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The map label for this gene is ypfH [H]

Identifier: 218548093

GI number: 218548093

Start: 720482

End: 721180

Strand: Direct

Name: ypfH [H]

Synonym: EFER_0704

Alternate gene names: 218548093

Gene position: 720482-721180 (Clockwise)

Preceding gene: 218548092

Following gene: 218548099

Centisome position: 15.7

GC content: 54.65

Gene sequence:

>699_bases
ATGAAACATGACCATTTTGTTGTTCAAAGCCCGGATAAACCTGCACAACAGTTGTTACTGCTTTTCCACGGTGTCGGGGA
TAACCCGGTAGCGATGGGTGAGATCGGCAGCTGGTTTGCGCCGCTGTTTCCTGATGCACTGGTGGTGAGCATCGGTGGTG
CGGAGCCGAGCGGTAATCCGGCGGGGCGCCAGTGGTTTTCGGTGCAGGGCATAACGGAAGATAATCGCCAGGCGCGTGTG
GATGCCATCATGCCGACGTTTATTGAGACGGTGCGCTACTGGCAGAAACAGAGCGGAGTAGGGGCTAACGCCACGGCGCT
AATCGGTTTCTCACAAGGCGCAATTATGGCGCTGGAGAGCATTAAAGCCGAACCGGGTCTTGCCTCGCGCGTCATTGCCT
TTAACGGACGTTACGCCAGCCTGCCGGAAACCGCGTCTACAGCCACGACAATTCACTTGATCCATGGCGGTGAAGATCCG
GTTATCGATCTGGCGCATGCGGTTGCGGCGCAGGAAGCGTTAATCAGTGTCGGTGGTGATGTGACGCTGGATATCGTGGA
GGATTTGGGCCATGCGATCGACAATCGCAGTATGCAATTTGCCCTGGATCACTTGCGTTATACCATTCCGAAACATTATT
TCGACGAAGCGCTAAGCGGTGGCAAGCCTGGCGATGATGATGTGATTGAAATGATGTAA

Upstream 100 bases:

>100_bases
ATAACGCAATGGCAATTTTTTCACTGACTCCTTCAATTTTCTCCCATGGTCATTGTGAGTCAGCGGCGTAAAATTAATGA
CATCAGTTAAGGAGTTCGCC

Downstream 100 bases:

>100_bases
AAGACAGATTACCAGGCCTGATAAGACGTATGATGCGTCGCATCAGGCATCATGGCCGGATGCGGCGTTAACGCCTGATC
CGGCCACTTAGCTTACTTCT

Product: esterase YpfH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSIGGAEPSGNPAGRQWFSVQGITEDNRQARV
DAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
VIDLAHAVAAQEALISVGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM

Sequences:

>Translated_232_residues
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSIGGAEPSGNPAGRQWFSVQGITEDNRQARV
DAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
VIDLAHAVAAQEALISVGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM
>Mature_232_residues
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSIGGAEPSGNPAGRQWFSVQGITEDNRQARV
DAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
VIDLAHAVAAQEALISVGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM

Specific function: Displays esterase activity toward palmitoyl-CoA and pNP- butyrate [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI87082111, Length=232, Percent_Identity=98.2758620689655, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 24842; Mature: 24842

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSIGGAEPSGNP
CCCCCEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCEEEEEECCCCCCCCC
AGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALES
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
IKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDPVIDLAHAVAAQEALISVGGD
HCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
VTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM
EEHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSIGGAEPSGNP
CCCCCEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCEEEEEECCCCCCCCC
AGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALES
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
IKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDPVIDLAHAVAAQEALISVGGD
HCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
VTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM
EEHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]