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The map label for this gene is hda [H]

Identifier: 218548071

GI number: 218548071

Start: 696388

End: 697113

Strand: Direct

Name: hda [H]

Synonym: EFER_0680

Alternate gene names: 218548071

Gene position: 696388-697113 (Clockwise)

Preceding gene: 218548070

Following gene: 218548074

Centisome position: 15.18

GC content: 50.55

Gene sequence:

>726_bases
GTGAAATCCCTGGTTGAGGTATCTCTGAACACACCGGCACAGCTCTCTTTGCCACTTTATCTTCCTGACGATGAAACCTT
CGCAAGTTTCTGGCCGGGGGATAACTCCTCTTTACTGGCCGCGCTGCAAAACGTGCTGCGTCAGGAACATAGCGGTTACA
TCTATCTCTGGGCGCGCGAAGGTGCGGGGCGCAGCCATTTGCTGCATGCAGCCTGCGCGGAACTTTCTCAACGTGGTGAT
GCCGTTGGCTATGTACCGCTGGATAAGCGTACCTGGTTCGTACCGGAAGTTCTGGACGGAATGGAACAACTTTCGCTGGT
CTGTATCGATAACATTGAATGTATTGCAGGCGACGAATTGTGGGAAATGGCGATTTTTGATCTCTATAACCGCATTCTGG
AGTCAGGCAAAACGCGTCTGTTGATCACCGGTGATCGCCCTCCACGCCAGTTAAACCTGGGGTTGCCGGATCTTGCATCA
CGGCTTGACTGGGGGCAGATTTATAAACTGCAGCCGTTATCTGATGAGGATAAATTGCAAGCATTACAGTTACGCGCCCG
TTTACGCGGCTTTGAGTTACCTGAAGATGTGGGACGCTTCCTGCTGAAACGACTTGATCGTGAAATGCGCACACTGTTTA
TGACACTTGATCAACTTGATCGGGCATCGATTACCGCTCAGCGCAAACTCACCATTCCATTTGTAAAAGAGATCCTGAAA
CTGTAA

Upstream 100 bases:

>100_bases
CAGAAGACGCAGATACACCGCAGAAGTAAAGTAGCAACCGGGCAGTTCCGCTGCCCGGTTCTAACATCATGTAATTCTGT
GGTAAACTTCTCACGATTTT

Downstream 100 bases:

>100_bases
ACGCCGTAGGGTTAGCCACGGCGTTACGTTTTATTAGCTGACAATTTCCAGAACTTGCTCAGGTGGACGACCTATCCGCG
CTTTACCATTCGCCACCACA

Product: DNA replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKSLVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGD
AVGYVPLDKRTWFVPEVLDGMEQLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLAS
RLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILK
L

Sequences:

>Translated_241_residues
MKSLVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGD
AVGYVPLDKRTWFVPEVLDGMEQLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLAS
RLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILK
L
>Mature_241_residues
MKSLVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGD
AVGYVPLDKRTWFVPEVLDGMEQLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLAS
RLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILK
L

Specific function: Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication [H]

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family. HdA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510964, Length=233, Percent_Identity=99.1416309012876, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI2367267, Length=250, Percent_Identity=27.6, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020591
- InterPro:   IPR017788
- InterPro:   IPR013317
- InterPro:   IPR022864 [H]

Pfam domain/function: PF00308 Bac_DnaA [H]

EC number: NA

Molecular weight: Translated: 27480; Mature: 27480

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWARE
CCCEEEEECCCCCEEEEEEECCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
GAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEQLSLVCIDNIECIAGDEL
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHHEEHEECCCEEECCCHH
WEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPLSDEDKLQ
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCCCCHHHHH
ALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECHHHHHHHC
L
C
>Mature Secondary Structure
MKSLVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWARE
CCCEEEEECCCCCEEEEEEECCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
GAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEQLSLVCIDNIECIAGDEL
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHHEEHEECCCEEECCCHH
WEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPLSDEDKLQ
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCCCCHHHHH
ALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA