGATGCAGGCGATTGCCCTGCGTCTGCCGGAGAACGGCGCATCCTATATTTTCTTCACCTTCTCGGTGGT
GTACGCCAAACATATTGGTATATCAACGAGCATTATTATCAGCGCGGTGACGCTGGCGATGCTGATTGAGTTTTTCTCGA
TCCTGTTCTGGGGTGCGCTCTCCGATCGCATCGGCCTGAAACGCGTCTATTACATCGGCGTGATTGGCCTGCTGGTGATG
GCTTTCCCGTTCTTCTGGCTGGTTTCGACCGGTGAATACAGCTGGATTATGCTGGCGATGTTCCTTGGTCTTCCGGTGTG
TCACGGGGCGATGATTGGCACGCAGCCGTGCATTATGAGTGATTTGTTCCCGGTGCGCGTGCGCTACTCCGGCCTGGCGC
TGGGCCATGAAGTGGGATCTATCTTCTCCGGTGGGCTGGGGCCGATGCTCGCTGTCGCACTGTTAATCGAATTTGACTCC
GCCTGGCCAGTATCGCTGCTGCTGATTGTCTATGCGCTACTGGCGTGGATTGCGTTATTAAGCCTGCCTTCGCAGGAGAC
GCCTGTCCTGAGCAAACCGGGAAACCCCATGCCTTACAATGAAATCTAA

Upstream 100 bases:

>100_bases
TCAGCGAGCAGCCGCGCGGGCTGGTCTATTTTAACCGTCGTTACCATACGGTAGGCGGCGATATTGAGGTGAAAGCCTAA
CCCATTGAGGAGCCTGGCTT

Downstream 100 bases:

>100_bases
ATATTGCCAGTAAGATAATAGACGTCATGCTTAGCCATTGAGTGAAACAGACAGTGGAGTAACAGTTAAAATAGTGGGTA
AACTTTTTTATCCGCTATTT

Product: General substrate transporter:Major facilitator superfamily

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSNTEAAPHDPKQMRKLVIASVLGNALEWYDFFLYGTAAALVFGPLFFPVGGDPLQGTLLAFSGFAVGFLARPLGGIVFG
HLGDRYSRKMTLIMTLTLMGATTFVIGLLPVYSQIGIWAPICLITLRFLQGVASGGEWGGGVLMLSENAPASRRGFFTAW
SQMGVSGGFVLSAFAFYLVQKLPESDFMTWGWRVPFLLSLLIFLVGVYIRKNIRESEAFQQAKPEDKHEKIPLLVLLREH
PKALMQAIALRLPENGASYIFFTFSVVYAKHIGISTSIIISAVTLAMLIEFFSILFWGALSDRIGLKRVYYIGVIGLLVM
AFPFFWLVSTGEYSWIMLAMFLGLPVCHGAMIGTQPCIMSDLFPVRVRYSGLALGHEVGSIFSGGLGPMLAVALLIEFDS
AWPVSLLLIVYALLAWIALLSLPSQETPVLSKPGNPMPYNEI

Sequences:

>Translated_442_residues
MSNTEAAPHDPKQMRKLVIASVLGNALEWYDFFLYGTAAALVFGPLFFPVGGDPLQGTLLAFSGFAVGFLARPLGGIVFG
HLGDRYSRKMTLIMTLTLMGATTFVIGLLPVYSQIGIWAPICLITLRFLQGVASGGEWGGGVLMLSENAPASRRGFFTAW
SQMGVSGGFVLSAFAFYLVQKLPESDFMTWGWRVPFLLSLLIFLVGVYIRKNIRESEAFQQAKPEDKHEKIPLLVLLREH
PKALMQAIALRLPENGASYIFFTFSVVYAKHIGISTSIIISAVTLAMLIEFFSILFWGALSDRIGLKRVYYIGVIGLLVM
AFPFFWLVSTGEYSWIMLAMFLGLPVCHGAMIGTQPCIMSDLFPVRVRYSGLALGHEVGSIFSGGLGPMLAVALLIEFDS
AWPVSLLLIVYALLAWIALLSLPSQETPVLSKPGNPMPYNEI
>Mature_441_residues
SNTEAAPHDPKQMRKLVIASVLGNALEWYDFFLYGTAAALVFGPLFFPVGGDPLQGTLLAFSGFAVGFLARPLGGIVFGH
LGDRYSRKMTLIMTLTLMGATTFVIGLLPVYSQIGIWAPICLITLRFLQGVASGGEWGGGVLMLSENAPASRRGFFTAWS
QMGVSGGFVLSAFAFYLVQKLPESDFMTWGWRVPFLLSLLIFLVGVYIRKNIRESEAFQQAKPEDKHEKIPLLVLLREHP
KALMQAIALRLPENGASYIFFTFSVVYAKHIGISTSIIISAVTLAMLIEFFSILFWGALSDRIGLKRVYYIGVIGLLVMA
FPFFWLVSTGEYSWIMLAMFLGLPVCHGAMIGTQPCIMSDLFPVRVRYSGLALGHEVGSIFSGGLGPMLAVALLIEFDSA
WPVSLLLIVYALLAWIALLSLPSQETPVLSKPGNPMPYNEI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=421, Percent_Identity=37.5296912114014, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1790550, Length=404, Percent_Identity=36.3861386138614, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1789941, Length=420, Percent_Identity=35.2380952380952, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=29.3427230046948, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082404, Length=412, Percent_Identity=27.1844660194175, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87082231, Length=243, Percent_Identity=27.1604938271605, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48461; Mature: 48330

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNTEAAPHDPKQMRKLVIASVLGNALEWYDFFLYGTAAALVFGPLFFPVGGDPLQGTLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
AFSGFAVGFLARPLGGIVFGHLGDRYSRKMTLIMTLTLMGATTFVIGLLPVYSQIGIWAP
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICLITLRFLQGVASGGEWGGGVLMLSENAPASRRGFFTAWSQMGVSGGFVLSAFAFYLVQ
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
KLPESDFMTWGWRVPFLLSLLIFLVGVYIRKNIRESEAFQQAKPEDKHEKIPLLVLLREH
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHC
PKALMQAIALRLPENGASYIFFTFSVVYAKHIGISTSIIISAVTLAMLIEFFSILFWGAL
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDRIGLKRVYYIGVIGLLVMAFPFFWLVSTGEYSWIMLAMFLGLPVCHGAMIGTQPCIMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCHHHCCCCHHHH
DLFPVRVRYSGLALGHEVGSIFSGGLGPMLAVALLIEFDSAWPVSLLLIVYALLAWIALL
CCCHHEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SLPSQETPVLSKPGNPMPYNEI
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]