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The map label for this gene is xapR [H]

Identifier: 218548154

GI number: 218548154

Start: 784809

End: 785693

Strand: Direct

Name: xapR [H]

Synonym: EFER_0769

Alternate gene names: 218548154

Gene position: 784809-785693 (Clockwise)

Preceding gene: 218548153

Following gene: 218548155

Centisome position: 17.1

GC content: 48.59

Gene sequence:

>885_bases
ATGGAGCGAAACCCTCGTATAGACCTTAAGTTATTGCGCTACTTTCTTGCGGTGGCAGAAGAGCTGCATTTCGGACGCGC
TGCCGTACGGTTGAATATGTCACAGCCGCCTTTAAGTATTCATATTAAAGAACTGGAACAACAGCTCGGCACACTGCTTT
TTGTTCGTCATTCACGAAGCGTGGCTTTAACACATGCTGGAAAGATCTTGATGGAAGAGTCTCGCCGGCTACTGGCAAGC
GCAAACCATGCGCTTTCTCGTGTGGAGCAAATCGGCCGTGGTGAGGCAGGGCGGATTGAACTCGGCGTAGTTGGCACGGC
AATGTGGGGAAAGATGCGCCCGGCAATGCGCAATTTTTTGAAAGAACATCCAAATGTTGAAGTCCTGTTTCGTGAAAAAT
CACCCGGTATGCAAATGGCGCTACTGGAAAGAAGAGAACTGGATGCCGGGATCTGGCGAATGGCACTACAGCCATCAAGT
GGATTTACCAGTTTACGCTTGCATGAATCCTCTTTTCTTGTGGCGTTACCTGAAGACCATCCAATGGCGAGGTGTGAATC
GATCCCCTTACTGTCTCTCTGTAATGAATACTTCGTTACGCTACCTTCAGCTCACTCTGACTGGGCTTTTTTGCAAAGCG
TTTGCCAACAGGCTGGATTTACACCACTGATCGTACGTGAAGTTGTCGAACCACAGACGGTATTGGCAATGATTAGTATG
GGAATTGGAATTACCTTGATGGCAGAAAGCTATGCACAAATGAACTGGCCTGGGGTGATATTTCGTCCCCTTGATGAGCC
TGTTCCGGCAGATTTATATATTGTTTACGATCAGCAGCAGGCGACACCTGCATTGCAAACGCTGGTTAGGGCATTAACGG
GGTAG

Upstream 100 bases:

>100_bases
GTATTTATGTATATTGGCTATCTGGCGGTAAAATTTATCTTTCACTAATTGATGTGATAAATTTAGAACGTTAATCATCT
TCTCATGGAGCGCAATAAAC

Downstream 100 bases:

>100_bases
ATATTGGTTGAGAGGAATAGCAAAAAAGCGCCTTTAGGGCGCTTTTTTACATTGGTGGGTCGTGCAGGATTCGAACCTGC
GACCAATTGATTAAAAGTCA

Product: DNA-binding transcriptional activator XapR

Products: NA

Alternate protein names: Xanthosine operon regulatory protein [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MERNPRIDLKLLRYFLAVAEELHFGRAAVRLNMSQPPLSIHIKELEQQLGTLLFVRHSRSVALTHAGKILMEESRRLLAS
ANHALSRVEQIGRGEAGRIELGVVGTAMWGKMRPAMRNFLKEHPNVEVLFREKSPGMQMALLERRELDAGIWRMALQPSS
GFTSLRLHESSFLVALPEDHPMARCESIPLLSLCNEYFVTLPSAHSDWAFLQSVCQQAGFTPLIVREVVEPQTVLAMISM
GIGITLMAESYAQMNWPGVIFRPLDEPVPADLYIVYDQQQATPALQTLVRALTG

Sequences:

>Translated_294_residues
MERNPRIDLKLLRYFLAVAEELHFGRAAVRLNMSQPPLSIHIKELEQQLGTLLFVRHSRSVALTHAGKILMEESRRLLAS
ANHALSRVEQIGRGEAGRIELGVVGTAMWGKMRPAMRNFLKEHPNVEVLFREKSPGMQMALLERRELDAGIWRMALQPSS
GFTSLRLHESSFLVALPEDHPMARCESIPLLSLCNEYFVTLPSAHSDWAFLQSVCQQAGFTPLIVREVVEPQTVLAMISM
GIGITLMAESYAQMNWPGVIFRPLDEPVPADLYIVYDQQQATPALQTLVRALTG
>Mature_294_residues
MERNPRIDLKLLRYFLAVAEELHFGRAAVRLNMSQPPLSIHIKELEQQLGTLLFVRHSRSVALTHAGKILMEESRRLLAS
ANHALSRVEQIGRGEAGRIELGVVGTAMWGKMRPAMRNFLKEHPNVEVLFREKSPGMQMALLERRELDAGIWRMALQPSS
GFTSLRLHESSFLVALPEDHPMARCESIPLLSLCNEYFVTLPSAHSDWAFLQSVCQQAGFTPLIVREVVEPQTVLAMISM
GIGITLMAESYAQMNWPGVIFRPLDEPVPADLYIVYDQQQATPALQTLVRALTG

Specific function: Positive regulator required for the expression of xapA and xapB. Binds to the inducer xanthosine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI2367136, Length=293, Percent_Identity=76.4505119453925, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1787879, Length=284, Percent_Identity=34.5070422535211, Blast_Score=160, Evalue=6e-41,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=30.0699300699301, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790399, Length=268, Percent_Identity=28.7313432835821, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=31.0043668122271, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693105, Length=262, Percent_Identity=27.0992366412214, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788748, Length=239, Percent_Identity=25.1046025104602, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=284, Percent_Identity=26.056338028169, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787601, Length=298, Percent_Identity=25.8389261744966, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=25.5172413793103, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788297, Length=255, Percent_Identity=26.2745098039216, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787589, Length=137, Percent_Identity=35.036496350365, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081904, Length=227, Percent_Identity=31.7180616740088, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=242, Percent_Identity=22.3140495867769, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=24.5901639344262, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994962, Length=87, Percent_Identity=39.0804597701149, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33020; Mature: 33020

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNPRIDLKLLRYFLAVAEELHFGRAAVRLNMSQPPLSIHIKELEQQLGTLLFVRHSRS
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHEEEEECCCC
VALTHAGKILMEESRRLLASANHALSRVEQIGRGEAGRIELGVVGTAMWGKMRPAMRNFL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCHHHHHHH
KEHPNVEVLFREKSPGMQMALLERRELDAGIWRMALQPSSGFTSLRLHESSFLVALPEDH
HHCCCEEEEEECCCCCHHHHHHHHHHHCHHHHHEEECCCCCCCEEEEECCEEEEECCCCC
PMARCESIPLLSLCNEYFVTLPSAHSDWAFLQSVCQQAGFTPLIVREVVEPQTVLAMISM
CHHHHCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHH
GIGITLMAESYAQMNWPGVIFRPLDEPVPADLYIVYDQQQATPALQTLVRALTG
CCCEEEEEHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MERNPRIDLKLLRYFLAVAEELHFGRAAVRLNMSQPPLSIHIKELEQQLGTLLFVRHSRS
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHEEEEECCCC
VALTHAGKILMEESRRLLASANHALSRVEQIGRGEAGRIELGVVGTAMWGKMRPAMRNFL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCHHHHHHH
KEHPNVEVLFREKSPGMQMALLERRELDAGIWRMALQPSSGFTSLRLHESSFLVALPEDH
HHCCCEEEEEECCCCCHHHHHHHHHHHCHHHHHEEECCCCCCCEEEEECCEEEEECCCCC
PMARCESIPLLSLCNEYFVTLPSAHSDWAFLQSVCQQAGFTPLIVREVVEPQTVLAMISM
CHHHHCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHH
GIGITLMAESYAQMNWPGVIFRPLDEPVPADLYIVYDQQQATPALQTLVRALTG
CCCEEEEEHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2201776; 9205837; 9278503; 7559336 [H]