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The map label for this gene is ypfI [H]

Identifier: 218548092

GI number: 218548092

Start: 718393

End: 720408

Strand: Direct

Name: ypfI [H]

Synonym: EFER_0703

Alternate gene names: 218548092

Gene position: 718393-720408 (Clockwise)

Preceding gene: 218548091

Following gene: 218548093

Centisome position: 15.66

GC content: 55.31

Gene sequence:

>2016_bases
ATGGCTGAACTGACTGCGCTTCACACATTAACAGCGCAAATGAAACGTGAAGGGATCCGCCGCTTGCTGGTGTTGAGCGG
GGAAGAGGGTTGGTGTTTTGAGCATGCGCTTAAGTTGCGTGATGCCTTACCTGGTGACTGGCTGTGGATTTCGCCGCAGC
CAGTCGCTGAAAACTACTGTTCTCCCTCGACGCTACAAACTTTACTTGGGCGCGAGTTCCGGCATGCGGTATTCGACGCC
CGCCATGGCTTTGATGCCGCTGCCTTTGCAGCACTTAGCGGAACGTTGAAAGCGGGAAGCTGGCTGGTGTTGTTACTCCC
TGCATGGGAAGAGTGGGAGAACCAACCTGATGCCGACTCGCTGCGCTGGAGTGATTGCCCTGACCCTATTGCGACGCCGC
ATTTTGTCCGGCACCTCAAACGCGTACTTACGGCGGATCACGACGCTATCCTCTGGCGGCAAAACCAGCCGTTCACGTTG
ACGCATTTTATTCCCCGTACTGACTGGCACCCCGCGACCGGCGCACCACAACCAGAACAACAGCAACTCTTACAGCAACT
ACTGACCATGCCGCCGGGCGTGGCAGCGGTAACTGCTGCGCGTGGGCGCGGTAAGTCGGCGCTGGCAGGGCAACTCATTT
CTCGTATTGCGGGCAGTGCGATTGTCACCGCGCCTGCAAAAGCGGCAACGGATGTACTGGCACAATTTGCGGGCGAGAAG
TTTCGCTTTATTGCGCCGGATGCTTTGTTAGCCAGCGATGAACAAGCCGACTGGCTGGTGGTTGACGAAGCCGCGGCCAT
ACCTGCGCCGTTGTTGCATCAACTGGTATCGCGTTTTCCTCGAACGTTGTTAACCACTACGGTGCAGGGCTACGAAGGCA
CTGGACGTGGTTTTTTGCTGAAATTTTGCGCTCGCTTTCCGCATTTACACCGTTTCGAATTGCAACAGCCGATCCGCTGG
GCGCAGGGATGCCCGCTGGAAAAAATGGTCAGCGAGGCACTGGTTTTTGACGATGAAAACTTCACCCATACACCACAAGG
CAATATCGTCATTTCCGCATTTGAACAGACGTTATGGAGAAGTGAGTCAGAAACACCGTTAAAGGTTTATCAGTTATTGT
CTGGTGCGCACTACCGGACTTCGCCGCTGGATTTACGCCGTATGATGGATGCACCTGGGCAACATTTTTTCCAGGCGGCT
TGTGAAAACGAGAGCGCCGGGGCGCTGTGGCTGGTGGATGAGGGCGGATTATCTCAAGAACTCAGCCAGGCGGTATGGGC
AGGTTTTCGTCGCCCGCGGGGTAATCTGGTAGCCCAGTCGCTGGCGGCGCACGGCAGCAATCCACTGGCAGCGACATTGC
GCGGACGGCGGGTCAGCAGGATAGCAGTTCATCCAGCTCGTCAGCGTGAAGGCGTTGGGCAACAGCTTATTGCCCGTGCT
TTAAACTATACGCATGACCTCGACTATCTTTCGGTGAGTTTTGGCTACACCGCGGAGTTATGGCGTTTCTGGCAACGCTG
CGGTTTTGTGCTGGTGCGAATGGGTAATCATCGTGAAGCCAGCAGCGGTTGCTATACGGCGATGGCACTGTTACCGATGA
GTGATGCGGGTAAACAGCTGGCTGAACGTGAGCATTACCGTTTACGTCGCGATGCGCATGCTCTCACGCAGTGGAATGGC
GAAACGCTCCCCGTCGATCCACGAAATGATGCCGTCCTTTCTGAGGACGACTGGCTTGAACTTGCCGGTTTTGCTTTCGC
TCATCGTCCGCTATTAACGTCGTTAGGTTGCTTAACGCGTCTGCTACAAACCAGTGAACTGGCATTACCGGCGCTGCGTG
GTCGTTTACAGAAAAACGCCAGTGACGCGCAGTTATGTACCACACTTAAACTTTCAGGCCGCAAGATGTTACTGGCCCGT
CAGCGAGAAGAGGCCGCGCAGGCGCTGTTCGCACTTGATAATGTTTGCACTGAGCGTCTGCGCGATCGCATAACGCAATG
GCAATTTTTTCACTGA

Upstream 100 bases:

>100_bases
CTTCTCAGCAACGCTACAGCTGGTTTAAACGTGGTTTCGACAGCGGCGATCCGGCACAATGCAATACTTTTGGTAAAAGC
ATTTAACTTTCGGGGCAGGG

Downstream 100 bases:

>100_bases
CTCCTTCAATTTTCTCCCATGGTCATTGTGAGTCAGCGGCGTAAAATTAATGACATCAGTTAAGGAGTTCGCCATGAAAC
ATGACCATTTTGTTGTTCAA

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFEHALKLRDALPGDWLWISPQPVAENYCSPSTLQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPAWEEWENQPDADSLRWSDCPDPIATPHFVRHLKRVLTADHDAILWRQNQPFTL
THFIPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSESETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFFQAA
CENESAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIARA
LNYTHDLDYLSVSFGYTAELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAHALTQWNG
ETLPVDPRNDAVLSEDDWLELAGFAFAHRPLLTSLGCLTRLLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLAR
QREEAAQALFALDNVCTERLRDRITQWQFFH

Sequences:

>Translated_671_residues
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFEHALKLRDALPGDWLWISPQPVAENYCSPSTLQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPAWEEWENQPDADSLRWSDCPDPIATPHFVRHLKRVLTADHDAILWRQNQPFTL
THFIPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSESETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFFQAA
CENESAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIARA
LNYTHDLDYLSVSFGYTAELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAHALTQWNG
ETLPVDPRNDAVLSEDDWLELAGFAFAHRPLLTSLGCLTRLLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLAR
QREEAAQALFALDNVCTERLRDRITQWQFFH
>Mature_670_residues
AELTALHTLTAQMKREGIRRLLVLSGEEGWCFEHALKLRDALPGDWLWISPQPVAENYCSPSTLQTLLGREFRHAVFDAR
HGFDAAAFAALSGTLKAGSWLVLLLPAWEEWENQPDADSLRWSDCPDPIATPHFVRHLKRVLTADHDAILWRQNQPFTLT
HFIPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEKF
RFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWA
QGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSESETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFFQAAC
ENESAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIARAL
NYTHDLDYLSVSFGYTAELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAHALTQWNGE
TLPVDPRNDAVLSEDDWLELAGFAFAHRPLLTSLGCLTRLLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLARQ
REEAAQALFALDNVCTERLRDRITQWQFFH

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). It recognizes the wobble base of tRNA(Met), thus distinguishing between tRNA(Met) and the structurally si

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788818, Length=671, Percent_Identity=93.8897168405365, Blast_Score=1284, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17507621, Length=357, Percent_Identity=26.6106442577031, Blast_Score=88, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324197, Length=383, Percent_Identity=25.8485639686684, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640662, Length=357, Percent_Identity=26.6106442577031, Blast_Score=93, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 74986; Mature: 74855

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFEHALKLRDALPGDWLWISPQPVAENYC
CCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHHHCCC
SPSTLQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPAWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVRHLKRVLTADHDAILWRQNQPFTLTHFIPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHH
QQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEEHHHHHHHHC
SESETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFFQAACENESAGALWLVDEGGLSQE
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIARA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCCEEHEECCCCHHHCCHHHHHHHHH
LNYTHDLDYLSVSFGYTAELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HCCCCCCCEEEEECCCHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYRLRRDAHALTQWNGETLPVDPRNDAVLSEDDWLELAGFAFAHRPLLTSLGCLTR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLARQREEAAQALFALDNVCTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC
>Mature Secondary Structure 
AELTALHTLTAQMKREGIRRLLVLSGEEGWCFEHALKLRDALPGDWLWISPQPVAENYC
CCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHHHCCC
SPSTLQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPAWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVRHLKRVLTADHDAILWRQNQPFTLTHFIPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHH
QQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEEHHHHHHHHC
SESETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFFQAACENESAGALWLVDEGGLSQE
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIARA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCCEEHEECCCCHHHCCHHHHHHHHH
LNYTHDLDYLSVSFGYTAELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HCCCCCCCEEEEECCCHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYRLRRDAHALTQWNGETLPVDPRNDAVLSEDDWLELAGFAFAHRPLLTSLGCLTR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLARQREEAAQALFALDNVCTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]