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The map label for this gene is rutF [H]

Identifier: 218548185

GI number: 218548185

Start: 820006

End: 820506

Strand: Direct

Name: rutF [H]

Synonym: EFER_0802

Alternate gene names: 218548185

Gene position: 820006-820506 (Clockwise)

Preceding gene: 218548184

Following gene: 218548186

Centisome position: 17.87

GC content: 58.88

Gene sequence:

>501_bases
ATGAATTTGCAGACCGAGTTTCGTAATGCGATGGCGCAACTGGGCAGCGCGGTGTCGGTGATCACCACCGATGGCCCGGC
GGGGAAATTTGGCTTTACCGCCTCTGCCGTGTGCAGCGTCACGGATCAACCACCGACCCTGCTGGTGTGTATGAACCGCA
ACTCTTTTGCCAACGGGCACTTCAAGCAAAACGGCGCGCTATGCGTGAACGTGCTTTCCGGCGCGCATCAGGAGTTATCG
GGCATTTTCGCCAATGCGACCCTGCGTTCCGAAGAGCGTTTCGCCTACGACCGCTGGCAGGTGATGGCGAGCGGCGCGCC
GGTACTCAGTTCCGCCGTCGCCAGCTTCGACTGCGTGATTGACGACTGCCATGAAGTGGGCAGCCACACGGTATTTTATT
GCCAGGTGCAGGCGATTCGCATCAGCGAGCAGCCGCGCGGGCTGGTCTATTTTAACCGTCGTTACCATACGGTAGGCGGC
GATATTGAGGTGAAAGCCTAA

Upstream 100 bases:

>100_bases
TATCTGACAGACGAGGAGAAAGCCGATGGCGACCAGATCCTGCTCTGTTGTTCCCGGGCGAAAAGTGCCCGTCTGATTAT
CGATCTCTGAGGATTTCAGC

Downstream 100 bases:

>100_bases
CCCATTGAGGAGCCTGGCTTATGAGCAATACCGAAGCAGCACCGCACGACCCAAAGCAAATGCGTAAACTGGTGATCGCC
TCCGTTCTGGGCAATGCGCT

Product: flavin:NADH reductase ycdH

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MNLQTEFRNAMAQLGSAVSVITTDGPAGKFGFTASAVCSVTDQPPTLLVCMNRNSFANGHFKQNGALCVNVLSGAHQELS
GIFANATLRSEERFAYDRWQVMASGAPVLSSAVASFDCVIDDCHEVGSHTVFYCQVQAIRISEQPRGLVYFNRRYHTVGG
DIEVKA

Sequences:

>Translated_166_residues
MNLQTEFRNAMAQLGSAVSVITTDGPAGKFGFTASAVCSVTDQPPTLLVCMNRNSFANGHFKQNGALCVNVLSGAHQELS
GIFANATLRSEERFAYDRWQVMASGAPVLSSAVASFDCVIDDCHEVGSHTVFYCQVQAIRISEQPRGLVYFNRRYHTVGG
DIEVKA
>Mature_166_residues
MNLQTEFRNAMAQLGSAVSVITTDGPAGKFGFTASAVCSVTDQPPTLLVCMNRNSFANGHFKQNGALCVNVLSGAHQELS
GIFANATLRSEERFAYDRWQVMASGAPVLSSAVASFDCVIDDCHEVGSHTVFYCQVQAIRISEQPRGLVYFNRRYHTVGG
DIEVKA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=160, Percent_Identity=50.625, Blast_Score=164, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 17969; Mature: 17969

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQTEFRNAMAQLGSAVSVITTDGPAGKFGFTASAVCSVTDQPPTLLVCMNRNSFANGH
CCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCE
FKQNGALCVNVLSGAHQELSGIFANATLRSEERFAYDRWQVMASGAPVLSSAVASFDCVI
ECCCCCEEEEEHHCCHHHHHHEEECCEECCCHHHHHHHEEEEECCCCHHHHHHHHHHHHH
DDCHEVGSHTVFYCQVQAIRISEQPRGLVYFNRRYHTVGGDIEVKA
HHHHHCCCCEEEEEEEEEEEECCCCCEEEEECCEEEECCCEEEEEC
>Mature Secondary Structure
MNLQTEFRNAMAQLGSAVSVITTDGPAGKFGFTASAVCSVTDQPPTLLVCMNRNSFANGH
CCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCE
FKQNGALCVNVLSGAHQELSGIFANATLRSEERFAYDRWQVMASGAPVLSSAVASFDCVI
ECCCCCEEEEEHHCCHHHHHHEEECCEECCCHHHHHHHEEEEECCCCHHHHHHHHHHHHH
DDCHEVGSHTVFYCQVQAIRISEQPRGLVYFNRRYHTVGGDIEVKA
HHHHHCCCCEEEEEEEEEEEECCCCCEEEEECCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA